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Neutrophil Extracellular Traps Drive Dacryolithiasis
Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, an...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377949/ https://www.ncbi.nlm.nih.gov/pubmed/37508521 http://dx.doi.org/10.3390/cells12141857 |
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author | Zlatar, Leticija Timm, Thomas Lochnit, Günter Bilyy, Rostyslav Bäuerle, Tobias Munoz-Becerra, Marco Schett, Georg Knopf, Jasmin Heichel, Jens Ali, Mohammad Javed Schapher, Mirco Paulsen, Friedrich Herrmann, Martin |
author_facet | Zlatar, Leticija Timm, Thomas Lochnit, Günter Bilyy, Rostyslav Bäuerle, Tobias Munoz-Becerra, Marco Schett, Georg Knopf, Jasmin Heichel, Jens Ali, Mohammad Javed Schapher, Mirco Paulsen, Friedrich Herrmann, Martin |
author_sort | Zlatar, Leticija |
collection | PubMed |
description | Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved. |
format | Online Article Text |
id | pubmed-10377949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103779492023-07-29 Neutrophil Extracellular Traps Drive Dacryolithiasis Zlatar, Leticija Timm, Thomas Lochnit, Günter Bilyy, Rostyslav Bäuerle, Tobias Munoz-Becerra, Marco Schett, Georg Knopf, Jasmin Heichel, Jens Ali, Mohammad Javed Schapher, Mirco Paulsen, Friedrich Herrmann, Martin Cells Article Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved. MDPI 2023-07-14 /pmc/articles/PMC10377949/ /pubmed/37508521 http://dx.doi.org/10.3390/cells12141857 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zlatar, Leticija Timm, Thomas Lochnit, Günter Bilyy, Rostyslav Bäuerle, Tobias Munoz-Becerra, Marco Schett, Georg Knopf, Jasmin Heichel, Jens Ali, Mohammad Javed Schapher, Mirco Paulsen, Friedrich Herrmann, Martin Neutrophil Extracellular Traps Drive Dacryolithiasis |
title | Neutrophil Extracellular Traps Drive Dacryolithiasis |
title_full | Neutrophil Extracellular Traps Drive Dacryolithiasis |
title_fullStr | Neutrophil Extracellular Traps Drive Dacryolithiasis |
title_full_unstemmed | Neutrophil Extracellular Traps Drive Dacryolithiasis |
title_short | Neutrophil Extracellular Traps Drive Dacryolithiasis |
title_sort | neutrophil extracellular traps drive dacryolithiasis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377949/ https://www.ncbi.nlm.nih.gov/pubmed/37508521 http://dx.doi.org/10.3390/cells12141857 |
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