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Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells
Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanopar...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377447/ https://www.ncbi.nlm.nih.gov/pubmed/37504105 http://dx.doi.org/10.3390/bios13070706 |
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author | Pedrosa, Valber A. Chen, Kangfu George, Thomas J. Fan, Z. Hugh |
author_facet | Pedrosa, Valber A. Chen, Kangfu George, Thomas J. Fan, Z. Hugh |
author_sort | Pedrosa, Valber A. |
collection | PubMed |
description | Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays. |
format | Online Article Text |
id | pubmed-10377447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103774472023-07-29 Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells Pedrosa, Valber A. Chen, Kangfu George, Thomas J. Fan, Z. Hugh Biosensors (Basel) Communication Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays. MDPI 2023-07-04 /pmc/articles/PMC10377447/ /pubmed/37504105 http://dx.doi.org/10.3390/bios13070706 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Pedrosa, Valber A. Chen, Kangfu George, Thomas J. Fan, Z. Hugh Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title | Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title_full | Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title_fullStr | Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title_full_unstemmed | Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title_short | Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells |
title_sort | gold nanoparticle-based microfluidic chips for capture and detection of circulating tumor cells |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377447/ https://www.ncbi.nlm.nih.gov/pubmed/37504105 http://dx.doi.org/10.3390/bios13070706 |
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