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Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips

Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we pres...

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Autores principales: Nam, Sung-Wook, Jeon, Dong-Gyu, Yoon, Young-Ran, Lee, Gang Ho, Chang, Yongmin, Won, Dong Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377501/
https://www.ncbi.nlm.nih.gov/pubmed/37504130
http://dx.doi.org/10.3390/bios13070733
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author Nam, Sung-Wook
Jeon, Dong-Gyu
Yoon, Young-Ran
Lee, Gang Ho
Chang, Yongmin
Won, Dong Il
author_facet Nam, Sung-Wook
Jeon, Dong-Gyu
Yoon, Young-Ran
Lee, Gang Ho
Chang, Yongmin
Won, Dong Il
author_sort Nam, Sung-Wook
collection PubMed
description Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination.
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spelling pubmed-103775012023-07-29 Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips Nam, Sung-Wook Jeon, Dong-Gyu Yoon, Young-Ran Lee, Gang Ho Chang, Yongmin Won, Dong Il Biosensors (Basel) Article Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination. MDPI 2023-07-14 /pmc/articles/PMC10377501/ /pubmed/37504130 http://dx.doi.org/10.3390/bios13070733 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nam, Sung-Wook
Jeon, Dong-Gyu
Yoon, Young-Ran
Lee, Gang Ho
Chang, Yongmin
Won, Dong Il
Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title_full Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title_fullStr Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title_full_unstemmed Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title_short Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
title_sort hemagglutination assay via optical density characterization in 3d microtrap chips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377501/
https://www.ncbi.nlm.nih.gov/pubmed/37504130
http://dx.doi.org/10.3390/bios13070733
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