Cargando…

The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors

The precise differentiation of renal cell tumors (RCTs) is sometimes hard to achieve using standard imaging and histopathological methods, especially for those with eosinophilic features. It has been suggested that the vast overabundance of mitochondria, as a well-known hallmark of eosinophilic cyto...

Descripción completa

Detalles Bibliográficos
Autores principales: Nikolic, Gorana, Zivotic, Maja, Cirovic, Sanja, Despotovic, Sanja, Dundjerovic, Dusko, Radojevic Skodric, Sanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377759/
https://www.ncbi.nlm.nih.gov/pubmed/37510063
http://dx.doi.org/10.3390/diagnostics13142319
_version_ 1785079596824133632
author Nikolic, Gorana
Zivotic, Maja
Cirovic, Sanja
Despotovic, Sanja
Dundjerovic, Dusko
Radojevic Skodric, Sanja
author_facet Nikolic, Gorana
Zivotic, Maja
Cirovic, Sanja
Despotovic, Sanja
Dundjerovic, Dusko
Radojevic Skodric, Sanja
author_sort Nikolic, Gorana
collection PubMed
description The precise differentiation of renal cell tumors (RCTs) is sometimes hard to achieve using standard imaging and histopathological methods, especially for those with eosinophilic features. It has been suggested that the vast overabundance of mitochondria, as a well-known hallmark of eosinophilic cytoplasm, and could be a characteristic of distinct tumor types with opposing clinical outcomes. Thus, we intended to explore the associations between mitochondrial distribution patterns in different RCTs, including 43 cell renal cell carcinomas (ccRCCs), 15 papillary renal cell carcinomas (pRCCs), 20 chromophobe renal cell carcinomas (chRCCs), and 18 renal oncocytomas (ROs). Tumor samples were stained with two anti-mitochondrial antibodies (mitochondrial antibody Ab-2, clone MTC02; prohibitin, II-14-10, MA5-12858), applying immunohistochemistry and immunofluorescence to define mitochondrial distribution patterns (coarse scanty, moderate granular, and diffuse granular). Our results revealed significantly different expression patterns among the investigated RCTs (p < 0.001). The majority of ccRCCs exhibited coarse scanty mitochondrial staining, while all chRCCs had moderate granular expression. Nevertheless, all ROs, all pRCCs, and two cases of ccRCC presenting with higher nuclear grade and eosinophilic cytoplasm had diffuse granular mitochondrial expression. Moreover, with increased distribution of mitochondria, the intensity of staining was higher (p < 0.001). Here we present a strategy that utilizes fast and easy mitochondrial detection to differentiate RO from chRCC, as well as other eosinophilic variants of RCC with high accuracy.
format Online
Article
Text
id pubmed-10377759
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103777592023-07-29 The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors Nikolic, Gorana Zivotic, Maja Cirovic, Sanja Despotovic, Sanja Dundjerovic, Dusko Radojevic Skodric, Sanja Diagnostics (Basel) Article The precise differentiation of renal cell tumors (RCTs) is sometimes hard to achieve using standard imaging and histopathological methods, especially for those with eosinophilic features. It has been suggested that the vast overabundance of mitochondria, as a well-known hallmark of eosinophilic cytoplasm, and could be a characteristic of distinct tumor types with opposing clinical outcomes. Thus, we intended to explore the associations between mitochondrial distribution patterns in different RCTs, including 43 cell renal cell carcinomas (ccRCCs), 15 papillary renal cell carcinomas (pRCCs), 20 chromophobe renal cell carcinomas (chRCCs), and 18 renal oncocytomas (ROs). Tumor samples were stained with two anti-mitochondrial antibodies (mitochondrial antibody Ab-2, clone MTC02; prohibitin, II-14-10, MA5-12858), applying immunohistochemistry and immunofluorescence to define mitochondrial distribution patterns (coarse scanty, moderate granular, and diffuse granular). Our results revealed significantly different expression patterns among the investigated RCTs (p < 0.001). The majority of ccRCCs exhibited coarse scanty mitochondrial staining, while all chRCCs had moderate granular expression. Nevertheless, all ROs, all pRCCs, and two cases of ccRCC presenting with higher nuclear grade and eosinophilic cytoplasm had diffuse granular mitochondrial expression. Moreover, with increased distribution of mitochondria, the intensity of staining was higher (p < 0.001). Here we present a strategy that utilizes fast and easy mitochondrial detection to differentiate RO from chRCC, as well as other eosinophilic variants of RCC with high accuracy. MDPI 2023-07-09 /pmc/articles/PMC10377759/ /pubmed/37510063 http://dx.doi.org/10.3390/diagnostics13142319 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nikolic, Gorana
Zivotic, Maja
Cirovic, Sanja
Despotovic, Sanja
Dundjerovic, Dusko
Radojevic Skodric, Sanja
The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title_full The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title_fullStr The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title_full_unstemmed The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title_short The Utility of Mitochondrial Detection Methods Applied as an Additional Tool for the Differentiation of Renal Cell Tumors
title_sort utility of mitochondrial detection methods applied as an additional tool for the differentiation of renal cell tumors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377759/
https://www.ncbi.nlm.nih.gov/pubmed/37510063
http://dx.doi.org/10.3390/diagnostics13142319
work_keys_str_mv AT nikolicgorana theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT zivoticmaja theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT cirovicsanja theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT despotovicsanja theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT dundjerovicdusko theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT radojevicskodricsanja theutilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT nikolicgorana utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT zivoticmaja utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT cirovicsanja utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT despotovicsanja utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT dundjerovicdusko utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors
AT radojevicskodricsanja utilityofmitochondrialdetectionmethodsappliedasanadditionaltoolforthedifferentiationofrenalcelltumors