EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC

The detection of driver gene mutations has become essential for lung cancer; however, insufficient sample sizes make gene panel tests difficult to use. We previously reported that the lung cancer compact panel TM (LCCP) could detect EGFR and MET gene mutations with sputum cytology. To date, the dete...

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Autores principales: Morikawa, Kei, Kinoshita, Kohei, Matsuzawa, Shin, Kida, Hirotaka, Handa, Hiroshi, Inoue, Takeo, Nakamura, Seiji, Sato, Yoshiharu, Mineshita, Masamichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Interesting Images
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378045/
https://www.ncbi.nlm.nih.gov/pubmed/37510070
http://dx.doi.org/10.3390/diagnostics13142327
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author Morikawa, Kei
Kinoshita, Kohei
Matsuzawa, Shin
Kida, Hirotaka
Handa, Hiroshi
Inoue, Takeo
Nakamura, Seiji
Sato, Yoshiharu
Mineshita, Masamichi
author_facet Morikawa, Kei
Kinoshita, Kohei
Matsuzawa, Shin
Kida, Hirotaka
Handa, Hiroshi
Inoue, Takeo
Nakamura, Seiji
Sato, Yoshiharu
Mineshita, Masamichi
author_sort Morikawa, Kei
collection PubMed
description The detection of driver gene mutations has become essential for lung cancer; however, insufficient sample sizes make gene panel tests difficult to use. We previously reported that the lung cancer compact panel TM (LCCP) could detect EGFR and MET gene mutations with sputum cytology. To date, the detection of gene mutation using RNA from sputum samples is considered practically difficult. We report a case in which the EML4-ALK fusion gene was successfully detected from a sputum sample using the LCCP that was just released in Japan as a new next-generation sequencing lung cancer panel, CDx.
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spelling pubmed-103780452023-07-29 EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC Morikawa, Kei Kinoshita, Kohei Matsuzawa, Shin Kida, Hirotaka Handa, Hiroshi Inoue, Takeo Nakamura, Seiji Sato, Yoshiharu Mineshita, Masamichi Diagnostics (Basel) Interesting Images The detection of driver gene mutations has become essential for lung cancer; however, insufficient sample sizes make gene panel tests difficult to use. We previously reported that the lung cancer compact panel TM (LCCP) could detect EGFR and MET gene mutations with sputum cytology. To date, the detection of gene mutation using RNA from sputum samples is considered practically difficult. We report a case in which the EML4-ALK fusion gene was successfully detected from a sputum sample using the LCCP that was just released in Japan as a new next-generation sequencing lung cancer panel, CDx. MDPI 2023-07-10 /pmc/articles/PMC10378045/ /pubmed/37510070 http://dx.doi.org/10.3390/diagnostics13142327 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Interesting Images
Morikawa, Kei
Kinoshita, Kohei
Matsuzawa, Shin
Kida, Hirotaka
Handa, Hiroshi
Inoue, Takeo
Nakamura, Seiji
Sato, Yoshiharu
Mineshita, Masamichi
EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title_full EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title_fullStr EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title_full_unstemmed EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title_short EML4-ALK Gene Mutation Detected with New NGS Lung Cancer Panel CDx Using Sputum Cytology in a Case of Advanced NSCLC
title_sort eml4-alk gene mutation detected with new ngs lung cancer panel cdx using sputum cytology in a case of advanced nsclc
topic Interesting Images
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378045/
https://www.ncbi.nlm.nih.gov/pubmed/37510070
http://dx.doi.org/10.3390/diagnostics13142327
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