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Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid

Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA usi...

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Autores principales: Sulej, Justyna, Piątek-Gołda, Wiktoria, Grąz, Marcin, Szałapata, Katarzyna, Waśko, Piotr, Janik-Zabrotowicz, Ewa, Osińska-Jaroszuk, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381469/
https://www.ncbi.nlm.nih.gov/pubmed/37504878
http://dx.doi.org/10.3390/jfb14070383
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author Sulej, Justyna
Piątek-Gołda, Wiktoria
Grąz, Marcin
Szałapata, Katarzyna
Waśko, Piotr
Janik-Zabrotowicz, Ewa
Osińska-Jaroszuk, Monika
author_facet Sulej, Justyna
Piątek-Gołda, Wiktoria
Grąz, Marcin
Szałapata, Katarzyna
Waśko, Piotr
Janik-Zabrotowicz, Ewa
Osińska-Jaroszuk, Monika
author_sort Sulej, Justyna
collection PubMed
description Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications.
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spelling pubmed-103814692023-07-29 Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid Sulej, Justyna Piątek-Gołda, Wiktoria Grąz, Marcin Szałapata, Katarzyna Waśko, Piotr Janik-Zabrotowicz, Ewa Osińska-Jaroszuk, Monika J Funct Biomater Article Lactobionic acid (LBA) is a bioactive compound that has become increasingly popular in medicine in recent years due to its unique properties. This chemical can be formed via the enzymatic oxidation of lactose using fungal oxidoreductive enzymes. This study aimed to intensify the synthesis of LBA using immobilised enzymes (cellobiose dehydrogenase from Phanerochaete chrysosporium (PchCDH) and laccase from Cerrena unicolor (CuLAC)) on chitosan microspheres. We used three different crosslinking agents: genipin, glutaraldehyde, and polyethyleneimine to activate the chitosan. The FTIR and CellDrop techniques were used to characterise the activated microspheres. Quantitative (HPLC) and qualitative (TLC) methods were used to determine the obtained LBA. The results show that the type of activator used influences the efficiency of the binding of the enzyme to the matrix. Furthermore, the amount of LBA formed depends on the type of system used. The use of a system in which one of the enzymes is immobilised on a PEI-activated carrier (PchCDH) and the other is free (CuLAC) proved to be the most optimal, as it yielded almost 100% conversion of lactose to lactobionic acid. Summarising the data obtained the following: lactobionic acid immobilised on chitosan microspheres has great potential for medical applications. MDPI 2023-07-21 /pmc/articles/PMC10381469/ /pubmed/37504878 http://dx.doi.org/10.3390/jfb14070383 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sulej, Justyna
Piątek-Gołda, Wiktoria
Grąz, Marcin
Szałapata, Katarzyna
Waśko, Piotr
Janik-Zabrotowicz, Ewa
Osińska-Jaroszuk, Monika
Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title_full Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title_fullStr Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title_full_unstemmed Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title_short Immobilisation of Cellobiose Dehydrogenase and Laccase on Chitosan Particles as a Multi-Enzymatic System for the Synthesis of Lactobionic Acid
title_sort immobilisation of cellobiose dehydrogenase and laccase on chitosan particles as a multi-enzymatic system for the synthesis of lactobionic acid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381469/
https://www.ncbi.nlm.nih.gov/pubmed/37504878
http://dx.doi.org/10.3390/jfb14070383
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