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Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes
The potential of cold atmospheric plasma (CAP) in biomedical applications has received significant interest, due to its ability to generate reactive oxygen and nitrogen species (RONS). Upon exposure to living cells, CAP triggers alterations in various cellular components, such as the cell membrane....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377474/ https://www.ncbi.nlm.nih.gov/pubmed/37509079 http://dx.doi.org/10.3390/biom13071043 |
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author | Abduvokhidov, Davronjon Yusupov, Maksudbek Shahzad, Aamir Attri, Pankaj Shiratani, Masaharu Oliveira, Maria C. Razzokov, Jamoliddin |
author_facet | Abduvokhidov, Davronjon Yusupov, Maksudbek Shahzad, Aamir Attri, Pankaj Shiratani, Masaharu Oliveira, Maria C. Razzokov, Jamoliddin |
author_sort | Abduvokhidov, Davronjon |
collection | PubMed |
description | The potential of cold atmospheric plasma (CAP) in biomedical applications has received significant interest, due to its ability to generate reactive oxygen and nitrogen species (RONS). Upon exposure to living cells, CAP triggers alterations in various cellular components, such as the cell membrane. However, the permeation of RONS across nitrated and oxidized membranes remains understudied. To address this gap, we conducted molecular dynamics simulations, to investigate the permeation capabilities of RONS across modified cell membranes. This computational study investigated the translocation processes of less hydrophilic and hydrophilic RONS across the phospholipid bilayer (PLB), with various degrees of oxidation and nitration, and elucidated the impact of RONS on PLB permeability. The simulation results showed that less hydrophilic species, i.e., NO, NO(2), N(2)O(4), and O(3), have a higher penetration ability through nitro-oxidized PLB compared to hydrophilic RONS, i.e., HNO(3), s-cis-HONO, s-trans-HONO, H(2)O(2), HO(2), and OH. In particular, nitro-oxidation of PLB, induced by, e.g., cold atmospheric plasma, has minimal impact on the penetration of free energy barriers of less hydrophilic species, while it lowers these barriers for hydrophilic RONS, thereby enhancing their translocation across nitro-oxidized PLB. This research contributes to a better understanding of the translocation abilities of RONS in the field of plasma biomedical applications and highlights the need for further analysis of their role in intracellular signaling pathways. |
format | Online Article Text |
id | pubmed-10377474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103774742023-07-29 Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes Abduvokhidov, Davronjon Yusupov, Maksudbek Shahzad, Aamir Attri, Pankaj Shiratani, Masaharu Oliveira, Maria C. Razzokov, Jamoliddin Biomolecules Article The potential of cold atmospheric plasma (CAP) in biomedical applications has received significant interest, due to its ability to generate reactive oxygen and nitrogen species (RONS). Upon exposure to living cells, CAP triggers alterations in various cellular components, such as the cell membrane. However, the permeation of RONS across nitrated and oxidized membranes remains understudied. To address this gap, we conducted molecular dynamics simulations, to investigate the permeation capabilities of RONS across modified cell membranes. This computational study investigated the translocation processes of less hydrophilic and hydrophilic RONS across the phospholipid bilayer (PLB), with various degrees of oxidation and nitration, and elucidated the impact of RONS on PLB permeability. The simulation results showed that less hydrophilic species, i.e., NO, NO(2), N(2)O(4), and O(3), have a higher penetration ability through nitro-oxidized PLB compared to hydrophilic RONS, i.e., HNO(3), s-cis-HONO, s-trans-HONO, H(2)O(2), HO(2), and OH. In particular, nitro-oxidation of PLB, induced by, e.g., cold atmospheric plasma, has minimal impact on the penetration of free energy barriers of less hydrophilic species, while it lowers these barriers for hydrophilic RONS, thereby enhancing their translocation across nitro-oxidized PLB. This research contributes to a better understanding of the translocation abilities of RONS in the field of plasma biomedical applications and highlights the need for further analysis of their role in intracellular signaling pathways. MDPI 2023-06-27 /pmc/articles/PMC10377474/ /pubmed/37509079 http://dx.doi.org/10.3390/biom13071043 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abduvokhidov, Davronjon Yusupov, Maksudbek Shahzad, Aamir Attri, Pankaj Shiratani, Masaharu Oliveira, Maria C. Razzokov, Jamoliddin Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title | Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title_full | Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title_fullStr | Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title_full_unstemmed | Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title_short | Unraveling the Transport Properties of RONS across Nitro-Oxidized Membranes |
title_sort | unraveling the transport properties of rons across nitro-oxidized membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377474/ https://www.ncbi.nlm.nih.gov/pubmed/37509079 http://dx.doi.org/10.3390/biom13071043 |
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