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Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis

Background: Emerging evidence suggests that long non-coding RNA (lncRNA) plays important roles in the regulation of gene expression. We determine the role of using urinary lncRNA as a non-invasive biomarker for lupus nephritis. Method: We studied three cohorts of lupus nephritis patients (31, 78, an...

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Autores principales: Szeto, Cheuk-Chun, So, Ho, Poon, Peter Yam-Kau, Luk, Cathy Choi-Wan, Ng, Jack Kit-Chung, Fung, Winston Wing-Shing, Chan, Gordon Chun-Kau, Chow, Kai-Ming, Lai, Fernand Mac-Moune, Tam, Lai-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380660/
https://www.ncbi.nlm.nih.gov/pubmed/37511572
http://dx.doi.org/10.3390/ijms241411813
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author Szeto, Cheuk-Chun
So, Ho
Poon, Peter Yam-Kau
Luk, Cathy Choi-Wan
Ng, Jack Kit-Chung
Fung, Winston Wing-Shing
Chan, Gordon Chun-Kau
Chow, Kai-Ming
Lai, Fernand Mac-Moune
Tam, Lai-Shan
author_facet Szeto, Cheuk-Chun
So, Ho
Poon, Peter Yam-Kau
Luk, Cathy Choi-Wan
Ng, Jack Kit-Chung
Fung, Winston Wing-Shing
Chan, Gordon Chun-Kau
Chow, Kai-Ming
Lai, Fernand Mac-Moune
Tam, Lai-Shan
author_sort Szeto, Cheuk-Chun
collection PubMed
description Background: Emerging evidence suggests that long non-coding RNA (lncRNA) plays important roles in the regulation of gene expression. We determine the role of using urinary lncRNA as a non-invasive biomarker for lupus nephritis. Method: We studied three cohorts of lupus nephritis patients (31, 78, and 12 patients, respectively) and controls (6, 7, and 24 subjects, respectively). The urinary sediment levels of specific lncRNA targets were studied using real-time quantitative polymerase chain reactions. Results: The severity of proteinuria inversely correlated with urinary maternally expressed gene 3 (MEG3) (r = −0.423, p = 0.018) and ANRIL levels (r = −0.483, p = 0.008). Urinary MEG3 level also inversely correlated with the SLEDAI score (r = −0.383, p = 0.034). Urinary cancer susceptibility candidate 2 (CASC2) levels were significantly different between histological classes of nephritis (p = 0.026) and patients with pure class V nephritis probably had the highest levels, while urinary metastasis-associated lung carcinoma transcript 1 (MALAT1) level significantly correlated with the histological activity index (r = −0.321, p = 0.004). Urinary taurine-upregulated gene 1 (TUG1) level was significantly lower in pure class V lupus nephritis than primary membranous nephropathy (p = 0.003) and minimal change nephropathy (p = 0.04), and urinary TUG1 level correlated with eGFR in class V lupus nephritis (r = 0.706, p = 0.01). Conclusions: We identified certain urinary lncRNA targets that may help the identification of lupus nephritis and predict the histological class of nephritis. Our findings indicate that urinary lncRNA levels may be developed as biomarkers for lupus nephritis.
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spelling pubmed-103806602023-07-29 Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis Szeto, Cheuk-Chun So, Ho Poon, Peter Yam-Kau Luk, Cathy Choi-Wan Ng, Jack Kit-Chung Fung, Winston Wing-Shing Chan, Gordon Chun-Kau Chow, Kai-Ming Lai, Fernand Mac-Moune Tam, Lai-Shan Int J Mol Sci Article Background: Emerging evidence suggests that long non-coding RNA (lncRNA) plays important roles in the regulation of gene expression. We determine the role of using urinary lncRNA as a non-invasive biomarker for lupus nephritis. Method: We studied three cohorts of lupus nephritis patients (31, 78, and 12 patients, respectively) and controls (6, 7, and 24 subjects, respectively). The urinary sediment levels of specific lncRNA targets were studied using real-time quantitative polymerase chain reactions. Results: The severity of proteinuria inversely correlated with urinary maternally expressed gene 3 (MEG3) (r = −0.423, p = 0.018) and ANRIL levels (r = −0.483, p = 0.008). Urinary MEG3 level also inversely correlated with the SLEDAI score (r = −0.383, p = 0.034). Urinary cancer susceptibility candidate 2 (CASC2) levels were significantly different between histological classes of nephritis (p = 0.026) and patients with pure class V nephritis probably had the highest levels, while urinary metastasis-associated lung carcinoma transcript 1 (MALAT1) level significantly correlated with the histological activity index (r = −0.321, p = 0.004). Urinary taurine-upregulated gene 1 (TUG1) level was significantly lower in pure class V lupus nephritis than primary membranous nephropathy (p = 0.003) and minimal change nephropathy (p = 0.04), and urinary TUG1 level correlated with eGFR in class V lupus nephritis (r = 0.706, p = 0.01). Conclusions: We identified certain urinary lncRNA targets that may help the identification of lupus nephritis and predict the histological class of nephritis. Our findings indicate that urinary lncRNA levels may be developed as biomarkers for lupus nephritis. MDPI 2023-07-22 /pmc/articles/PMC10380660/ /pubmed/37511572 http://dx.doi.org/10.3390/ijms241411813 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szeto, Cheuk-Chun
So, Ho
Poon, Peter Yam-Kau
Luk, Cathy Choi-Wan
Ng, Jack Kit-Chung
Fung, Winston Wing-Shing
Chan, Gordon Chun-Kau
Chow, Kai-Ming
Lai, Fernand Mac-Moune
Tam, Lai-Shan
Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title_full Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title_fullStr Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title_full_unstemmed Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title_short Urinary Long Non-Coding RNA Levels as Biomarkers of Lupus Nephritis
title_sort urinary long non-coding rna levels as biomarkers of lupus nephritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380660/
https://www.ncbi.nlm.nih.gov/pubmed/37511572
http://dx.doi.org/10.3390/ijms241411813
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