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Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.
Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378962/ https://www.ncbi.nlm.nih.gov/pubmed/37510301 http://dx.doi.org/10.3390/genes14071396 |
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author | Wang, Qian Xue, Na Sun, Chao Tao, Jing Mi, Chao Yuan, Yi Pan, Xiangwei Gui, Min Long, Ronghua Ding, Renzhan Li, Shikai Lin, Liangbin |
author_facet | Wang, Qian Xue, Na Sun, Chao Tao, Jing Mi, Chao Yuan, Yi Pan, Xiangwei Gui, Min Long, Ronghua Ding, Renzhan Li, Shikai Lin, Liangbin |
author_sort | Wang, Qian |
collection | PubMed |
description | Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial. In this study, a natural mutant of the wild type (ZY511, Zhongyou511) with stable inheritance of the multi-stem trait (ms) was obtained, and it showed abnormal shoot apical meristem (SAM) development and an increased main stem number compared to the WT. Histological and scanning electron microscopy analyses revealed multiple SAMs in the ms mutant, whereas only a single SAM was found in the WT. Transcriptome analyses showed significant alterations in the expression of genes involved in cytokinin (CK) biosynthesis and metabolism pathways in the ms mutant. These findings provide insight into the mechanism of multi-main-stem formation in Brassica napus L. and lay a theoretical foundation for breeding multi-main-stem rapeseed vegetable varieties. |
format | Online Article Text |
id | pubmed-10378962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103789622023-07-29 Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. Wang, Qian Xue, Na Sun, Chao Tao, Jing Mi, Chao Yuan, Yi Pan, Xiangwei Gui, Min Long, Ronghua Ding, Renzhan Li, Shikai Lin, Liangbin Genes (Basel) Article Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including the consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial. In this study, a natural mutant of the wild type (ZY511, Zhongyou511) with stable inheritance of the multi-stem trait (ms) was obtained, and it showed abnormal shoot apical meristem (SAM) development and an increased main stem number compared to the WT. Histological and scanning electron microscopy analyses revealed multiple SAMs in the ms mutant, whereas only a single SAM was found in the WT. Transcriptome analyses showed significant alterations in the expression of genes involved in cytokinin (CK) biosynthesis and metabolism pathways in the ms mutant. These findings provide insight into the mechanism of multi-main-stem formation in Brassica napus L. and lay a theoretical foundation for breeding multi-main-stem rapeseed vegetable varieties. MDPI 2023-07-03 /pmc/articles/PMC10378962/ /pubmed/37510301 http://dx.doi.org/10.3390/genes14071396 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Qian Xue, Na Sun, Chao Tao, Jing Mi, Chao Yuan, Yi Pan, Xiangwei Gui, Min Long, Ronghua Ding, Renzhan Li, Shikai Lin, Liangbin Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title | Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title_full | Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title_fullStr | Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title_full_unstemmed | Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title_short | Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L. |
title_sort | transcriptomic profiling of shoot apical meristem aberrations in the multi-main-stem mutant (ms) of brassica napus l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10378962/ https://www.ncbi.nlm.nih.gov/pubmed/37510301 http://dx.doi.org/10.3390/genes14071396 |
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