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Neutrophil Extracellular Traps Drive Dacryolithiasis

Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, an...

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Autores principales: Zlatar, Leticija, Timm, Thomas, Lochnit, Günter, Bilyy, Rostyslav, Bäuerle, Tobias, Munoz-Becerra, Marco, Schett, Georg, Knopf, Jasmin, Heichel, Jens, Ali, Mohammad Javed, Schapher, Mirco, Paulsen, Friedrich, Herrmann, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377949/
https://www.ncbi.nlm.nih.gov/pubmed/37508521
http://dx.doi.org/10.3390/cells12141857
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author Zlatar, Leticija
Timm, Thomas
Lochnit, Günter
Bilyy, Rostyslav
Bäuerle, Tobias
Munoz-Becerra, Marco
Schett, Georg
Knopf, Jasmin
Heichel, Jens
Ali, Mohammad Javed
Schapher, Mirco
Paulsen, Friedrich
Herrmann, Martin
author_facet Zlatar, Leticija
Timm, Thomas
Lochnit, Günter
Bilyy, Rostyslav
Bäuerle, Tobias
Munoz-Becerra, Marco
Schett, Georg
Knopf, Jasmin
Heichel, Jens
Ali, Mohammad Javed
Schapher, Mirco
Paulsen, Friedrich
Herrmann, Martin
author_sort Zlatar, Leticija
collection PubMed
description Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved.
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spelling pubmed-103779492023-07-29 Neutrophil Extracellular Traps Drive Dacryolithiasis Zlatar, Leticija Timm, Thomas Lochnit, Günter Bilyy, Rostyslav Bäuerle, Tobias Munoz-Becerra, Marco Schett, Georg Knopf, Jasmin Heichel, Jens Ali, Mohammad Javed Schapher, Mirco Paulsen, Friedrich Herrmann, Martin Cells Article Mucopeptide concretions, previously called dacryoliths, are macroscopic stones that commonly obstruct the lacrimal sac. The mechanism behind dacryolithiasis remains unclear; however, the involvement of various immune cells, including neutrophils, has been confirmed. These findings remain limited, and no information on neutrophil extracellular traps (NETs), essentially involved in the pathogenesis of other lithiases, is available yet. Here, we employ microcomputed tomography, magnetic resonance tomography, histochemistry, mass spectrometry, and enzyme activity analyses to investigate the role of neutrophils and NETs in dacryolithiasis. We classify mucopeptide concretions into three types, with respect to the quantity of cellular and acellular material, polysaccharides, and mucosubstances. We propose the role of neutrophils and NETs within the existing model of gradual formation and growth of mucopeptide concretions, with neutrophils contributing to the initial stages of dacryolithiasis, as they localized on the inner (older) parts of the tissue. As NETs localized on the outer (newer) parts of the tissue, we link their role to the late stages of dacryolithiasis, presumably maintaining the proinflammatory environment and preventing efficient clearance. An abundance of IgG on the surface indicates the involvement of the adaptive immune system later as well. These findings bring new perspectives on dacryolithiasis, in which the innate and adaptive immune system are essentially involved. MDPI 2023-07-14 /pmc/articles/PMC10377949/ /pubmed/37508521 http://dx.doi.org/10.3390/cells12141857 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zlatar, Leticija
Timm, Thomas
Lochnit, Günter
Bilyy, Rostyslav
Bäuerle, Tobias
Munoz-Becerra, Marco
Schett, Georg
Knopf, Jasmin
Heichel, Jens
Ali, Mohammad Javed
Schapher, Mirco
Paulsen, Friedrich
Herrmann, Martin
Neutrophil Extracellular Traps Drive Dacryolithiasis
title Neutrophil Extracellular Traps Drive Dacryolithiasis
title_full Neutrophil Extracellular Traps Drive Dacryolithiasis
title_fullStr Neutrophil Extracellular Traps Drive Dacryolithiasis
title_full_unstemmed Neutrophil Extracellular Traps Drive Dacryolithiasis
title_short Neutrophil Extracellular Traps Drive Dacryolithiasis
title_sort neutrophil extracellular traps drive dacryolithiasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377949/
https://www.ncbi.nlm.nih.gov/pubmed/37508521
http://dx.doi.org/10.3390/cells12141857
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