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Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.

Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial....

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Autores principales: Wang, Qian, Xue, Na, Sun, Chao, Tao, Jing, Mi, Chao, Yuan, Yi, Pan, Xiangwei, Gui, Min, Long, Ronghua, Ding, Renzhan, Li, Shikai, Lin, Liangbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378962/
https://www.ncbi.nlm.nih.gov/pubmed/37510301
http://dx.doi.org/10.3390/genes14071396
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author Wang, Qian
Xue, Na
Sun, Chao
Tao, Jing
Mi, Chao
Yuan, Yi
Pan, Xiangwei
Gui, Min
Long, Ronghua
Ding, Renzhan
Li, Shikai
Lin, Liangbin
author_facet Wang, Qian
Xue, Na
Sun, Chao
Tao, Jing
Mi, Chao
Yuan, Yi
Pan, Xiangwei
Gui, Min
Long, Ronghua
Ding, Renzhan
Li, Shikai
Lin, Liangbin
author_sort Wang, Qian
collection PubMed
description Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial. In this study, a natural mutant of the wild type (ZY511, Zhongyou511) with stable inheritance of the multi-stem trait (ms) was obtained, and it showed abnormal shoot apical meristem (SAM) development and an increased main stem number compared to the WT. Histological and scanning electron microscopy analyses revealed multiple SAMs in the ms mutant, whereas only a single SAM was found in the WT. Transcriptome analyses showed significant alterations in the expression of genes involved in cytokinin (CK) biosynthesis and metabolism pathways in the ms mutant. These findings provide insight into the mechanism of multi-main-stem formation in Brassica napus L. and lay a theoretical foundation for breeding multi-main-stem rapeseed vegetable varieties.
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spelling pubmed-103789622023-07-29 Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. Wang, Qian Xue, Na Sun, Chao Tao, Jing Mi, Chao Yuan, Yi Pan, Xiangwei Gui, Min Long, Ronghua Ding, Renzhan Li, Shikai Lin, Liangbin Genes (Basel) Article Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial. In this study, a natural mutant of the wild type (ZY511, Zhongyou511) with stable inheritance of the multi-stem trait (ms) was obtained, and it showed abnormal shoot apical meristem (SAM) development and an increased main stem number compared to the WT. Histological and scanning electron microscopy analyses revealed multiple SAMs in the ms mutant, whereas only a single SAM was found in the WT. Transcriptome analyses showed significant alterations in the expression of genes involved in cytokinin (CK) biosynthesis and metabolism pathways in the ms mutant. These findings provide insight into the mechanism of multi-main-stem formation in Brassica napus L. and lay a theoretical foundation for breeding multi-main-stem rapeseed vegetable varieties. MDPI 2023-07-03 /pmc/articles/PMC10378962/ /pubmed/37510301 http://dx.doi.org/10.3390/genes14071396 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Qian
Xue, Na
Sun, Chao
Tao, Jing
Mi, Chao
Yuan, Yi
Pan, Xiangwei
Gui, Min
Long, Ronghua
Ding, Renzhan
Li, Shikai
Lin, Liangbin
Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title_full Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title_fullStr Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title_full_unstemmed Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title_short Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
title_sort transcriptomic profiling of shoot apical meristem aberrations in the multi-main-stem mutant (ms) of brassica napus l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378962/
https://www.ncbi.nlm.nih.gov/pubmed/37510301
http://dx.doi.org/10.3390/genes14071396
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