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PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions

Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We sh...

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Autores principales: Vdovina, Yulia A., Kurshakova, Maria M., Georgieva, Sofia G., Kopytova, Daria V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378293/
https://www.ncbi.nlm.nih.gov/pubmed/37504273
http://dx.doi.org/10.3390/cimb45070357
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author Vdovina, Yulia A.
Kurshakova, Maria M.
Georgieva, Sofia G.
Kopytova, Daria V.
author_facet Vdovina, Yulia A.
Kurshakova, Maria M.
Georgieva, Sofia G.
Kopytova, Daria V.
author_sort Vdovina, Yulia A.
collection PubMed
description Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We show that the C-terminal region of the WH domain of PCID2 specifically binds the 3′-noncoding region of the ras2 RNA. While the same region of PCID2 interacts with the Xmas-2 subunit of the TREX-2 complex, PCID2 interacts with RNA independently of Xmas-2. An additional RNA-binding region (M region) was identified in the N-terminal part of the PCI domain and found to bind RNA nonspecifically. Point mutations of evolutionarily conserved amino acid residues in this region completely abolish the PCID2–RNA interaction, while a deletion of the C-terminal domain only partly decreases it. Thus, the specific interaction of PCID2 with RNA requires nonspecific PCID2–RNA binding.
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spelling pubmed-103782932023-07-29 PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions Vdovina, Yulia A. Kurshakova, Maria M. Georgieva, Sofia G. Kopytova, Daria V. Curr Issues Mol Biol Article Drosophila PCID2 is a subunit of the TREX-2 mRNA nuclear export complex. Although the complex has long been studied in eukaryotes, it is still unclear how TREX-2 interacts with mRNA in multicellular organisms. Here, the interaction between Drosophila PCID2 and the ras2 RNA was studied by EMSA. We show that the C-terminal region of the WH domain of PCID2 specifically binds the 3′-noncoding region of the ras2 RNA. While the same region of PCID2 interacts with the Xmas-2 subunit of the TREX-2 complex, PCID2 interacts with RNA independently of Xmas-2. An additional RNA-binding region (M region) was identified in the N-terminal part of the PCI domain and found to bind RNA nonspecifically. Point mutations of evolutionarily conserved amino acid residues in this region completely abolish the PCID2–RNA interaction, while a deletion of the C-terminal domain only partly decreases it. Thus, the specific interaction of PCID2 with RNA requires nonspecific PCID2–RNA binding. MDPI 2023-07-04 /pmc/articles/PMC10378293/ /pubmed/37504273 http://dx.doi.org/10.3390/cimb45070357 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vdovina, Yulia A.
Kurshakova, Maria M.
Georgieva, Sofia G.
Kopytova, Daria V.
PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title_full PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title_fullStr PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title_full_unstemmed PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title_short PCID2 Subunit of the Drosophila TREX-2 Complex Has Two RNA-Binding Regions
title_sort pcid2 subunit of the drosophila trex-2 complex has two rna-binding regions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378293/
https://www.ncbi.nlm.nih.gov/pubmed/37504273
http://dx.doi.org/10.3390/cimb45070357
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