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Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model
(1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an AC...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379932/ https://www.ncbi.nlm.nih.gov/pubmed/37511021 http://dx.doi.org/10.3390/ijms241411261 |
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author | da Silva, Luana Laura Sales Barbosa, Jéssica Anastácia Silva João, Juliana Morelli Lopes Gonçalves Fukuzaki, Silvia Camargo, Leandro do Nascimento dos Santos, Tabata Maruyama de Campos, Elaine Cristina Costa, Arthur Silva Saraiva-Romanholo, Beatriz Mangueira Bezerra, Suellen Karoline Moreira Lopes, Fernanda Tenório Quirino dos Santos Bonturi, Camila Ramalho Oliva, Maria Luiza Vilela Leick, Edna Aparecida Righetti, Renato Fraga Tibério, Iolanda de Fátima Lopes Calvo |
author_facet | da Silva, Luana Laura Sales Barbosa, Jéssica Anastácia Silva João, Juliana Morelli Lopes Gonçalves Fukuzaki, Silvia Camargo, Leandro do Nascimento dos Santos, Tabata Maruyama de Campos, Elaine Cristina Costa, Arthur Silva Saraiva-Romanholo, Beatriz Mangueira Bezerra, Suellen Karoline Moreira Lopes, Fernanda Tenório Quirino dos Santos Bonturi, Camila Ramalho Oliva, Maria Luiza Vilela Leick, Edna Aparecida Righetti, Renato Fraga Tibério, Iolanda de Fátima Lopes Calvo |
author_sort | da Silva, Luana Laura Sales |
collection | PubMed |
description | (1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ, TNF-α, MMP-9, MMP-12, TGF-β, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-κB in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-γ, TNF-α, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1β(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-γ, MMP-12 (AS), TGF-β (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model. |
format | Online Article Text |
id | pubmed-10379932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103799322023-07-29 Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model da Silva, Luana Laura Sales Barbosa, Jéssica Anastácia Silva João, Juliana Morelli Lopes Gonçalves Fukuzaki, Silvia Camargo, Leandro do Nascimento dos Santos, Tabata Maruyama de Campos, Elaine Cristina Costa, Arthur Silva Saraiva-Romanholo, Beatriz Mangueira Bezerra, Suellen Karoline Moreira Lopes, Fernanda Tenório Quirino dos Santos Bonturi, Camila Ramalho Oliva, Maria Luiza Vilela Leick, Edna Aparecida Righetti, Renato Fraga Tibério, Iolanda de Fátima Lopes Calvo Int J Mol Sci Article (1) There are several patients with asthma–COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ, TNF-α, MMP-9, MMP-12, TGF-β, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-κB in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-γ, TNF-α, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1β(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-γ, MMP-12 (AS), TGF-β (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model. MDPI 2023-07-09 /pmc/articles/PMC10379932/ /pubmed/37511021 http://dx.doi.org/10.3390/ijms241411261 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva, Luana Laura Sales Barbosa, Jéssica Anastácia Silva João, Juliana Morelli Lopes Gonçalves Fukuzaki, Silvia Camargo, Leandro do Nascimento dos Santos, Tabata Maruyama de Campos, Elaine Cristina Costa, Arthur Silva Saraiva-Romanholo, Beatriz Mangueira Bezerra, Suellen Karoline Moreira Lopes, Fernanda Tenório Quirino dos Santos Bonturi, Camila Ramalho Oliva, Maria Luiza Vilela Leick, Edna Aparecida Righetti, Renato Fraga Tibério, Iolanda de Fátima Lopes Calvo Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title | Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title_full | Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title_fullStr | Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title_full_unstemmed | Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title_short | Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma–COPD Overlap (ACO) Model |
title_sort | effects of a peptide derived from the primary sequence of a kallikrein inhibitor isolated from bauhinia bauhinioides (pep-bbki) in an asthma–copd overlap (aco) model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10379932/ https://www.ncbi.nlm.nih.gov/pubmed/37511021 http://dx.doi.org/10.3390/ijms241411261 |
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