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Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures
Aristolochia manshuriensis is a relic liana, which is widely used in traditional Chinese herbal medicine and is endemic to the Manchurian floristic region. Since this plant is rare and slow-growing, alternative sources of its valuable compounds could be explored. Herein, we established hairy root cu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379662/ https://www.ncbi.nlm.nih.gov/pubmed/37511000 http://dx.doi.org/10.3390/ijms241411240 |
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author | Shkryl, Yury N. Tchernoded, Galina K. Yugay, Yulia A. Grigorchuk, Valeria P. Sorokina, Maria R. Gorpenchenko, Tatiana Y. Kudinova, Olesya D. Degtyarenko, Anton I. Onishchenko, Maria S. Shved, Nikita A. Kumeiko, Vadim V. Bulgakov, Victor P. |
author_facet | Shkryl, Yury N. Tchernoded, Galina K. Yugay, Yulia A. Grigorchuk, Valeria P. Sorokina, Maria R. Gorpenchenko, Tatiana Y. Kudinova, Olesya D. Degtyarenko, Anton I. Onishchenko, Maria S. Shved, Nikita A. Kumeiko, Vadim V. Bulgakov, Victor P. |
author_sort | Shkryl, Yury N. |
collection | PubMed |
description | Aristolochia manshuriensis is a relic liana, which is widely used in traditional Chinese herbal medicine and is endemic to the Manchurian floristic region. Since this plant is rare and slow-growing, alternative sources of its valuable compounds could be explored. Herein, we established hairy root cultures of A. manshuriensis transformed with Agrobacterium rhizogenes root oncogenic loci (rol)B and rolC genes. The accumulation of nitrogenous secondary metabolites significantly improved in transgenic cell cultures. Specifically, the production of magnoflorine reached up to 5.72 mg/g of dry weight, which is 5.8 times higher than the control calli and 1.7 times higher than in wild-growing liana. Simultaneously, the amounts of aristolochic acids I and II, responsible for the toxicity of Aristolochia species, decreased by more than 10 fold. Consequently, the hairy root extracts demonstrated pronounced cytotoxicity against human glioblastoma cells (U-87 MG), cervical cancer cells (HeLa CCL-2), and colon carcinoma (RKO) cells. However, they did not exhibit significant activity against triple-negative breast cancer cells (MDA-MB-231). Our findings suggest that hairy root cultures of A. manshuriensis could be considered for the rational production of valuable A. manshuriensis compounds by the modification of secondary metabolism. |
format | Online Article Text |
id | pubmed-10379662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103796622023-07-29 Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures Shkryl, Yury N. Tchernoded, Galina K. Yugay, Yulia A. Grigorchuk, Valeria P. Sorokina, Maria R. Gorpenchenko, Tatiana Y. Kudinova, Olesya D. Degtyarenko, Anton I. Onishchenko, Maria S. Shved, Nikita A. Kumeiko, Vadim V. Bulgakov, Victor P. Int J Mol Sci Article Aristolochia manshuriensis is a relic liana, which is widely used in traditional Chinese herbal medicine and is endemic to the Manchurian floristic region. Since this plant is rare and slow-growing, alternative sources of its valuable compounds could be explored. Herein, we established hairy root cultures of A. manshuriensis transformed with Agrobacterium rhizogenes root oncogenic loci (rol)B and rolC genes. The accumulation of nitrogenous secondary metabolites significantly improved in transgenic cell cultures. Specifically, the production of magnoflorine reached up to 5.72 mg/g of dry weight, which is 5.8 times higher than the control calli and 1.7 times higher than in wild-growing liana. Simultaneously, the amounts of aristolochic acids I and II, responsible for the toxicity of Aristolochia species, decreased by more than 10 fold. Consequently, the hairy root extracts demonstrated pronounced cytotoxicity against human glioblastoma cells (U-87 MG), cervical cancer cells (HeLa CCL-2), and colon carcinoma (RKO) cells. However, they did not exhibit significant activity against triple-negative breast cancer cells (MDA-MB-231). Our findings suggest that hairy root cultures of A. manshuriensis could be considered for the rational production of valuable A. manshuriensis compounds by the modification of secondary metabolism. MDPI 2023-07-08 /pmc/articles/PMC10379662/ /pubmed/37511000 http://dx.doi.org/10.3390/ijms241411240 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shkryl, Yury N. Tchernoded, Galina K. Yugay, Yulia A. Grigorchuk, Valeria P. Sorokina, Maria R. Gorpenchenko, Tatiana Y. Kudinova, Olesya D. Degtyarenko, Anton I. Onishchenko, Maria S. Shved, Nikita A. Kumeiko, Vadim V. Bulgakov, Victor P. Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title | Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title_full | Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title_fullStr | Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title_full_unstemmed | Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title_short | Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures |
title_sort | enhanced production of nitrogenated metabolites with anticancer potential in aristolochia manshuriensis hairy root cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379662/ https://www.ncbi.nlm.nih.gov/pubmed/37511000 http://dx.doi.org/10.3390/ijms241411240 |
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