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Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest
Multiresistant pathogens pose a serious threat to human health. The genus Candida is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381380/ https://www.ncbi.nlm.nih.gov/pubmed/37504744 http://dx.doi.org/10.3390/jof9070756 |
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author | Carvalho, Rayara J. P. Souza, Pedro F. N. Malveira, Ellen A. Neto, Nilton A. S. Silva, Romério R. S. Melo, Gabriel L. C. Silva, Ayrles F. B. Lima, Leandro B. de Albuquerque, Cynthia C. Bastos, Rafael W. Goldman, Gustavo H. de Freitas, Cleverson D. T. |
author_facet | Carvalho, Rayara J. P. Souza, Pedro F. N. Malveira, Ellen A. Neto, Nilton A. S. Silva, Romério R. S. Melo, Gabriel L. C. Silva, Ayrles F. B. Lima, Leandro B. de Albuquerque, Cynthia C. Bastos, Rafael W. Goldman, Gustavo H. de Freitas, Cleverson D. T. |
author_sort | Carvalho, Rayara J. P. |
collection | PubMed |
description | Multiresistant pathogens pose a serious threat to human health. The genus Candida is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens. Based on that, the present study aimed to evaluate the antimicrobial potential of essential oil from C. pluriglandulosus and understand the mechanism of action. Here, it highlighted antimicrobial activity and the mechanisms of action of the essential oil extracted from C. pluriglandulosus Carn.-Torres & Riina (CpEO) leaves on human pathogenic microorganisms in planktonic and biofilm lifestyles. In addition, for the first time, the oil composition was revealed by GC-MS analysis and the toxicity to human red blood cells (HRBC). Twenty-six chemical compounds were identified in CpEO, elemicin, bicyclogermacrene, caryophyllene, brevifolin, and 2,4,6-trimethoxy-styrene. Through hemolytic assay, it was shown that CpEO has no toxicity to human RBCs. At the concentration of 50 μg mL(−1), CpEO did not show great antibacterial potential. However, promising data were found for C. krusei and C. parapsilosis inhibiting by 89.3% and 80.7% of planktonic cell growth and 83.5% and 77.9% the biofilm formation, respectively. Furthermore, the mechanisms of action CpEO were elucidated by fluorescence. Scanning electron microscopy revealed damage to the cell membrane and pore formation, ROS overproduction, and induction of apoptosis in candida cells. Our results reinforce the potential of CpEO as an effective alternative molecule of pharmaceutical interest. |
format | Online Article Text |
id | pubmed-10381380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103813802023-07-29 Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest Carvalho, Rayara J. P. Souza, Pedro F. N. Malveira, Ellen A. Neto, Nilton A. S. Silva, Romério R. S. Melo, Gabriel L. C. Silva, Ayrles F. B. Lima, Leandro B. de Albuquerque, Cynthia C. Bastos, Rafael W. Goldman, Gustavo H. de Freitas, Cleverson D. T. J Fungi (Basel) Article Multiresistant pathogens pose a serious threat to human health. The genus Candida is one class of human pathogenic yeasts responsible for infections affecting healthy and immunocompromised patients. In this context, plant essential oils emerged as a future natural alternative to control the diseases caused by these pathogens. Based on that, the present study aimed to evaluate the antimicrobial potential of essential oil from C. pluriglandulosus and understand the mechanism of action. Here, it highlighted antimicrobial activity and the mechanisms of action of the essential oil extracted from C. pluriglandulosus Carn.-Torres & Riina (CpEO) leaves on human pathogenic microorganisms in planktonic and biofilm lifestyles. In addition, for the first time, the oil composition was revealed by GC-MS analysis and the toxicity to human red blood cells (HRBC). Twenty-six chemical compounds were identified in CpEO, elemicin, bicyclogermacrene, caryophyllene, brevifolin, and 2,4,6-trimethoxy-styrene. Through hemolytic assay, it was shown that CpEO has no toxicity to human RBCs. At the concentration of 50 μg mL(−1), CpEO did not show great antibacterial potential. However, promising data were found for C. krusei and C. parapsilosis inhibiting by 89.3% and 80.7% of planktonic cell growth and 83.5% and 77.9% the biofilm formation, respectively. Furthermore, the mechanisms of action CpEO were elucidated by fluorescence. Scanning electron microscopy revealed damage to the cell membrane and pore formation, ROS overproduction, and induction of apoptosis in candida cells. Our results reinforce the potential of CpEO as an effective alternative molecule of pharmaceutical interest. MDPI 2023-07-17 /pmc/articles/PMC10381380/ /pubmed/37504744 http://dx.doi.org/10.3390/jof9070756 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Carvalho, Rayara J. P. Souza, Pedro F. N. Malveira, Ellen A. Neto, Nilton A. S. Silva, Romério R. S. Melo, Gabriel L. C. Silva, Ayrles F. B. Lima, Leandro B. de Albuquerque, Cynthia C. Bastos, Rafael W. Goldman, Gustavo H. de Freitas, Cleverson D. T. Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title | Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title_full | Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title_fullStr | Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title_full_unstemmed | Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title_short | Antimicrobial Activity the Essential Oil from Croton pluriglandulosus Carn. Leaves against Microorganisms of Clinical Interest |
title_sort | antimicrobial activity the essential oil from croton pluriglandulosus carn. leaves against microorganisms of clinical interest |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381380/ https://www.ncbi.nlm.nih.gov/pubmed/37504744 http://dx.doi.org/10.3390/jof9070756 |
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