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Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we pres...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377501/ https://www.ncbi.nlm.nih.gov/pubmed/37504130 http://dx.doi.org/10.3390/bios13070733 |
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author | Nam, Sung-Wook Jeon, Dong-Gyu Yoon, Young-Ran Lee, Gang Ho Chang, Yongmin Won, Dong Il |
author_facet | Nam, Sung-Wook Jeon, Dong-Gyu Yoon, Young-Ran Lee, Gang Ho Chang, Yongmin Won, Dong Il |
author_sort | Nam, Sung-Wook |
collection | PubMed |
description | Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination. |
format | Online Article Text |
id | pubmed-10377501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103775012023-07-29 Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips Nam, Sung-Wook Jeon, Dong-Gyu Yoon, Young-Ran Lee, Gang Ho Chang, Yongmin Won, Dong Il Biosensors (Basel) Article Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination. MDPI 2023-07-14 /pmc/articles/PMC10377501/ /pubmed/37504130 http://dx.doi.org/10.3390/bios13070733 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nam, Sung-Wook Jeon, Dong-Gyu Yoon, Young-Ran Lee, Gang Ho Chang, Yongmin Won, Dong Il Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title | Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title_full | Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title_fullStr | Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title_full_unstemmed | Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title_short | Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips |
title_sort | hemagglutination assay via optical density characterization in 3d microtrap chips |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377501/ https://www.ncbi.nlm.nih.gov/pubmed/37504130 http://dx.doi.org/10.3390/bios13070733 |
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