Cargando…

Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds

Alternative and modified therapeutic approaches are key elements in culminating antibiotic resistance. To this end, an experimental trial was conducted to determine the cytotoxicity and antibacterial potential of composites of magnesium oxide (MgO) nanoparticles and antibiotics stabilized in sodium...

Descripción completa

Detalles Bibliográficos
Autores principales: Murtaza, Maheen, Aqib, Amjad Islam, Khan, Shanza Rauf, Muneer, Afshan, Ali, Muhammad Muddassir, Waseem, Ahmad, Zaheer, Tean, Al-Keridis, Lamya Ahmed, Alshammari, Nawaf, Saeed, Mohd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377686/
https://www.ncbi.nlm.nih.gov/pubmed/37509597
http://dx.doi.org/10.3390/biomedicines11071959
_version_ 1785079579309768704
author Murtaza, Maheen
Aqib, Amjad Islam
Khan, Shanza Rauf
Muneer, Afshan
Ali, Muhammad Muddassir
Waseem, Ahmad
Zaheer, Tean
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Saeed, Mohd
author_facet Murtaza, Maheen
Aqib, Amjad Islam
Khan, Shanza Rauf
Muneer, Afshan
Ali, Muhammad Muddassir
Waseem, Ahmad
Zaheer, Tean
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Saeed, Mohd
author_sort Murtaza, Maheen
collection PubMed
description Alternative and modified therapeutic approaches are key elements in culminating antibiotic resistance. To this end, an experimental trial was conducted to determine the cytotoxicity and antibacterial potential of composites of magnesium oxide (MgO) nanoparticles and antibiotics stabilized in sodium alginate gel against multi-drug-resistant Staphylococcus aureus isolated from a houbara bustard. The characterization of preparations was carried out using X-ray diffraction (XRD), scanning transmissible electron microscopy (STEM), and Fourier-transform infrared spectroscopy (FTIR). The preparations used in this trial consisted of gel-stabilized MgO nanoparticles (MG), gel-stabilized tylosin (GT), gel-stabilized ampicillin (GA), gel-stabilized cefoxitin (GC), gel-stabilized MgO and tylosin (GMT), gel-stabilized MgO and cefoxitin (GMC), and gel-stabilized MgO and ampicillin (GMA). The study presents composites that cause a lesser extent of damage to DNA while significantly enhancing mitotic indices/phases compared to the other single component preparations with respect to the positive control (methyl methanesulphonate). It was also noted that there was a non-significant difference (p > 0.05) between the concentrations of composites and the negative control in the toxicity trial. Studying in parallel trials showed an increased prevalence, potential risk factors, and antibiotic resistance in S. aureus. The composites in a well diffusion trial showed the highest percentage increase in the zone of inhibition in the case of GT (58.42%), followed by GMT (46.15%), GC (40.65%), GMC (40%), GMA (28.72%), and GA (21.75%) compared to the antibiotics alone. A broth microdilution assay showed the lowest minimum inhibitory concentration (MIC) in the case of GMA (9.766 ± 00 µg/mL), followed by that of GT (13.02 ± 5.64 µg/mL), GMC (19.53 ± 0.00 µg/mL), GA (26.04 ± 11.28 µg/mL), GMT (26.04 ± 11.28 µg/mL), MG (39.06 ± 0.00 µg/mL), and GC (39.06 ± 0.00 µg/mL). The study thus concludes the effective tackling of multiple-drug-resistant S. aureus with sodium-alginate-stabilized MgO nanoparticles and antibiotics, whereas toxicity proved to be negligible for these composites.
format Online
Article
Text
id pubmed-10377686
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103776862023-07-29 Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds Murtaza, Maheen Aqib, Amjad Islam Khan, Shanza Rauf Muneer, Afshan Ali, Muhammad Muddassir Waseem, Ahmad Zaheer, Tean Al-Keridis, Lamya Ahmed Alshammari, Nawaf Saeed, Mohd Biomedicines Article Alternative and modified therapeutic approaches are key elements in culminating antibiotic resistance. To this end, an experimental trial was conducted to determine the cytotoxicity and antibacterial potential of composites of magnesium oxide (MgO) nanoparticles and antibiotics stabilized in sodium alginate gel against multi-drug-resistant Staphylococcus aureus isolated from a houbara bustard. The characterization of preparations was carried out using X-ray diffraction (XRD), scanning transmissible electron microscopy (STEM), and Fourier-transform infrared spectroscopy (FTIR). The preparations used in this trial consisted of gel-stabilized MgO nanoparticles (MG), gel-stabilized tylosin (GT), gel-stabilized ampicillin (GA), gel-stabilized cefoxitin (GC), gel-stabilized MgO and tylosin (GMT), gel-stabilized MgO and cefoxitin (GMC), and gel-stabilized MgO and ampicillin (GMA). The study presents composites that cause a lesser extent of damage to DNA while significantly enhancing mitotic indices/phases compared to the other single component preparations with respect to the positive control (methyl methanesulphonate). It was also noted that there was a non-significant difference (p > 0.05) between the concentrations of composites and the negative control in the toxicity trial. Studying in parallel trials showed an increased prevalence, potential risk factors, and antibiotic resistance in S. aureus. The composites in a well diffusion trial showed the highest percentage increase in the zone of inhibition in the case of GT (58.42%), followed by GMT (46.15%), GC (40.65%), GMC (40%), GMA (28.72%), and GA (21.75%) compared to the antibiotics alone. A broth microdilution assay showed the lowest minimum inhibitory concentration (MIC) in the case of GMA (9.766 ± 00 µg/mL), followed by that of GT (13.02 ± 5.64 µg/mL), GMC (19.53 ± 0.00 µg/mL), GA (26.04 ± 11.28 µg/mL), GMT (26.04 ± 11.28 µg/mL), MG (39.06 ± 0.00 µg/mL), and GC (39.06 ± 0.00 µg/mL). The study thus concludes the effective tackling of multiple-drug-resistant S. aureus with sodium-alginate-stabilized MgO nanoparticles and antibiotics, whereas toxicity proved to be negligible for these composites. MDPI 2023-07-11 /pmc/articles/PMC10377686/ /pubmed/37509597 http://dx.doi.org/10.3390/biomedicines11071959 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murtaza, Maheen
Aqib, Amjad Islam
Khan, Shanza Rauf
Muneer, Afshan
Ali, Muhammad Muddassir
Waseem, Ahmad
Zaheer, Tean
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Saeed, Mohd
Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title_full Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title_fullStr Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title_full_unstemmed Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title_short Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against Staphylococcus aureus of Houbara Bustard Birds
title_sort sodium alginate-based mgo nanoparticles coupled antibiotics as safe and effective antimicrobial candidates against staphylococcus aureus of houbara bustard birds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377686/
https://www.ncbi.nlm.nih.gov/pubmed/37509597
http://dx.doi.org/10.3390/biomedicines11071959
work_keys_str_mv AT murtazamaheen sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT aqibamjadislam sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT khanshanzarauf sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT muneerafshan sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT alimuhammadmuddassir sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT waseemahmad sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT zaheertean sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT alkeridislamyaahmed sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT alshammarinawaf sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds
AT saeedmohd sodiumalginatebasedmgonanoparticlescoupledantibioticsassafeandeffectiveantimicrobialcandidatesagainststaphylococcusaureusofhoubarabustardbirds