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Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma

Left-sided and right-sided colorectal cancer (L-CRC and R-CRC) have relatively different clinical pictures and pathophysiological backgrounds. The aim of this study was to investigate the presence of DAB adapter protein 2 (DAB2) as a potential molecular mechanism that contributes to this diversity i...

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Autores principales: Šustić, Ivan, Racetin, Anita, Vukojević, Katarina, Benzon, Benjamin, Tonkić, Ante, Šundov, Željko, Puljiz, Mario, Glavina Durdov, Merica, Filipović, Natalija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379130/
https://www.ncbi.nlm.nih.gov/pubmed/37510211
http://dx.doi.org/10.3390/genes14071306
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author Šustić, Ivan
Racetin, Anita
Vukojević, Katarina
Benzon, Benjamin
Tonkić, Ante
Šundov, Željko
Puljiz, Mario
Glavina Durdov, Merica
Filipović, Natalija
author_facet Šustić, Ivan
Racetin, Anita
Vukojević, Katarina
Benzon, Benjamin
Tonkić, Ante
Šundov, Željko
Puljiz, Mario
Glavina Durdov, Merica
Filipović, Natalija
author_sort Šustić, Ivan
collection PubMed
description Left-sided and right-sided colorectal cancer (L-CRC and R-CRC) have relatively different clinical pictures and pathophysiological backgrounds. The aim of this study was to investigate the presence of DAB adapter protein 2 (DAB2) as a potential molecular mechanism that contributes to this diversity in terms of malignancy and responses to therapy. The expression of the suppressor gene DAB2 in colon cancer has already been analyzed, but its significance has not been fully elucidated. Archived samples from 34 patients who underwent colon cancer surgery were included in this study, with 13 patients with low-grade CRC and 21 with high-grade CRC. Twenty of the tumors were R-CRC, while 14 were L-CRC. DAB2 expression was analyzed immunohistochemically in the tumor tissue and the colon resection margin was used as a control. Tumors were divided into L-CRC and R-CRC, with splenic flexure as the cutoff point for each side. The results showed that R-CRC had lower DAB2 protein expression compared to L-CRC (p = 0.01). High-grade tumors had reduced DAB2 expression compared to low-grade tumors (p = 0.02). These results are consistent with the analysis of DAB2 gene expression data that we exported from the TCGA Colon and Rectal Cancer Study (COADREAD). In 736 samples of colon cancer, lower DAB2 gene expression was found in R-CRC compared to L-CRC (p < 0.0001). DAB2 gene expression was significantly higher in the sigmoid colon than in the cecum and ascending colon (p < 0.01). The analysis confirmed a lower expression of the DAB2 in tumors with positive microsatellite instability (p < 0.001). In conclusion, DAB2 has a role in the biological differences between R-CRC and L-CRC and its therapeutic and diagnostic potential needs to be further examined.
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spelling pubmed-103791302023-07-29 Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma Šustić, Ivan Racetin, Anita Vukojević, Katarina Benzon, Benjamin Tonkić, Ante Šundov, Željko Puljiz, Mario Glavina Durdov, Merica Filipović, Natalija Genes (Basel) Article Left-sided and right-sided colorectal cancer (L-CRC and R-CRC) have relatively different clinical pictures and pathophysiological backgrounds. The aim of this study was to investigate the presence of DAB adapter protein 2 (DAB2) as a potential molecular mechanism that contributes to this diversity in terms of malignancy and responses to therapy. The expression of the suppressor gene DAB2 in colon cancer has already been analyzed, but its significance has not been fully elucidated. Archived samples from 34 patients who underwent colon cancer surgery were included in this study, with 13 patients with low-grade CRC and 21 with high-grade CRC. Twenty of the tumors were R-CRC, while 14 were L-CRC. DAB2 expression was analyzed immunohistochemically in the tumor tissue and the colon resection margin was used as a control. Tumors were divided into L-CRC and R-CRC, with splenic flexure as the cutoff point for each side. The results showed that R-CRC had lower DAB2 protein expression compared to L-CRC (p = 0.01). High-grade tumors had reduced DAB2 expression compared to low-grade tumors (p = 0.02). These results are consistent with the analysis of DAB2 gene expression data that we exported from the TCGA Colon and Rectal Cancer Study (COADREAD). In 736 samples of colon cancer, lower DAB2 gene expression was found in R-CRC compared to L-CRC (p < 0.0001). DAB2 gene expression was significantly higher in the sigmoid colon than in the cecum and ascending colon (p < 0.01). The analysis confirmed a lower expression of the DAB2 in tumors with positive microsatellite instability (p < 0.001). In conclusion, DAB2 has a role in the biological differences between R-CRC and L-CRC and its therapeutic and diagnostic potential needs to be further examined. MDPI 2023-06-21 /pmc/articles/PMC10379130/ /pubmed/37510211 http://dx.doi.org/10.3390/genes14071306 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šustić, Ivan
Racetin, Anita
Vukojević, Katarina
Benzon, Benjamin
Tonkić, Ante
Šundov, Željko
Puljiz, Mario
Glavina Durdov, Merica
Filipović, Natalija
Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title_full Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title_fullStr Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title_full_unstemmed Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title_short Expression Pattern of DAB Adaptor Protein 2 in Left- and Right-Side Colorectal Carcinoma
title_sort expression pattern of dab adaptor protein 2 in left- and right-side colorectal carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379130/
https://www.ncbi.nlm.nih.gov/pubmed/37510211
http://dx.doi.org/10.3390/genes14071306
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