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Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model

(1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an AC...

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Autores principales: da Silva, Luana Laura Sales, Barbosa, Jéssica Anastácia Silva, João, Juliana Morelli Lopes Gonçalves, Fukuzaki, Silvia, Camargo, Leandro do Nascimento, dos Santos, Tabata Maruyama, de Campos, Elaine Cristina, Costa, Arthur Silva, Saraiva-Romanholo, Beatriz Mangueira, Bezerra, Suellen Karoline Moreira, Lopes, Fernanda Tenório Quirino dos Santos, Bonturi, Camila Ramalho, Oliva, Maria Luiza Vilela, Leick, Edna Aparecida, Righetti, Renato Fraga, Tibério, Iolanda de Fátima Lopes Calvo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379932/
https://www.ncbi.nlm.nih.gov/pubmed/37511021
http://dx.doi.org/10.3390/ijms241411261
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author da Silva, Luana Laura Sales
Barbosa, Jéssica Anastácia Silva
João, Juliana Morelli Lopes Gonçalves
Fukuzaki, Silvia
Camargo, Leandro do Nascimento
dos Santos, Tabata Maruyama
de Campos, Elaine Cristina
Costa, Arthur Silva
Saraiva-Romanholo, Beatriz Mangueira
Bezerra, Suellen Karoline Moreira
Lopes, Fernanda Tenório Quirino dos Santos
Bonturi, Camila Ramalho
Oliva, Maria Luiza Vilela
Leick, Edna Aparecida
Righetti, Renato Fraga
Tibério, Iolanda de Fátima Lopes Calvo
author_facet da Silva, Luana Laura Sales
Barbosa, Jéssica Anastácia Silva
João, Juliana Morelli Lopes Gonçalves
Fukuzaki, Silvia
Camargo, Leandro do Nascimento
dos Santos, Tabata Maruyama
de Campos, Elaine Cristina
Costa, Arthur Silva
Saraiva-Romanholo, Beatriz Mangueira
Bezerra, Suellen Karoline Moreira
Lopes, Fernanda Tenório Quirino dos Santos
Bonturi, Camila Ramalho
Oliva, Maria Luiza Vilela
Leick, Edna Aparecida
Righetti, Renato Fraga
Tibério, Iolanda de Fátima Lopes Calvo
author_sort da Silva, Luana Laura Sales
collection PubMed
description (1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ, TNF-α, MMP-9, MMP-12, TGF-β, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-κB in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-γ, TNF-α, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1β(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-γ, MMP-12 (AS), TGF-β (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model.
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spelling pubmed-103799322023-07-29 Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model da Silva, Luana Laura Sales Barbosa, Jéssica Anastácia Silva João, Juliana Morelli Lopes Gonçalves Fukuzaki, Silvia Camargo, Leandro do Nascimento dos Santos, Tabata Maruyama de Campos, Elaine Cristina Costa, Arthur Silva Saraiva-Romanholo, Beatriz Mangueira Bezerra, Suellen Karoline Moreira Lopes, Fernanda Tenório Quirino dos Santos Bonturi, Camila Ramalho Oliva, Maria Luiza Vilela Leick, Edna Aparecida Righetti, Renato Fraga Tibério, Iolanda de Fátima Lopes Calvo Int J Mol Sci Article (1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ, TNF-α, MMP-9, MMP-12, TGF-β, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-κB in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-γ, TNF-α, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1β(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-γ, MMP-12 (AS), TGF-β (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model. MDPI 2023-07-09 /pmc/articles/PMC10379932/ /pubmed/37511021 http://dx.doi.org/10.3390/ijms241411261 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Luana Laura Sales
Barbosa, Jéssica Anastácia Silva
João, Juliana Morelli Lopes Gonçalves
Fukuzaki, Silvia
Camargo, Leandro do Nascimento
dos Santos, Tabata Maruyama
de Campos, Elaine Cristina
Costa, Arthur Silva
Saraiva-Romanholo, Beatriz Mangueira
Bezerra, Suellen Karoline Moreira
Lopes, Fernanda Tenório Quirino dos Santos
Bonturi, Camila Ramalho
Oliva, Maria Luiza Vilela
Leick, Edna Aparecida
Righetti, Renato Fraga
Tibério, Iolanda de Fátima Lopes Calvo
Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title_full Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title_fullStr Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title_full_unstemmed Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title_short Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
title_sort effects of a peptide derived from the primary sequence of a kallikrein inhibitor isolated from bauhinia bauhinioides (pep-bbki) in an asthma–copd overlap (aco) model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379932/
https://www.ncbi.nlm.nih.gov/pubmed/37511021
http://dx.doi.org/10.3390/ijms241411261
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