Cargando…
Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses
Raffinose family oligosaccharides (RFOs) are very important for plant growth, development, and abiotic stress tolerance. Galactinol synthase (GolS) and raffinose synthase (RFS) are critical enzymes involved in RFO biosynthesis. However, the whole-genome identification and stress responses of their c...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379439/ https://www.ncbi.nlm.nih.gov/pubmed/37510251 http://dx.doi.org/10.3390/genes14071344 |
_version_ | 1785080020051427328 |
---|---|
author | Jing, Quankai Chen, Airu Lv, Zhaoyan Dong, Zhihao Wang, Lixia Meng, Xiaoke Feng, Yue Wan, Yu Su, Chengyun Cui, Yanjie Xu, Wenjuan Hou, Hualan Zhu, Xiaobiao |
author_facet | Jing, Quankai Chen, Airu Lv, Zhaoyan Dong, Zhihao Wang, Lixia Meng, Xiaoke Feng, Yue Wan, Yu Su, Chengyun Cui, Yanjie Xu, Wenjuan Hou, Hualan Zhu, Xiaobiao |
author_sort | Jing, Quankai |
collection | PubMed |
description | Raffinose family oligosaccharides (RFOs) are very important for plant growth, development, and abiotic stress tolerance. Galactinol synthase (GolS) and raffinose synthase (RFS) are critical enzymes involved in RFO biosynthesis. However, the whole-genome identification and stress responses of their coding genes in potato remain unexplored. In this study, four StGolS and nine StRFS genes were identified and classified into three and five subgroups, respectively. Remarkably, a total of two StGolS and four StRFS genes in potato were identified to form collinear pairs with those in both Arabidopsis and tomato, respectively. Subsequent analysis revealed that StGolS4 exhibited significantly high expression levels in transport-related tissues, PEG-6000, and ABA treatments, with remarkable upregulation under salt stress. Additionally, StRFS5 showed similar responses to StGolS4, but StRFS4 and StRFS8 gene expression increased significantly under salt treatment and decreased in PEG-6000 and ABA treatments. Overall, these results lay a foundation for further research on the functional characteristics and molecular mechanisms of these two gene families in response to ABA, salt, and drought stresses, and provide a theoretical foundation and new gene resources for the abiotic-stress-tolerant breeding of potato. |
format | Online Article Text |
id | pubmed-10379439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103794392023-07-29 Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses Jing, Quankai Chen, Airu Lv, Zhaoyan Dong, Zhihao Wang, Lixia Meng, Xiaoke Feng, Yue Wan, Yu Su, Chengyun Cui, Yanjie Xu, Wenjuan Hou, Hualan Zhu, Xiaobiao Genes (Basel) Article Raffinose family oligosaccharides (RFOs) are very important for plant growth, development, and abiotic stress tolerance. Galactinol synthase (GolS) and raffinose synthase (RFS) are critical enzymes involved in RFO biosynthesis. However, the whole-genome identification and stress responses of their coding genes in potato remain unexplored. In this study, four StGolS and nine StRFS genes were identified and classified into three and five subgroups, respectively. Remarkably, a total of two StGolS and four StRFS genes in potato were identified to form collinear pairs with those in both Arabidopsis and tomato, respectively. Subsequent analysis revealed that StGolS4 exhibited significantly high expression levels in transport-related tissues, PEG-6000, and ABA treatments, with remarkable upregulation under salt stress. Additionally, StRFS5 showed similar responses to StGolS4, but StRFS4 and StRFS8 gene expression increased significantly under salt treatment and decreased in PEG-6000 and ABA treatments. Overall, these results lay a foundation for further research on the functional characteristics and molecular mechanisms of these two gene families in response to ABA, salt, and drought stresses, and provide a theoretical foundation and new gene resources for the abiotic-stress-tolerant breeding of potato. MDPI 2023-06-26 /pmc/articles/PMC10379439/ /pubmed/37510251 http://dx.doi.org/10.3390/genes14071344 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jing, Quankai Chen, Airu Lv, Zhaoyan Dong, Zhihao Wang, Lixia Meng, Xiaoke Feng, Yue Wan, Yu Su, Chengyun Cui, Yanjie Xu, Wenjuan Hou, Hualan Zhu, Xiaobiao Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title | Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title_full | Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title_fullStr | Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title_full_unstemmed | Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title_short | Systematic Analysis of Galactinol Synthase and Raffinose Synthase Gene Families in Potato and Their Expression Patterns in Development and Abiotic Stress Responses |
title_sort | systematic analysis of galactinol synthase and raffinose synthase gene families in potato and their expression patterns in development and abiotic stress responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379439/ https://www.ncbi.nlm.nih.gov/pubmed/37510251 http://dx.doi.org/10.3390/genes14071344 |
work_keys_str_mv | AT jingquankai systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT chenairu systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT lvzhaoyan systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT dongzhihao systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT wanglixia systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT mengxiaoke systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT fengyue systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT wanyu systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT suchengyun systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT cuiyanjie systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT xuwenjuan systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT houhualan systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses AT zhuxiaobiao systematicanalysisofgalactinolsynthaseandraffinosesynthasegenefamiliesinpotatoandtheirexpressionpatternsindevelopmentandabioticstressresponses |