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Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice

Alzheimer’s disease (AD) is characterized by progressive cognitive decline and is a leading cause of death in the United States. Neuroinflammation has been implicated in the progression of AD, and several recent studies suggest that peripheral immune dysfunction may influence the disease. Continuing...

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Autores principales: Sahu, Bijayani, Johnson, Lauren M., Sohrabi, Mona, Usatii, Anastasia A., Craig, Rachel M. J., Kaelberer, Joshua B., Chandrasekaran, Sathiya Priya, Kaur, Harpreet, Nookala, Suba, Combs, Colin K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381012/
https://www.ncbi.nlm.nih.gov/pubmed/37511312
http://dx.doi.org/10.3390/ijms241411551
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author Sahu, Bijayani
Johnson, Lauren M.
Sohrabi, Mona
Usatii, Anastasia A.
Craig, Rachel M. J.
Kaelberer, Joshua B.
Chandrasekaran, Sathiya Priya
Kaur, Harpreet
Nookala, Suba
Combs, Colin K.
author_facet Sahu, Bijayani
Johnson, Lauren M.
Sohrabi, Mona
Usatii, Anastasia A.
Craig, Rachel M. J.
Kaelberer, Joshua B.
Chandrasekaran, Sathiya Priya
Kaur, Harpreet
Nookala, Suba
Combs, Colin K.
author_sort Sahu, Bijayani
collection PubMed
description Alzheimer’s disease (AD) is characterized by progressive cognitive decline and is a leading cause of death in the United States. Neuroinflammation has been implicated in the progression of AD, and several recent studies suggest that peripheral immune dysfunction may influence the disease. Continuing evidence indicates that intestinal dysbiosis is an attribute of AD, and inflammatory bowel disease (IBD) has been shown to aggravate cognitive impairment. Previously, we separately demonstrated that an IBD-like condition exacerbates AD-related changes in the brains of the App(NL-G-F) mouse model of AD, while probiotic intervention has an attenuating effect. In this study, we investigated the combination of a dietary probiotic and an IBD-like condition for effects on the brains of mice. Male C57BL/6 wild type (WT) and App(NL-G-F) mice were randomly divided into four groups: vehicle control, oral probiotic, dextran sulfate sodium (DSS), and DSS given with probiotics. As anticipated, probiotic treatment attenuated the DSS-induced colitis disease activity index in WT and App(NL-G-F) mice. Although probiotic feeding significantly attenuated the DSS-mediated increase in WT colonic lipocalin levels, it was less protective in the App(NL-G-F) DSS-treated group. In parallel with the intestinal changes, combined probiotic and DSS treatment increased microglial, neutrophil elastase, and 5hmC immunoreactivity while decreasing c-Fos staining compared to DSS treatment alone in the brains of WT mice. Although less abundant, probiotic combined with DSS treatment demonstrated a few similar changes in App(NL-G-F) brains with increased microglial and decreased c-Fos immunoreactivity in addition to a slight increase in Aβ plaque staining. Both probiotic and DSS treatment also altered the levels of several cytokines in WT and App(NL-G-F) brains, with a unique increase in the levels of TNFα and IL-2 being observed in only App(NL-G-F) mice following combined DSS and probiotic treatment. Our data indicate that, while dietary probiotic intervention provides protection against the colitis-like condition, it also influences numerous glial, cytokine, and neuronal changes in the brain that may regulate brain function and the progression of AD.
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spelling pubmed-103810122023-07-29 Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice Sahu, Bijayani Johnson, Lauren M. Sohrabi, Mona Usatii, Anastasia A. Craig, Rachel M. J. Kaelberer, Joshua B. Chandrasekaran, Sathiya Priya Kaur, Harpreet Nookala, Suba Combs, Colin K. Int J Mol Sci Article Alzheimer’s disease (AD) is characterized by progressive cognitive decline and is a leading cause of death in the United States. Neuroinflammation has been implicated in the progression of AD, and several recent studies suggest that peripheral immune dysfunction may influence the disease. Continuing evidence indicates that intestinal dysbiosis is an attribute of AD, and inflammatory bowel disease (IBD) has been shown to aggravate cognitive impairment. Previously, we separately demonstrated that an IBD-like condition exacerbates AD-related changes in the brains of the App(NL-G-F) mouse model of AD, while probiotic intervention has an attenuating effect. In this study, we investigated the combination of a dietary probiotic and an IBD-like condition for effects on the brains of mice. Male C57BL/6 wild type (WT) and App(NL-G-F) mice were randomly divided into four groups: vehicle control, oral probiotic, dextran sulfate sodium (DSS), and DSS given with probiotics. As anticipated, probiotic treatment attenuated the DSS-induced colitis disease activity index in WT and App(NL-G-F) mice. Although probiotic feeding significantly attenuated the DSS-mediated increase in WT colonic lipocalin levels, it was less protective in the App(NL-G-F) DSS-treated group. In parallel with the intestinal changes, combined probiotic and DSS treatment increased microglial, neutrophil elastase, and 5hmC immunoreactivity while decreasing c-Fos staining compared to DSS treatment alone in the brains of WT mice. Although less abundant, probiotic combined with DSS treatment demonstrated a few similar changes in App(NL-G-F) brains with increased microglial and decreased c-Fos immunoreactivity in addition to a slight increase in Aβ plaque staining. Both probiotic and DSS treatment also altered the levels of several cytokines in WT and App(NL-G-F) brains, with a unique increase in the levels of TNFα and IL-2 being observed in only App(NL-G-F) mice following combined DSS and probiotic treatment. Our data indicate that, while dietary probiotic intervention provides protection against the colitis-like condition, it also influences numerous glial, cytokine, and neuronal changes in the brain that may regulate brain function and the progression of AD. MDPI 2023-07-17 /pmc/articles/PMC10381012/ /pubmed/37511312 http://dx.doi.org/10.3390/ijms241411551 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sahu, Bijayani
Johnson, Lauren M.
Sohrabi, Mona
Usatii, Anastasia A.
Craig, Rachel M. J.
Kaelberer, Joshua B.
Chandrasekaran, Sathiya Priya
Kaur, Harpreet
Nookala, Suba
Combs, Colin K.
Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title_full Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title_fullStr Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title_full_unstemmed Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title_short Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in App(NL-G-F) Mice
title_sort effects of probiotics on colitis-induced exacerbation of alzheimer’s disease in app(nl-g-f) mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381012/
https://www.ncbi.nlm.nih.gov/pubmed/37511312
http://dx.doi.org/10.3390/ijms241411551
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