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MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway

MicroRNA (miRNA) is a non-coding RNA that can regulate the expression of many target genes, and it is widely involved in various important physiological activities. MiR-124-3p was found to associate with the normal development of retinal vessels in our previous study, but the mechanism of its anti-a...

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Autores principales: Hong, Yiwen, Wang, Yishen, Cui, Yamei, Pan, Jianying, Mao, Shudi, Zhu, Yanjie, Wen, Tao, Qi, Tianyuan, Wang, Aoxiang, Luo, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380620/
https://www.ncbi.nlm.nih.gov/pubmed/37511525
http://dx.doi.org/10.3390/ijms241411767
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author Hong, Yiwen
Wang, Yishen
Cui, Yamei
Pan, Jianying
Mao, Shudi
Zhu, Yanjie
Wen, Tao
Qi, Tianyuan
Wang, Aoxiang
Luo, Yan
author_facet Hong, Yiwen
Wang, Yishen
Cui, Yamei
Pan, Jianying
Mao, Shudi
Zhu, Yanjie
Wen, Tao
Qi, Tianyuan
Wang, Aoxiang
Luo, Yan
author_sort Hong, Yiwen
collection PubMed
description MicroRNA (miRNA) is a non-coding RNA that can regulate the expression of many target genes, and it is widely involved in various important physiological activities. MiR-124-3p was found to associate with the normal development of retinal vessels in our previous study, but the mechanism of its anti-angiogenic effect on pathological retinal neovascularization still needed to be explored. Therefore, this study aimed to investigate the effect and mechanism of miR-124-3p on retinal neovascularization in mice with oxygen-induced retinopathy (OIR). Here, we found that intravitreal injection of miR-124-3p agomir attenuated pathological retinal neovascularization in OIR mice. Moreover, miR-124-3p preserved the astrocytic template, inhibited reactive gliosis, and reduced the inflammatory response as well as necroptosis. Furthermore, miR-124-3p inhibited the signal transducer and activator of transcription 3 (STAT3) pathway and decreased the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor. Taken together, our results revealed that miR-124-3p inhibited retinal neovascularization and neuroglial dysfunction by targeting STAT3 in OIR mice.
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spelling pubmed-103806202023-07-29 MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway Hong, Yiwen Wang, Yishen Cui, Yamei Pan, Jianying Mao, Shudi Zhu, Yanjie Wen, Tao Qi, Tianyuan Wang, Aoxiang Luo, Yan Int J Mol Sci Article MicroRNA (miRNA) is a non-coding RNA that can regulate the expression of many target genes, and it is widely involved in various important physiological activities. MiR-124-3p was found to associate with the normal development of retinal vessels in our previous study, but the mechanism of its anti-angiogenic effect on pathological retinal neovascularization still needed to be explored. Therefore, this study aimed to investigate the effect and mechanism of miR-124-3p on retinal neovascularization in mice with oxygen-induced retinopathy (OIR). Here, we found that intravitreal injection of miR-124-3p agomir attenuated pathological retinal neovascularization in OIR mice. Moreover, miR-124-3p preserved the astrocytic template, inhibited reactive gliosis, and reduced the inflammatory response as well as necroptosis. Furthermore, miR-124-3p inhibited the signal transducer and activator of transcription 3 (STAT3) pathway and decreased the expression of hypoxia-inducible factor-1α and vascular endothelial growth factor. Taken together, our results revealed that miR-124-3p inhibited retinal neovascularization and neuroglial dysfunction by targeting STAT3 in OIR mice. MDPI 2023-07-21 /pmc/articles/PMC10380620/ /pubmed/37511525 http://dx.doi.org/10.3390/ijms241411767 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hong, Yiwen
Wang, Yishen
Cui, Yamei
Pan, Jianying
Mao, Shudi
Zhu, Yanjie
Wen, Tao
Qi, Tianyuan
Wang, Aoxiang
Luo, Yan
MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title_full MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title_fullStr MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title_full_unstemmed MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title_short MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway
title_sort microrna-124-3p attenuated retinal neovascularization in oxygen-induced retinopathy mice by inhibiting the dysfunction of retinal neuroglial cells through stat3 pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380620/
https://www.ncbi.nlm.nih.gov/pubmed/37511525
http://dx.doi.org/10.3390/ijms241411767
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