Cargando…

Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer

Although most human endogenous retroviruses (HERVs) have been silenced and lost their ability to translocate because of accumulated mutations during evolution, they still play important roles in human biology. Several studies have demonstrated that HERVs play pathological roles in numerous human dis...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Eun Gyung, Lee, Du Hyeong, Kim, Woo Ryung, Lee, Yun Ju, Bae, Woo Hyeon, Kim, Jung-min, Shin, Hae Jin, Ha, Hongseok, Yi, Joo Mi, Cho, Ssang Goo, Choi, Yung Hyun, Leem, Sun Hee, Cha, Hee Jae, Kim, Sang Woo, Kim, Heui Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379226/
https://www.ncbi.nlm.nih.gov/pubmed/37510314
http://dx.doi.org/10.3390/genes14071410
_version_ 1785079962297958400
author Park, Eun Gyung
Lee, Du Hyeong
Kim, Woo Ryung
Lee, Yun Ju
Bae, Woo Hyeon
Kim, Jung-min
Shin, Hae Jin
Ha, Hongseok
Yi, Joo Mi
Cho, Ssang Goo
Choi, Yung Hyun
Leem, Sun Hee
Cha, Hee Jae
Kim, Sang Woo
Kim, Heui Soo
author_facet Park, Eun Gyung
Lee, Du Hyeong
Kim, Woo Ryung
Lee, Yun Ju
Bae, Woo Hyeon
Kim, Jung-min
Shin, Hae Jin
Ha, Hongseok
Yi, Joo Mi
Cho, Ssang Goo
Choi, Yung Hyun
Leem, Sun Hee
Cha, Hee Jae
Kim, Sang Woo
Kim, Heui Soo
author_sort Park, Eun Gyung
collection PubMed
description Although most human endogenous retroviruses (HERVs) have been silenced and lost their ability to translocate because of accumulated mutations during evolution, they still play important roles in human biology. Several studies have demonstrated that HERVs play pathological roles in numerous human diseases, especially cancer. A few studies have revealed that long non-coding RNAs that are transcribed from HERV sequences affect cancer progression. However, there is no study on microRNAs derived from HERVs related to cancer. In this study, we identified 29 microRNAs (miRNAs) derived from HERV sequences in the human genome. In particular, we discovered that miR-4454, which is HERV-H-derived miRNA, was upregulated in non-muscle-invasive bladder cancer (NMIBC) cells. To figure out the effects of upregulated miR-4454 in NMIBC, genes whose expression was downregulated in NMIBC, as well as tumor suppressor genes, were selected as putative target genes of miR-4454. The dual-luciferase assay was used to determine the negative relationship between miR-4454 and its target genes, DNAJB4 and SASH1, and they were confirmed to be promising target genes of miR-4454. Taken together, this study suggests that the upregulation of miR-4454 derived from HERV-H in NMIBC reduces the expression of the tumor suppressor genes, DNAJB4 and SASH1, to promote NMIBC progression.
format Online
Article
Text
id pubmed-10379226
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103792262023-07-29 Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer Park, Eun Gyung Lee, Du Hyeong Kim, Woo Ryung Lee, Yun Ju Bae, Woo Hyeon Kim, Jung-min Shin, Hae Jin Ha, Hongseok Yi, Joo Mi Cho, Ssang Goo Choi, Yung Hyun Leem, Sun Hee Cha, Hee Jae Kim, Sang Woo Kim, Heui Soo Genes (Basel) Article Although most human endogenous retroviruses (HERVs) have been silenced and lost their ability to translocate because of accumulated mutations during evolution, they still play important roles in human biology. Several studies have demonstrated that HERVs play pathological roles in numerous human diseases, especially cancer. A few studies have revealed that long non-coding RNAs that are transcribed from HERV sequences affect cancer progression. However, there is no study on microRNAs derived from HERVs related to cancer. In this study, we identified 29 microRNAs (miRNAs) derived from HERV sequences in the human genome. In particular, we discovered that miR-4454, which is HERV-H-derived miRNA, was upregulated in non-muscle-invasive bladder cancer (NMIBC) cells. To figure out the effects of upregulated miR-4454 in NMIBC, genes whose expression was downregulated in NMIBC, as well as tumor suppressor genes, were selected as putative target genes of miR-4454. The dual-luciferase assay was used to determine the negative relationship between miR-4454 and its target genes, DNAJB4 and SASH1, and they were confirmed to be promising target genes of miR-4454. Taken together, this study suggests that the upregulation of miR-4454 derived from HERV-H in NMIBC reduces the expression of the tumor suppressor genes, DNAJB4 and SASH1, to promote NMIBC progression. MDPI 2023-07-07 /pmc/articles/PMC10379226/ /pubmed/37510314 http://dx.doi.org/10.3390/genes14071410 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Eun Gyung
Lee, Du Hyeong
Kim, Woo Ryung
Lee, Yun Ju
Bae, Woo Hyeon
Kim, Jung-min
Shin, Hae Jin
Ha, Hongseok
Yi, Joo Mi
Cho, Ssang Goo
Choi, Yung Hyun
Leem, Sun Hee
Cha, Hee Jae
Kim, Sang Woo
Kim, Heui Soo
Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title_full Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title_fullStr Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title_full_unstemmed Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title_short Human Endogenous Retrovirus-H-Derived miR-4454 Inhibits the Expression of DNAJB4 and SASH1 in Non-Muscle-Invasive Bladder Cancer
title_sort human endogenous retrovirus-h-derived mir-4454 inhibits the expression of dnajb4 and sash1 in non-muscle-invasive bladder cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379226/
https://www.ncbi.nlm.nih.gov/pubmed/37510314
http://dx.doi.org/10.3390/genes14071410
work_keys_str_mv AT parkeungyung humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT leeduhyeong humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT kimwooryung humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT leeyunju humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT baewoohyeon humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT kimjungmin humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT shinhaejin humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT hahongseok humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT yijoomi humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT chossanggoo humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT choiyunghyun humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT leemsunhee humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT chaheejae humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT kimsangwoo humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer
AT kimheuisoo humanendogenousretrovirushderivedmir4454inhibitstheexpressionofdnajb4andsash1innonmuscleinvasivebladdercancer