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Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice

Dietary fat can alter host metabolism and gut microbial composition. Crocodile oil (CO) was extracted from the fatty tissues of Crocodylus siamensis. CO, rich in monounsaturated- and polyunsaturated fatty acids, has been reported to reduce inflammation, counter toxification, and improve energy metab...

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Autores principales: Parunyakul, Kongphop, Chuchoiy, Aphisara, Kooltueon, Sasiporn, Puttagamnerd, Phiyaporn, Srisuksai, Krittika, Santativongchai, Pitchaya, Pongchairerk, Urai, Tulayakul, Phitsanu, E-kobon, Teerasak, Fungfuang, Wirasak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381048/
https://www.ncbi.nlm.nih.gov/pubmed/37506097
http://dx.doi.org/10.1371/journal.pone.0289073
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author Parunyakul, Kongphop
Chuchoiy, Aphisara
Kooltueon, Sasiporn
Puttagamnerd, Phiyaporn
Srisuksai, Krittika
Santativongchai, Pitchaya
Pongchairerk, Urai
Tulayakul, Phitsanu
E-kobon, Teerasak
Fungfuang, Wirasak
author_facet Parunyakul, Kongphop
Chuchoiy, Aphisara
Kooltueon, Sasiporn
Puttagamnerd, Phiyaporn
Srisuksai, Krittika
Santativongchai, Pitchaya
Pongchairerk, Urai
Tulayakul, Phitsanu
E-kobon, Teerasak
Fungfuang, Wirasak
author_sort Parunyakul, Kongphop
collection PubMed
description Dietary fat can alter host metabolism and gut microbial composition. Crocodile oil (CO) was extracted from the fatty tissues of Crocodylus siamensis. CO, rich in monounsaturated- and polyunsaturated fatty acids, has been reported to reduce inflammation, counter toxification, and improve energy metabolism. The aim of this study was to investigate the effect of CO on gut microbiota (GM) in laboratory mice as well as the accompanying metabolic changes in the animals. Forty-five C57BL/6 male mice were randomly divided into five groups and orally administrated either sterile water (control [C]); 1 or 3% (v/w) CO (CO-low [CO-L] and CO-high [CO-H], respectively); or 1 or 3% (v/w) palm oil (PO-low and PO-high, respectively) for 11 weeks. Body weight gain, food intake, energy intake, blood glucose levels, and blood lipid profiles were determined. Samples from colon tissue were collected and the 16S rRNA genes were pyrosequenced to clarify GM analyses. The results showed that there were no differences in body weight and blood glucose levels. Food intake by the mice in the CO-L and CO-H groups was statistically significantly less when compared to that by the animals in the C group. However, neither CO treatment had a statistically significant effect on calorie intake when compared to the controls. The CO-H exhibited a significant increase in serum total cholesterol and low-density lipoprotein but showed a downward trend in triglyceride levels compared to the control. The GM analyses revealed that both CO treatments have no significant influence on bacterial diversity and relative abundance at the phylum level, whereas increases of Choa1 and abundance-based coverage estimator indexes, distinct β-diversity, and Proteobacteria abundance were observed in the PO-high group compared with the C group. Furthermore, the abundance of Azospirillum thiophilum and Romboutsia ilealis was significantly higher in the CO-L and CO-H groups which could be associated with energy metabolic activity. Thus, CO may be an alternative fat source for preserving host metabolism and gut flora.
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spelling pubmed-103810482023-07-29 Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice Parunyakul, Kongphop Chuchoiy, Aphisara Kooltueon, Sasiporn Puttagamnerd, Phiyaporn Srisuksai, Krittika Santativongchai, Pitchaya Pongchairerk, Urai Tulayakul, Phitsanu E-kobon, Teerasak Fungfuang, Wirasak PLoS One Research Article Dietary fat can alter host metabolism and gut microbial composition. Crocodile oil (CO) was extracted from the fatty tissues of Crocodylus siamensis. CO, rich in monounsaturated- and polyunsaturated fatty acids, has been reported to reduce inflammation, counter toxification, and improve energy metabolism. The aim of this study was to investigate the effect of CO on gut microbiota (GM) in laboratory mice as well as the accompanying metabolic changes in the animals. Forty-five C57BL/6 male mice were randomly divided into five groups and orally administrated either sterile water (control [C]); 1 or 3% (v/w) CO (CO-low [CO-L] and CO-high [CO-H], respectively); or 1 or 3% (v/w) palm oil (PO-low and PO-high, respectively) for 11 weeks. Body weight gain, food intake, energy intake, blood glucose levels, and blood lipid profiles were determined. Samples from colon tissue were collected and the 16S rRNA genes were pyrosequenced to clarify GM analyses. The results showed that there were no differences in body weight and blood glucose levels. Food intake by the mice in the CO-L and CO-H groups was statistically significantly less when compared to that by the animals in the C group. However, neither CO treatment had a statistically significant effect on calorie intake when compared to the controls. The CO-H exhibited a significant increase in serum total cholesterol and low-density lipoprotein but showed a downward trend in triglyceride levels compared to the control. The GM analyses revealed that both CO treatments have no significant influence on bacterial diversity and relative abundance at the phylum level, whereas increases of Choa1 and abundance-based coverage estimator indexes, distinct β-diversity, and Proteobacteria abundance were observed in the PO-high group compared with the C group. Furthermore, the abundance of Azospirillum thiophilum and Romboutsia ilealis was significantly higher in the CO-L and CO-H groups which could be associated with energy metabolic activity. Thus, CO may be an alternative fat source for preserving host metabolism and gut flora. Public Library of Science 2023-07-28 /pmc/articles/PMC10381048/ /pubmed/37506097 http://dx.doi.org/10.1371/journal.pone.0289073 Text en © 2023 Parunyakul et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Parunyakul, Kongphop
Chuchoiy, Aphisara
Kooltueon, Sasiporn
Puttagamnerd, Phiyaporn
Srisuksai, Krittika
Santativongchai, Pitchaya
Pongchairerk, Urai
Tulayakul, Phitsanu
E-kobon, Teerasak
Fungfuang, Wirasak
Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title_full Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title_fullStr Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title_full_unstemmed Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title_short Effect of the oil from the fatty tissues of Crocodylus siamensis on gut microbiome diversity and metabolism in mice
title_sort effect of the oil from the fatty tissues of crocodylus siamensis on gut microbiome diversity and metabolism in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381048/
https://www.ncbi.nlm.nih.gov/pubmed/37506097
http://dx.doi.org/10.1371/journal.pone.0289073
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