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MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter

Iron(Fe) is a trace metal element necessary for plant growth, but excess iron is harmful to plants. Natural resistance-associated macrophage proteins (NRAMPs) are important for divalent metal transport in plants. In this study, we isolated the MsNRAMP2 (MN_547960) gene from alfalfa, the perennial le...

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Autores principales: Li, Run-Tian, Yang, Yun-Jiao, Liu, Wen-Jun, Liang, Wen-Wei, Zhang, Miao, Dong, Shi-Chen, Shu, Yong-Jun, Guo, Dong-Lin, Guo, Chang-Hong, Bi, Ying-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379929/
https://www.ncbi.nlm.nih.gov/pubmed/37511038
http://dx.doi.org/10.3390/ijms241411278
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author Li, Run-Tian
Yang, Yun-Jiao
Liu, Wen-Jun
Liang, Wen-Wei
Zhang, Miao
Dong, Shi-Chen
Shu, Yong-Jun
Guo, Dong-Lin
Guo, Chang-Hong
Bi, Ying-Dong
author_facet Li, Run-Tian
Yang, Yun-Jiao
Liu, Wen-Jun
Liang, Wen-Wei
Zhang, Miao
Dong, Shi-Chen
Shu, Yong-Jun
Guo, Dong-Lin
Guo, Chang-Hong
Bi, Ying-Dong
author_sort Li, Run-Tian
collection PubMed
description Iron(Fe) is a trace metal element necessary for plant growth, but excess iron is harmful to plants. Natural resistance-associated macrophage proteins (NRAMPs) are important for divalent metal transport in plants. In this study, we isolated the MsNRAMP2 (MN_547960) gene from alfalfa, the perennial legume forage. The expression of MsNRAMP2 is specifically induced by iron excess. Overexpression of MsNRAMP2 conferred transgenic tobacco tolerance to iron excess, while it conferred yeast sensitivity to excess iron. Together with the MsNRAMP2 gene, MsMYB (MN_547959) expression is induced by excess iron. Y1H indicated that the MsMYB protein could bind to the “CTGTTG” cis element of the MsNRAMP2 promoter. The results indicated that MsNRAMP2 has a function in iron transport and its expression might be regulated by MsMYB. The excess iron tolerance ability enhancement of MsNRAMP2 may be involved in iron transport, sequestration, or redistribution.
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spelling pubmed-103799292023-07-29 MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter Li, Run-Tian Yang, Yun-Jiao Liu, Wen-Jun Liang, Wen-Wei Zhang, Miao Dong, Shi-Chen Shu, Yong-Jun Guo, Dong-Lin Guo, Chang-Hong Bi, Ying-Dong Int J Mol Sci Article Iron(Fe) is a trace metal element necessary for plant growth, but excess iron is harmful to plants. Natural resistance-associated macrophage proteins (NRAMPs) are important for divalent metal transport in plants. In this study, we isolated the MsNRAMP2 (MN_547960) gene from alfalfa, the perennial legume forage. The expression of MsNRAMP2 is specifically induced by iron excess. Overexpression of MsNRAMP2 conferred transgenic tobacco tolerance to iron excess, while it conferred yeast sensitivity to excess iron. Together with the MsNRAMP2 gene, MsMYB (MN_547959) expression is induced by excess iron. Y1H indicated that the MsMYB protein could bind to the “CTGTTG” cis element of the MsNRAMP2 promoter. The results indicated that MsNRAMP2 has a function in iron transport and its expression might be regulated by MsMYB. The excess iron tolerance ability enhancement of MsNRAMP2 may be involved in iron transport, sequestration, or redistribution. MDPI 2023-07-10 /pmc/articles/PMC10379929/ /pubmed/37511038 http://dx.doi.org/10.3390/ijms241411278 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Run-Tian
Yang, Yun-Jiao
Liu, Wen-Jun
Liang, Wen-Wei
Zhang, Miao
Dong, Shi-Chen
Shu, Yong-Jun
Guo, Dong-Lin
Guo, Chang-Hong
Bi, Ying-Dong
MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title_full MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title_fullStr MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title_full_unstemmed MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title_short MsNRAMP2 Enhances Tolerance to Iron Excess Stress in Nicotiana tabacum and MsMYB Binds to Its Promoter
title_sort msnramp2 enhances tolerance to iron excess stress in nicotiana tabacum and msmyb binds to its promoter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379929/
https://www.ncbi.nlm.nih.gov/pubmed/37511038
http://dx.doi.org/10.3390/ijms241411278
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