Cargando…

Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice

This study investigated the pharmacological and pathological effects of aqueous mulberry leaf extract on type 1 diabetes mellitus mice induced with an intraperitoneal injection of streptozotocin (STZ). Diabetic mice were randomized into six groups: control (normal group), model, metformin-treated mi...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Liru, Fan, Wei, Qin, Jingping, Guo, Shiyin, Xiao, Hang, Tang, Zhonghai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378407/
https://www.ncbi.nlm.nih.gov/pubmed/37504259
http://dx.doi.org/10.3390/cimb45070343
_version_ 1785079758447443968
author Luo, Liru
Fan, Wei
Qin, Jingping
Guo, Shiyin
Xiao, Hang
Tang, Zhonghai
author_facet Luo, Liru
Fan, Wei
Qin, Jingping
Guo, Shiyin
Xiao, Hang
Tang, Zhonghai
author_sort Luo, Liru
collection PubMed
description This study investigated the pharmacological and pathological effects of aqueous mulberry leaf extract on type 1 diabetes mellitus mice induced with an intraperitoneal injection of streptozotocin (STZ). Diabetic mice were randomized into six groups: control (normal group), model, metformin-treated mice, and high-dose, medium-dose, and low-dose mulberry. The mulberry-treated mice were divided into high-, medium-, and low-dose groups based on the various doses of aqueous mulberry leaf extract during gavage. The efficacy of the six-week intervention was evaluated by measuring levels of fasting plasma glucose, alkaline phosphatase, alanine aminotransferase, aspartate transaminase, blood urea nitrogen, gamma-glutamyl transferase, glucose, high-density lipoprotein cholesterol, lactate dehydrogenase, and low-density lipoprotein cholesterol and recording body weight. Results revealed that mulberry leaf extract exhibited an ideal hypoglycemic effect, and the high-dose group was the most affected. Histology analysis, glycogen staining and apoptosis detection were used to study the extract’s effects on the liver, kidney, and pancreatic cells of diabetic mice, enabling the assessment of its effectiveness and complications on a clinical and theoretical basis. It was shown that a certain concentration of aqueous mulberry leaf extract repaired the islet cells of type 1 diabetes mellitus mice, promoting normal insulin secretion. Herein, it was confirmed that mulberry leaf could be used to develop new hypoglycemic drugs or functional health food with broad applicability.
format Online
Article
Text
id pubmed-10378407
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103784072023-07-29 Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice Luo, Liru Fan, Wei Qin, Jingping Guo, Shiyin Xiao, Hang Tang, Zhonghai Curr Issues Mol Biol Article This study investigated the pharmacological and pathological effects of aqueous mulberry leaf extract on type 1 diabetes mellitus mice induced with an intraperitoneal injection of streptozotocin (STZ). Diabetic mice were randomized into six groups: control (normal group), model, metformin-treated mice, and high-dose, medium-dose, and low-dose mulberry. The mulberry-treated mice were divided into high-, medium-, and low-dose groups based on the various doses of aqueous mulberry leaf extract during gavage. The efficacy of the six-week intervention was evaluated by measuring levels of fasting plasma glucose, alkaline phosphatase, alanine aminotransferase, aspartate transaminase, blood urea nitrogen, gamma-glutamyl transferase, glucose, high-density lipoprotein cholesterol, lactate dehydrogenase, and low-density lipoprotein cholesterol and recording body weight. Results revealed that mulberry leaf extract exhibited an ideal hypoglycemic effect, and the high-dose group was the most affected. Histology analysis, glycogen staining and apoptosis detection were used to study the extract’s effects on the liver, kidney, and pancreatic cells of diabetic mice, enabling the assessment of its effectiveness and complications on a clinical and theoretical basis. It was shown that a certain concentration of aqueous mulberry leaf extract repaired the islet cells of type 1 diabetes mellitus mice, promoting normal insulin secretion. Herein, it was confirmed that mulberry leaf could be used to develop new hypoglycemic drugs or functional health food with broad applicability. MDPI 2023-06-29 /pmc/articles/PMC10378407/ /pubmed/37504259 http://dx.doi.org/10.3390/cimb45070343 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Liru
Fan, Wei
Qin, Jingping
Guo, Shiyin
Xiao, Hang
Tang, Zhonghai
Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title_full Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title_fullStr Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title_full_unstemmed Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title_short Pharmacological and Pathological Effects of Mulberry Leaf Extract on the Treatment of Type 1 Diabetes Mellitus Mice
title_sort pharmacological and pathological effects of mulberry leaf extract on the treatment of type 1 diabetes mellitus mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378407/
https://www.ncbi.nlm.nih.gov/pubmed/37504259
http://dx.doi.org/10.3390/cimb45070343
work_keys_str_mv AT luoliru pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice
AT fanwei pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice
AT qinjingping pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice
AT guoshiyin pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice
AT xiaohang pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice
AT tangzhonghai pharmacologicalandpathologicaleffectsofmulberryleafextractonthetreatmentoftype1diabetesmellitusmice