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Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study

Background: Anti-cytosolic 5′-nucleotidase 1A (anti-cN1A) antibodies were proposed as a biomarker for the diagnosis of inclusion body myositis (IBM), but conflicting specificity and sensitivity evidence limits its use. Our study aimed to assess the diagnostic accuracy of anti-cN1A in a cohort of pat...

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Autores principales: Porcelli, Brunetta, d’Alessandro, Miriana, Gupta, Latika, Grazzini, Silvia, Volpi, Nila, Bacarelli, Maria Romana, Ginanneschi, Federica, Biasi, Giovanni, Bellisai, Francesca, Fabbroni, Marta, Bennett, David, Fabiani, Claudia, Cantarini, Luca, Bargagli, Elena, Frediani, Bruno, Conticini, Edoardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377506/
https://www.ncbi.nlm.nih.gov/pubmed/37509600
http://dx.doi.org/10.3390/biomedicines11071963
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author Porcelli, Brunetta
d’Alessandro, Miriana
Gupta, Latika
Grazzini, Silvia
Volpi, Nila
Bacarelli, Maria Romana
Ginanneschi, Federica
Biasi, Giovanni
Bellisai, Francesca
Fabbroni, Marta
Bennett, David
Fabiani, Claudia
Cantarini, Luca
Bargagli, Elena
Frediani, Bruno
Conticini, Edoardo
author_facet Porcelli, Brunetta
d’Alessandro, Miriana
Gupta, Latika
Grazzini, Silvia
Volpi, Nila
Bacarelli, Maria Romana
Ginanneschi, Federica
Biasi, Giovanni
Bellisai, Francesca
Fabbroni, Marta
Bennett, David
Fabiani, Claudia
Cantarini, Luca
Bargagli, Elena
Frediani, Bruno
Conticini, Edoardo
author_sort Porcelli, Brunetta
collection PubMed
description Background: Anti-cytosolic 5′-nucleotidase 1A (anti-cN1A) antibodies were proposed as a biomarker for the diagnosis of inclusion body myositis (IBM), but conflicting specificity and sensitivity evidence limits its use. Our study aimed to assess the diagnostic accuracy of anti-cN1A in a cohort of patients who underwent a myositis line immunoassay for suspected idiopathic inflammatory myopathies (IIM). We also assessed the agreement between two testing procedures: line immunoassay (LIA) and enzyme-linked immunoassay (ELISA). Materials and methods: We collected retrospective clinical and serological data for 340 patients who underwent a myositis antibody assay using LIA (EUROLINE Autoimmune Inflammatory Myopathies 16 Ag et cN-1A (IgG) line immunoassay) and verification with an anti-cN1A antibody assay using ELISA (IgG) (Euroimmun Lubeck, Germany). Results: The serum samples of 20 (5.88%) patients (15 females, 5 males, mean age 58.76 ± 18.31) tested positive for anti-cN1A using LIA, but only two out of twenty were diagnosed with IBM. Seventeen out of twenty tested positive for anti-cN1A using ELISA (median IQR, 2.9 (1.9–4.18)). Conclusions: Our study suggests excellent concordance between LIA and ELISA for detecting anti-cN1A antibodies. LIA may be a rapid and useful adjunct, and it could even replace ELISA for cN1A assay. However, the high prevalence of diseases other than IBM in our cohort of anti-cN1A-positive patients did not allow us to consider anti-cN1A antibodies as a specific biomarker for IBM.
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spelling pubmed-103775062023-07-29 Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study Porcelli, Brunetta d’Alessandro, Miriana Gupta, Latika Grazzini, Silvia Volpi, Nila Bacarelli, Maria Romana Ginanneschi, Federica Biasi, Giovanni Bellisai, Francesca Fabbroni, Marta Bennett, David Fabiani, Claudia Cantarini, Luca Bargagli, Elena Frediani, Bruno Conticini, Edoardo Biomedicines Article Background: Anti-cytosolic 5′-nucleotidase 1A (anti-cN1A) antibodies were proposed as a biomarker for the diagnosis of inclusion body myositis (IBM), but conflicting specificity and sensitivity evidence limits its use. Our study aimed to assess the diagnostic accuracy of anti-cN1A in a cohort of patients who underwent a myositis line immunoassay for suspected idiopathic inflammatory myopathies (IIM). We also assessed the agreement between two testing procedures: line immunoassay (LIA) and enzyme-linked immunoassay (ELISA). Materials and methods: We collected retrospective clinical and serological data for 340 patients who underwent a myositis antibody assay using LIA (EUROLINE Autoimmune Inflammatory Myopathies 16 Ag et cN-1A (IgG) line immunoassay) and verification with an anti-cN1A antibody assay using ELISA (IgG) (Euroimmun Lubeck, Germany). Results: The serum samples of 20 (5.88%) patients (15 females, 5 males, mean age 58.76 ± 18.31) tested positive for anti-cN1A using LIA, but only two out of twenty were diagnosed with IBM. Seventeen out of twenty tested positive for anti-cN1A using ELISA (median IQR, 2.9 (1.9–4.18)). Conclusions: Our study suggests excellent concordance between LIA and ELISA for detecting anti-cN1A antibodies. LIA may be a rapid and useful adjunct, and it could even replace ELISA for cN1A assay. However, the high prevalence of diseases other than IBM in our cohort of anti-cN1A-positive patients did not allow us to consider anti-cN1A antibodies as a specific biomarker for IBM. MDPI 2023-07-12 /pmc/articles/PMC10377506/ /pubmed/37509600 http://dx.doi.org/10.3390/biomedicines11071963 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Porcelli, Brunetta
d’Alessandro, Miriana
Gupta, Latika
Grazzini, Silvia
Volpi, Nila
Bacarelli, Maria Romana
Ginanneschi, Federica
Biasi, Giovanni
Bellisai, Francesca
Fabbroni, Marta
Bennett, David
Fabiani, Claudia
Cantarini, Luca
Bargagli, Elena
Frediani, Bruno
Conticini, Edoardo
Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title_full Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title_fullStr Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title_full_unstemmed Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title_short Anti-Cytosolic 5′-Nucleotidase 1A in the Diagnosis of Patients with Suspected Idiopathic Inflammatory Myopathies: An Italian Real-Life, Single-Centre Retrospective Study
title_sort anti-cytosolic 5′-nucleotidase 1a in the diagnosis of patients with suspected idiopathic inflammatory myopathies: an italian real-life, single-centre retrospective study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377506/
https://www.ncbi.nlm.nih.gov/pubmed/37509600
http://dx.doi.org/10.3390/biomedicines11071963
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