Cargando…
A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli
Most broccoli cultivars or accessions exhibit green buds under appropriate growth conditions, which turn purple at cold temperatures. However, certain cultivars consistently maintain green buds both during normal growth and at cold temperatures. In this study, we used BSA-seq (bulked segregation ana...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380335/ https://www.ncbi.nlm.nih.gov/pubmed/37511150 http://dx.doi.org/10.3390/ijms241411391 |
_version_ | 1785080165826560000 |
---|---|
author | Yu, Huifang Wang, Jiansheng Shen, Yusen Sheng, Xiaoguang Shaw, Ranjan Kumar Branca, Ferdinando Gu, Honghui |
author_facet | Yu, Huifang Wang, Jiansheng Shen, Yusen Sheng, Xiaoguang Shaw, Ranjan Kumar Branca, Ferdinando Gu, Honghui |
author_sort | Yu, Huifang |
collection | PubMed |
description | Most broccoli cultivars or accessions exhibit green buds under appropriate growth conditions, which turn purple at cold temperatures. However, certain cultivars consistently maintain green buds both during normal growth and at cold temperatures. In this study, we used BSA-seq (bulked segregation analysis-sequencing), along with fine mapping and transcriptome analysis to identify a candidate gene (flavonoid 3′-hydroxylase, F3′H) responsible for reducing anthocyanin accumulation in the mutant GS and HX-16 broccoli (Brassica oleracea L. var. italica), which could retain green buds even at low temperatures. A 43-bp deletion was detected in the coding sequence (CDS) of the F3′H gene in HX-16 and the mutant GS, which significantly decreased F3′H expression and the accumulation of cyanidin and delphinidin in the mutant GS. Furthermore, the expression of F3′H was upregulated at low temperatures in the wild line PS. Our results demonstrated the efficacy of utilizing the 43-bp InDel (Insertion–Deletion) in predicting whether buds in B. oleracea L. will turn purple or remain green at cold temperatures across forty-two germplasm materials. This study provides critical genetic and molecular insights for the molecular breeding of B. oleracea and sheds light on the molecular mechanisms underlying the effect of low temperatures on bud color in broccoli. |
format | Online Article Text |
id | pubmed-10380335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103803352023-07-29 A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli Yu, Huifang Wang, Jiansheng Shen, Yusen Sheng, Xiaoguang Shaw, Ranjan Kumar Branca, Ferdinando Gu, Honghui Int J Mol Sci Article Most broccoli cultivars or accessions exhibit green buds under appropriate growth conditions, which turn purple at cold temperatures. However, certain cultivars consistently maintain green buds both during normal growth and at cold temperatures. In this study, we used BSA-seq (bulked segregation analysis-sequencing), along with fine mapping and transcriptome analysis to identify a candidate gene (flavonoid 3′-hydroxylase, F3′H) responsible for reducing anthocyanin accumulation in the mutant GS and HX-16 broccoli (Brassica oleracea L. var. italica), which could retain green buds even at low temperatures. A 43-bp deletion was detected in the coding sequence (CDS) of the F3′H gene in HX-16 and the mutant GS, which significantly decreased F3′H expression and the accumulation of cyanidin and delphinidin in the mutant GS. Furthermore, the expression of F3′H was upregulated at low temperatures in the wild line PS. Our results demonstrated the efficacy of utilizing the 43-bp InDel (Insertion–Deletion) in predicting whether buds in B. oleracea L. will turn purple or remain green at cold temperatures across forty-two germplasm materials. This study provides critical genetic and molecular insights for the molecular breeding of B. oleracea and sheds light on the molecular mechanisms underlying the effect of low temperatures on bud color in broccoli. MDPI 2023-07-13 /pmc/articles/PMC10380335/ /pubmed/37511150 http://dx.doi.org/10.3390/ijms241411391 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yu, Huifang Wang, Jiansheng Shen, Yusen Sheng, Xiaoguang Shaw, Ranjan Kumar Branca, Ferdinando Gu, Honghui A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title | A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title_full | A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title_fullStr | A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title_full_unstemmed | A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title_short | A 43 Bp-Deletion in the F3′H Gene Reducing Anthocyanins Is Responsible for Keeping Buds Green at Low Temperatures in Broccoli |
title_sort | 43 bp-deletion in the f3′h gene reducing anthocyanins is responsible for keeping buds green at low temperatures in broccoli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380335/ https://www.ncbi.nlm.nih.gov/pubmed/37511150 http://dx.doi.org/10.3390/ijms241411391 |
work_keys_str_mv | AT yuhuifang a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT wangjiansheng a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shenyusen a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shengxiaoguang a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shawranjankumar a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT brancaferdinando a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT guhonghui a43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT yuhuifang 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT wangjiansheng 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shenyusen 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shengxiaoguang 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT shawranjankumar 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT brancaferdinando 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli AT guhonghui 43bpdeletioninthef3hgenereducinganthocyaninsisresponsibleforkeepingbudsgreenatlowtemperaturesinbroccoli |