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miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients
Despite advances in non-small cell lung cancer (NSCLC) research, this is still the most common cancer type that has been diagnosed up to date. microRNAs have emerged as useful clinical biomarkers in both tissue and liquid biopsy. However, there are no reliable predictive biomarkers for clinical use....
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380700/ https://www.ncbi.nlm.nih.gov/pubmed/37511221 http://dx.doi.org/10.3390/ijms241411464 |
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author | Sanchez-Cabrero, Darío Garcia-Guede, Álvaro Burdiel, Miranda Pernía, Olga Colmenarejo-Fernandez, Julián Gutierrez, Laura Higuera, Oliver Rodriguez, Isabel Esteban Rosas-Alonso, Rocío Rodriguez-Antolín, Carlos Losantos-García, Itsaso Vera, Olga De Castro-Carpeño, Javier Ibanez de Caceres, Inmaculada |
author_facet | Sanchez-Cabrero, Darío Garcia-Guede, Álvaro Burdiel, Miranda Pernía, Olga Colmenarejo-Fernandez, Julián Gutierrez, Laura Higuera, Oliver Rodriguez, Isabel Esteban Rosas-Alonso, Rocío Rodriguez-Antolín, Carlos Losantos-García, Itsaso Vera, Olga De Castro-Carpeño, Javier Ibanez de Caceres, Inmaculada |
author_sort | Sanchez-Cabrero, Darío |
collection | PubMed |
description | Despite advances in non-small cell lung cancer (NSCLC) research, this is still the most common cancer type that has been diagnosed up to date. microRNAs have emerged as useful clinical biomarkers in both tissue and liquid biopsy. However, there are no reliable predictive biomarkers for clinical use. We evaluated the preclinical use of seven candidate miRNAs previously identified by our group. We collected a total of 120 prospective samples from 88 NSCLC patients. miRNA levels were analyzed via qRT-PCR from tissue and blood samples. miR-124 gene target prediction was performed using RNA sequencing data from our group and interrogating data from 2952 NSCLC patients from two public databases. We found higher levels of all seven miRNAs in tissue compared to plasma samples, except for miR-124. Our findings indicate that levels of miR-124, both free-circulating and within exosomes, are increased throughout the progression of the disease, suggesting its potential as a marker of disease progression in both advanced and early stages. Our bioinformatics approach identified KPNA4 and SPOCK1 as potential miR-124 targets in NSCLC. miR-124 levels can be used to identify early-stage NSCLC patients at higher risk of relapse. |
format | Online Article Text |
id | pubmed-10380700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103807002023-07-29 miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients Sanchez-Cabrero, Darío Garcia-Guede, Álvaro Burdiel, Miranda Pernía, Olga Colmenarejo-Fernandez, Julián Gutierrez, Laura Higuera, Oliver Rodriguez, Isabel Esteban Rosas-Alonso, Rocío Rodriguez-Antolín, Carlos Losantos-García, Itsaso Vera, Olga De Castro-Carpeño, Javier Ibanez de Caceres, Inmaculada Int J Mol Sci Article Despite advances in non-small cell lung cancer (NSCLC) research, this is still the most common cancer type that has been diagnosed up to date. microRNAs have emerged as useful clinical biomarkers in both tissue and liquid biopsy. However, there are no reliable predictive biomarkers for clinical use. We evaluated the preclinical use of seven candidate miRNAs previously identified by our group. We collected a total of 120 prospective samples from 88 NSCLC patients. miRNA levels were analyzed via qRT-PCR from tissue and blood samples. miR-124 gene target prediction was performed using RNA sequencing data from our group and interrogating data from 2952 NSCLC patients from two public databases. We found higher levels of all seven miRNAs in tissue compared to plasma samples, except for miR-124. Our findings indicate that levels of miR-124, both free-circulating and within exosomes, are increased throughout the progression of the disease, suggesting its potential as a marker of disease progression in both advanced and early stages. Our bioinformatics approach identified KPNA4 and SPOCK1 as potential miR-124 targets in NSCLC. miR-124 levels can be used to identify early-stage NSCLC patients at higher risk of relapse. MDPI 2023-07-14 /pmc/articles/PMC10380700/ /pubmed/37511221 http://dx.doi.org/10.3390/ijms241411464 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sanchez-Cabrero, Darío Garcia-Guede, Álvaro Burdiel, Miranda Pernía, Olga Colmenarejo-Fernandez, Julián Gutierrez, Laura Higuera, Oliver Rodriguez, Isabel Esteban Rosas-Alonso, Rocío Rodriguez-Antolín, Carlos Losantos-García, Itsaso Vera, Olga De Castro-Carpeño, Javier Ibanez de Caceres, Inmaculada miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title | miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title_full | miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title_fullStr | miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title_full_unstemmed | miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title_short | miR-124 as a Liquid Biopsy Prognostic Biomarker in Small Extracellular Vesicles from NSCLC Patients |
title_sort | mir-124 as a liquid biopsy prognostic biomarker in small extracellular vesicles from nsclc patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10380700/ https://www.ncbi.nlm.nih.gov/pubmed/37511221 http://dx.doi.org/10.3390/ijms241411464 |
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