Spatial Omics Sequencing Based on Microfluidic Array Chips

Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This revi...

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Detalles Bibliográficos
Autores principales: Shi, Jianyu, Pan, Yating, Liu, Xudong, Cao, Wenjian, Mu, Ying, Zhu, Qiangyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377411/
https://www.ncbi.nlm.nih.gov/pubmed/37504111
http://dx.doi.org/10.3390/bios13070712
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author Shi, Jianyu
Pan, Yating
Liu, Xudong
Cao, Wenjian
Mu, Ying
Zhu, Qiangyuan
author_facet Shi, Jianyu
Pan, Yating
Liu, Xudong
Cao, Wenjian
Mu, Ying
Zhu, Qiangyuan
author_sort Shi, Jianyu
collection PubMed
description Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed.
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spelling pubmed-103774112023-07-29 Spatial Omics Sequencing Based on Microfluidic Array Chips Shi, Jianyu Pan, Yating Liu, Xudong Cao, Wenjian Mu, Ying Zhu, Qiangyuan Biosensors (Basel) Review Spatial profiling technologies fill the gap left by the loss of spatial information in traditional single-cell sequencing, showing great application prospects. After just a few years of quick development, spatial profiling technologies have made great progress in resolution and simplicity. This review introduces the development of spatial omics sequencing based on microfluidic array chips and describes barcoding strategies using various microfluidic designs with simplicity and efficiency. At the same time, the pros and cons of each strategy are compared. Moreover, commercialized solutions for spatial profiling are also introduced. In the end, the future perspective of spatial omics sequencing and research directions are discussed. MDPI 2023-07-06 /pmc/articles/PMC10377411/ /pubmed/37504111 http://dx.doi.org/10.3390/bios13070712 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Shi, Jianyu
Pan, Yating
Liu, Xudong
Cao, Wenjian
Mu, Ying
Zhu, Qiangyuan
Spatial Omics Sequencing Based on Microfluidic Array Chips
title Spatial Omics Sequencing Based on Microfluidic Array Chips
title_full Spatial Omics Sequencing Based on Microfluidic Array Chips
title_fullStr Spatial Omics Sequencing Based on Microfluidic Array Chips
title_full_unstemmed Spatial Omics Sequencing Based on Microfluidic Array Chips
title_short Spatial Omics Sequencing Based on Microfluidic Array Chips
title_sort spatial omics sequencing based on microfluidic array chips
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377411/
https://www.ncbi.nlm.nih.gov/pubmed/37504111
http://dx.doi.org/10.3390/bios13070712
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