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Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura

Integrin members are cell adhesion receptors that bind to extracellular matrix (ECM) proteins to regulate cell–cell adhesion and cell-ECM adhesion. This process is essential for tissue development and organogenesis. The fusion of two testes is a physiological phenomenon that is required for sperm pr...

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Autores principales: Chen, Yaqing, Chen, Yu, Jian, Baozhu, Feng, Qili, Liu, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379305/
https://www.ncbi.nlm.nih.gov/pubmed/37510356
http://dx.doi.org/10.3390/genes14071452
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author Chen, Yaqing
Chen, Yu
Jian, Baozhu
Feng, Qili
Liu, Lin
author_facet Chen, Yaqing
Chen, Yu
Jian, Baozhu
Feng, Qili
Liu, Lin
author_sort Chen, Yaqing
collection PubMed
description Integrin members are cell adhesion receptors that bind to extracellular matrix (ECM) proteins to regulate cell–cell adhesion and cell-ECM adhesion. This process is essential for tissue development and organogenesis. The fusion of two testes is a physiological phenomenon that is required for sperm production and effective reproduction in many Lepidoptera. However, the molecular mechanism of testicular fusion is unclear. In Spodoptera litura, two separated testes fuse into a single testis during the larva-to-pupa transformation. We identified five α and five β integrin subunits that were closely associated with testicular fusion. Integrin α1 and α2 belong to the position-specific 1 (PS1) and PS2 groups, respectively. Integrin α3, αPS1/αPS2, and αPS3 were clustered into the PS3 group. Integrin β1 belonged to the insect β group, and β2, β3, and β5 were clustered in the βν group. Among these integrins, α1, α2, α3, αPS1/PS2, αPS3, β1, and β4 subunits were highly expressed when the testes fused. However, their expression levels were much lower before and after the fusion of the testis. The qRT-PCR and immunohistochemistry analyses indicated that integrin β1 mRNA and the protein were highly expressed in the peritoneal sheath of the testis, particularly when the testes fused. These results indicate that integrins might participate in S. litura testicular fusion.
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spelling pubmed-103793052023-07-29 Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura Chen, Yaqing Chen, Yu Jian, Baozhu Feng, Qili Liu, Lin Genes (Basel) Article Integrin members are cell adhesion receptors that bind to extracellular matrix (ECM) proteins to regulate cell–cell adhesion and cell-ECM adhesion. This process is essential for tissue development and organogenesis. The fusion of two testes is a physiological phenomenon that is required for sperm production and effective reproduction in many Lepidoptera. However, the molecular mechanism of testicular fusion is unclear. In Spodoptera litura, two separated testes fuse into a single testis during the larva-to-pupa transformation. We identified five α and five β integrin subunits that were closely associated with testicular fusion. Integrin α1 and α2 belong to the position-specific 1 (PS1) and PS2 groups, respectively. Integrin α3, αPS1/αPS2, and αPS3 were clustered into the PS3 group. Integrin β1 belonged to the insect β group, and β2, β3, and β5 were clustered in the βν group. Among these integrins, α1, α2, α3, αPS1/PS2, αPS3, β1, and β4 subunits were highly expressed when the testes fused. However, their expression levels were much lower before and after the fusion of the testis. The qRT-PCR and immunohistochemistry analyses indicated that integrin β1 mRNA and the protein were highly expressed in the peritoneal sheath of the testis, particularly when the testes fused. These results indicate that integrins might participate in S. litura testicular fusion. MDPI 2023-07-15 /pmc/articles/PMC10379305/ /pubmed/37510356 http://dx.doi.org/10.3390/genes14071452 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yaqing
Chen, Yu
Jian, Baozhu
Feng, Qili
Liu, Lin
Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title_full Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title_fullStr Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title_full_unstemmed Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title_short Identification and Expression of Integrins during Testicular Fusion in Spodoptera litura
title_sort identification and expression of integrins during testicular fusion in spodoptera litura
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379305/
https://www.ncbi.nlm.nih.gov/pubmed/37510356
http://dx.doi.org/10.3390/genes14071452
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