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The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation

Self-incompatible pitaya varieties have low fruit-setting rates under natural conditions, leading to higher production costs and hindering industrial prosperity. Through transcriptome sequencing, we obtained the 36,900 longest transcripts (including 9167 new transcripts) from 60 samples of flowers....

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Autores principales: Wang, Zhouwen, Wang, Meng, Ding, Yi, Li, Tao, Jiang, Senrong, Kang, Shaoling, Wei, Shuangshuang, Xie, Jun, Huang, Jiaquan, Hu, Wenbin, Li, Hongli, Tang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379629/
https://www.ncbi.nlm.nih.gov/pubmed/37511162
http://dx.doi.org/10.3390/ijms241411406
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author Wang, Zhouwen
Wang, Meng
Ding, Yi
Li, Tao
Jiang, Senrong
Kang, Shaoling
Wei, Shuangshuang
Xie, Jun
Huang, Jiaquan
Hu, Wenbin
Li, Hongli
Tang, Hua
author_facet Wang, Zhouwen
Wang, Meng
Ding, Yi
Li, Tao
Jiang, Senrong
Kang, Shaoling
Wei, Shuangshuang
Xie, Jun
Huang, Jiaquan
Hu, Wenbin
Li, Hongli
Tang, Hua
author_sort Wang, Zhouwen
collection PubMed
description Self-incompatible pitaya varieties have low fruit-setting rates under natural conditions, leading to higher production costs and hindering industrial prosperity. Through transcriptome sequencing, we obtained the 36,900 longest transcripts (including 9167 new transcripts) from 60 samples of flowers. Samples were collected pre- and post-pollination (at 0 h, 0.5 h, 2 h, 4 h, and 12 h) from two varieties of pitaya (self-compatible Jindu No. 1 and self-incompatible Cu Sha). Using the RNA-Seq data and comparison of reference genomes, we annotated 28,817 genes in various databases, and 1740 genes were optimized in their structure for annotation. There were significant differences in the expression of differentially expressed genes (DEGs) in the pitaya stigmas under different pollination types, especially at the late post-pollination stage, where the expression of protease genes increasedal significantly under cross-pollination. We identified DEGs involved in the ribosomal, ubiquitination-mediated, and phyto-signaling pathways that may be involved in pitaya SI regulation. Based on the available transcriptome data and bioinformatics analysis, we tentatively identified HuS-RNase2 as a candidate gynogenetic S gene in the pitaya GSI system.
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spelling pubmed-103796292023-07-29 The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation Wang, Zhouwen Wang, Meng Ding, Yi Li, Tao Jiang, Senrong Kang, Shaoling Wei, Shuangshuang Xie, Jun Huang, Jiaquan Hu, Wenbin Li, Hongli Tang, Hua Int J Mol Sci Article Self-incompatible pitaya varieties have low fruit-setting rates under natural conditions, leading to higher production costs and hindering industrial prosperity. Through transcriptome sequencing, we obtained the 36,900 longest transcripts (including 9167 new transcripts) from 60 samples of flowers. Samples were collected pre- and post-pollination (at 0 h, 0.5 h, 2 h, 4 h, and 12 h) from two varieties of pitaya (self-compatible Jindu No. 1 and self-incompatible Cu Sha). Using the RNA-Seq data and comparison of reference genomes, we annotated 28,817 genes in various databases, and 1740 genes were optimized in their structure for annotation. There were significant differences in the expression of differentially expressed genes (DEGs) in the pitaya stigmas under different pollination types, especially at the late post-pollination stage, where the expression of protease genes increasedal significantly under cross-pollination. We identified DEGs involved in the ribosomal, ubiquitination-mediated, and phyto-signaling pathways that may be involved in pitaya SI regulation. Based on the available transcriptome data and bioinformatics analysis, we tentatively identified HuS-RNase2 as a candidate gynogenetic S gene in the pitaya GSI system. MDPI 2023-07-13 /pmc/articles/PMC10379629/ /pubmed/37511162 http://dx.doi.org/10.3390/ijms241411406 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zhouwen
Wang, Meng
Ding, Yi
Li, Tao
Jiang, Senrong
Kang, Shaoling
Wei, Shuangshuang
Xie, Jun
Huang, Jiaquan
Hu, Wenbin
Li, Hongli
Tang, Hua
The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title_full The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title_fullStr The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title_full_unstemmed The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title_short The Pitaya Flower Tissue’s Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation
title_sort pitaya flower tissue’s gene differential expression analysis between self-incompatible and self-compatible varieties for the identification of genes involved in self-incompatibility regulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379629/
https://www.ncbi.nlm.nih.gov/pubmed/37511162
http://dx.doi.org/10.3390/ijms241411406
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