Evaluation of the Attractant Effect of Solanum muricatum (Solanales: Solanaceae) on Gravid Female Adults of Zeugodacus tau (Diptera: Tephritidae) and Screening of Attractant Volatiles

SIMPLE SUMMARY: The Solanaceae crops are the main hosts of Zeugodacus tau, a pest insect that causes serious damage to economically important crops of Solanaceae. In the winter–spring period, female adults of Z. tau have been found to oviposit on the pepino melon Solanum muricatum. However, the differences in the ability of the fruits of S. muricatum and other Solanaceae crops to attract gravid Z. tau females have not been clarified. In this study, oviposition and trapping bioassays were performed to clarify whether such differences existed. GC-MS was used to identify the compounds and relative contents of the volatile odors of S. muricatum. The Y-tube olfactometer system was used to determine the compounds that induced behavioral responses in gravid Z. tau females. The results show that Solanum muricatum odor was attractive for Z. tau females. The nine compounds from the volatiles of S. muricatum induce behavioral responses in gravid Z. tau females. These results are beneficial for preventing Z. tau females from harming S. muricatum, developing attractants and repellents for Z. tau females, and establishing a system of ecological control for Z. tau females. ABSTRACT: Zeugodacus tau (Walker) (Diptera: Tephritidae) is a pest seriously harmful to Solanaceae crops and was found to oviposit on the pepino melon Solanum muricatum (Aiton). To date, the differences in the ability of the fruits of S. muricatum and other Solanaceae crops to attract gravid Z. tau females have seldom been reported. Oviposition and trapping bioassays were performed to clarify whether such differences existed. A combination of GC-MS and the Y-tube olfactometer system was used to identify and determine the compounds inducing behavioral responses in gravid Z. tau females to the volatile odors of S. muricatum. The results show that S. muricatum odors play a role in attracting gravid Z. tau females. The odors of Solanaceae crops influence their ability to attract these organisms. The nine compounds from the volatiles of S. muricatum induce tendency or repellency responses in gravid Z. tau females. Hexyl acetate, butyl acetate, amyl actate, and isoamyl acetate caused tendency behavior in gravid Z. tau females, while hexyl hexanoate, butyl isovalerate, butyl valerate, and isoamyl hexanoate caused repellency behavior. Heptyl acetate caused repellency behavior in gravid Z. tau females at higher concentrations (5 mg/mL) but caused tendency behavior at a low concentration (0.5 mg/mL). These results suggest that vigilance against the harm caused by Z. tau is required during the cultivation of S. muricatum. The nine compounds of the volatile odors of S. muricatum could help to develop attractants and repellents for gravid Z. tau females. These results are beneficial for preventing Z. tau females from harming S. muricatum, developing attractants and repellents for Z. tau females, and establishing a system of ecological control for Z. tau females.