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Global Protein Profiling in Processed Immunohistochemistry Tissue Sections

Tissue sections, which are widely used in research and diagnostic laboratories and have already been examined by immunohistochemistry (IHC), may subsequently provide a resource for proteomic studies, even though only small amount of protein is available. Therefore, we established a workflow for tand...

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Autores principales: Venz, Simone, von Bohlen und Halbach, Viola, Hentschker, Christian, Junker, Heike, Kuss, Andreas Walter, Sura, Thomas, Krüger, Elke, Völker, Uwe, von Bohlen und Halbach, Oliver, Jensen, Lars Riff, Hammer, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379013/
https://www.ncbi.nlm.nih.gov/pubmed/37511068
http://dx.doi.org/10.3390/ijms241411308
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author Venz, Simone
von Bohlen und Halbach, Viola
Hentschker, Christian
Junker, Heike
Kuss, Andreas Walter
Sura, Thomas
Krüger, Elke
Völker, Uwe
von Bohlen und Halbach, Oliver
Jensen, Lars Riff
Hammer, Elke
author_facet Venz, Simone
von Bohlen und Halbach, Viola
Hentschker, Christian
Junker, Heike
Kuss, Andreas Walter
Sura, Thomas
Krüger, Elke
Völker, Uwe
von Bohlen und Halbach, Oliver
Jensen, Lars Riff
Hammer, Elke
author_sort Venz, Simone
collection PubMed
description Tissue sections, which are widely used in research and diagnostic laboratories and have already been examined by immunohistochemistry (IHC), may subsequently provide a resource for proteomic studies, even though only small amount of protein is available. Therefore, we established a workflow for tandem mass spectrometry-based protein profiling of IHC specimens and characterized defined brain area sections. We investigated the CA1 region of the hippocampus dissected from brain slices of adult C57BL/6J mice. The workflow contains detailed information on sample preparation from brain slices, including removal of antibodies and cover matrices, dissection of region(s) of interest, protein extraction and digestion, mass spectrometry measurement, and data analysis. The Gene Ontology (GO) knowledge base was used for further annotation. Literature searches and Gene Ontology annotation of the detected proteins verify the applicability of this method for global protein profiling using formalin-fixed and embedded material and previously used IHC slides.
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spelling pubmed-103790132023-07-29 Global Protein Profiling in Processed Immunohistochemistry Tissue Sections Venz, Simone von Bohlen und Halbach, Viola Hentschker, Christian Junker, Heike Kuss, Andreas Walter Sura, Thomas Krüger, Elke Völker, Uwe von Bohlen und Halbach, Oliver Jensen, Lars Riff Hammer, Elke Int J Mol Sci Article Tissue sections, which are widely used in research and diagnostic laboratories and have already been examined by immunohistochemistry (IHC), may subsequently provide a resource for proteomic studies, even though only small amount of protein is available. Therefore, we established a workflow for tandem mass spectrometry-based protein profiling of IHC specimens and characterized defined brain area sections. We investigated the CA1 region of the hippocampus dissected from brain slices of adult C57BL/6J mice. The workflow contains detailed information on sample preparation from brain slices, including removal of antibodies and cover matrices, dissection of region(s) of interest, protein extraction and digestion, mass spectrometry measurement, and data analysis. The Gene Ontology (GO) knowledge base was used for further annotation. Literature searches and Gene Ontology annotation of the detected proteins verify the applicability of this method for global protein profiling using formalin-fixed and embedded material and previously used IHC slides. MDPI 2023-07-11 /pmc/articles/PMC10379013/ /pubmed/37511068 http://dx.doi.org/10.3390/ijms241411308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Venz, Simone
von Bohlen und Halbach, Viola
Hentschker, Christian
Junker, Heike
Kuss, Andreas Walter
Sura, Thomas
Krüger, Elke
Völker, Uwe
von Bohlen und Halbach, Oliver
Jensen, Lars Riff
Hammer, Elke
Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title_full Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title_fullStr Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title_full_unstemmed Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title_short Global Protein Profiling in Processed Immunohistochemistry Tissue Sections
title_sort global protein profiling in processed immunohistochemistry tissue sections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379013/
https://www.ncbi.nlm.nih.gov/pubmed/37511068
http://dx.doi.org/10.3390/ijms241411308
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