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PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We sh...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378293/ https://www.ncbi.nlm.nih.gov/pubmed/37504273 http://dx.doi.org/10.3390/cimb45070357 |
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author | Vdovina, Yulia A. Kurshakova, Maria M. Georgieva, Sofia G. Kopytova, Daria V. |
author_facet | Vdovina, Yulia A. Kurshakova, Maria M. Georgieva, Sofia G. Kopytova, Daria V. |
author_sort | Vdovina, Yulia A. |
collection | PubMed |
description | Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We show that the C-terminal region of the WH domain of PCID2 specifically binds the 3′-noncoding region of the ras2 RNA. While the same region of PCID2 interacts with the Xmas-2 subunit of the TREX-2 complex, PCID2 interacts with RNA independently of Xmas-2. An additional RNA-binding region (M region) was identified in the N-terminal part of the PCI domain and found to bind RNA nonspecifically. Point mutations of evolutionarily conserved amino acid residues in this region completely abolish the PCID2–RNA interaction, while a deletion of the C-terminal domain only partly decreases it. Thus, the specific interaction of PCID2 with RNA requires nonspecific PCID2–RNA binding. |
format | Online Article Text |
id | pubmed-10378293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103782932023-07-29 PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions Vdovina, Yulia A. Kurshakova, Maria M. Georgieva, Sofia G. Kopytova, Daria V. Curr Issues Mol Biol Article Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We show that the C-terminal region of the WH domain of PCID2 specifically binds the 3′-noncoding region of the ras2 RNA. While the same region of PCID2 interacts with the Xmas-2 subunit of the TREX-2 complex, PCID2 interacts with RNA independently of Xmas-2. An additional RNA-binding region (M region) was identified in the N-terminal part of the PCI domain and found to bind RNA nonspecifically. Point mutations of evolutionarily conserved amino acid residues in this region completely abolish the PCID2–RNA interaction, while a deletion of the C-terminal domain only partly decreases it. Thus, the specific interaction of PCID2 with RNA requires nonspecific PCID2–RNA binding. MDPI 2023-07-04 /pmc/articles/PMC10378293/ /pubmed/37504273 http://dx.doi.org/10.3390/cimb45070357 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vdovina, Yulia A. Kurshakova, Maria M. Georgieva, Sofia G. Kopytova, Daria V. PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title | PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title_full | PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title_fullStr | PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title_full_unstemmed | PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title_short | PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions |
title_sort | pcid2 subunit of the drosophila trex-2 complex has two rna-binding regions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378293/ https://www.ncbi.nlm.nih.gov/pubmed/37504273 http://dx.doi.org/10.3390/cimb45070357 |
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