Cargando…
Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin
Tuberculosis (TB) is a zoonotic infectious disease caused by Mycobacterium tuberculosis (Mtb). Mtb is a typical intracellular parasite, and macrophages are its main host cells. NLRP3 inflammasome-mediated pyroptosis is a form of programmed cell death implicated in the clearance of pathogenic infecti...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380357/ https://www.ncbi.nlm.nih.gov/pubmed/37511451 http://dx.doi.org/10.3390/ijms241411692 |
_version_ | 1785080171714314240 |
---|---|
author | Nie, Xueyi Ma, Boli Liu, Lei Yuan, Xiaotan Li, Mengyuan Liu, Yueyang Hou, Yuxin Yang, Yi Xu, Jinrui Wang, Yujiong |
author_facet | Nie, Xueyi Ma, Boli Liu, Lei Yuan, Xiaotan Li, Mengyuan Liu, Yueyang Hou, Yuxin Yang, Yi Xu, Jinrui Wang, Yujiong |
author_sort | Nie, Xueyi |
collection | PubMed |
description | Tuberculosis (TB) is a zoonotic infectious disease caused by Mycobacterium tuberculosis (Mtb). Mtb is a typical intracellular parasite, and macrophages are its main host cells. NLRP3 inflammasome-mediated pyroptosis is a form of programmed cell death implicated in the clearance of pathogenic infections. The bidirectional regulatory effect of endoplasmic reticulum stress (ERS) plays a crucial role in determining cell survival and death. Whether ERS is involved in macrophage pyroptosis with Mtb infection remains unclear. This article aims to explore the regulation of the NLRP3 inflammasome and pyroptosis by ERS in THP-1 macrophages infected with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). The results showed that BCG infection induced THP-1 macrophage ERS, NLRP3 inflammasome activation and pyroptosis, which was inhibited by ERS inhibitor TUDCA. NLRP3 inhibitor MCC950 inhibited THP-1 macrophage NLRP3 inflammasome activation and pyroptosis caused by BCG infection. Compared with specific Caspase-1 inhibitor VX-765, pan-Caspase inhibitor Z-VAD-FMK showed a more significant inhibitory effect on BCG infection-induced pyroptosis of THP-1 macrophages. Taken together, this study demonstrates that ERS mediated NLRP3 inflammasome activation and pyroptosis after BCG infection of THP-1 macrophages, and that BCG infection of THP-1 macrophages induces pyroptosis through canonical and noncanonical pathways. |
format | Online Article Text |
id | pubmed-10380357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103803572023-07-29 Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin Nie, Xueyi Ma, Boli Liu, Lei Yuan, Xiaotan Li, Mengyuan Liu, Yueyang Hou, Yuxin Yang, Yi Xu, Jinrui Wang, Yujiong Int J Mol Sci Article Tuberculosis (TB) is a zoonotic infectious disease caused by Mycobacterium tuberculosis (Mtb). Mtb is a typical intracellular parasite, and macrophages are its main host cells. NLRP3 inflammasome-mediated pyroptosis is a form of programmed cell death implicated in the clearance of pathogenic infections. The bidirectional regulatory effect of endoplasmic reticulum stress (ERS) plays a crucial role in determining cell survival and death. Whether ERS is involved in macrophage pyroptosis with Mtb infection remains unclear. This article aims to explore the regulation of the NLRP3 inflammasome and pyroptosis by ERS in THP-1 macrophages infected with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). The results showed that BCG infection induced THP-1 macrophage ERS, NLRP3 inflammasome activation and pyroptosis, which was inhibited by ERS inhibitor TUDCA. NLRP3 inhibitor MCC950 inhibited THP-1 macrophage NLRP3 inflammasome activation and pyroptosis caused by BCG infection. Compared with specific Caspase-1 inhibitor VX-765, pan-Caspase inhibitor Z-VAD-FMK showed a more significant inhibitory effect on BCG infection-induced pyroptosis of THP-1 macrophages. Taken together, this study demonstrates that ERS mediated NLRP3 inflammasome activation and pyroptosis after BCG infection of THP-1 macrophages, and that BCG infection of THP-1 macrophages induces pyroptosis through canonical and noncanonical pathways. MDPI 2023-07-20 /pmc/articles/PMC10380357/ /pubmed/37511451 http://dx.doi.org/10.3390/ijms241411692 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nie, Xueyi Ma, Boli Liu, Lei Yuan, Xiaotan Li, Mengyuan Liu, Yueyang Hou, Yuxin Yang, Yi Xu, Jinrui Wang, Yujiong Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title | Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title_full | Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title_fullStr | Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title_full_unstemmed | Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title_short | Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Guérin |
title_sort | endoplasmic reticulum stress mediated nlrp3 inflammasome activation and pyroptosis in thp-1 macrophages infected with bacillus calmette-guérin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380357/ https://www.ncbi.nlm.nih.gov/pubmed/37511451 http://dx.doi.org/10.3390/ijms241411692 |
work_keys_str_mv | AT niexueyi endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT maboli endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT liulei endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT yuanxiaotan endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT limengyuan endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT liuyueyang endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT houyuxin endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT yangyi endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT xujinrui endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin AT wangyujiong endoplasmicreticulumstressmediatednlrp3inflammasomeactivationandpyroptosisinthp1macrophagesinfectedwithbacilluscalmetteguerin |