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Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas
Intraductal papillary mucinous neoplasm (IPMN) is the most common pancreatic cyst and a precursor of pancreatic cancer (PDAC). Since PDAC has a devastatingly high mortality rate, the early diagnosis and treatment of any precursor lesion are rational. The safety of the existing guidelines on the clin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381561/ https://www.ncbi.nlm.nih.gov/pubmed/37511945 http://dx.doi.org/10.3390/life13071570 |
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author | Kuvendjiska, Jasmina Müller, Felix Bronsert, Peter Timme-Bronsert, Sylvia Fichtner-Feigl, Stefan Kulemann, Birte |
author_facet | Kuvendjiska, Jasmina Müller, Felix Bronsert, Peter Timme-Bronsert, Sylvia Fichtner-Feigl, Stefan Kulemann, Birte |
author_sort | Kuvendjiska, Jasmina |
collection | PubMed |
description | Intraductal papillary mucinous neoplasm (IPMN) is the most common pancreatic cyst and a precursor of pancreatic cancer (PDAC). Since PDAC has a devastatingly high mortality rate, the early diagnosis and treatment of any precursor lesion are rational. The safety of the existing guidelines on the clinical management of IPMN has been criticized due to unsatisfactory sensitivity and specificity, showing the need for further markers. Blood obtained from patients with IPMN was therefore subjected to size-based isolation of circulating epithelial cells (CECs). We isolated CECs and evaluated their cytological characteristics. Additionally, we compared Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations in CECs and the primary IPMN tissue, since KRAS mutations are very typical for PDAC. Samples from 27 IPMN patients were analyzed. In 10 (37%) patients, CECs were isolated and showed a hybrid pattern of surface markers involving both epithelial and mesenchymal markers, suggesting a possible EMT process of the cells. Especially, patients with high-grade dysplasia in the main specimen were all CEC-positive. KRAS mutations were also present in CECs but less common than in IPMN tissue. The existence of CEC in IPMN patients offers additional blood-based research possibilities for IMPN biology. |
format | Online Article Text |
id | pubmed-10381561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103815612023-07-29 Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas Kuvendjiska, Jasmina Müller, Felix Bronsert, Peter Timme-Bronsert, Sylvia Fichtner-Feigl, Stefan Kulemann, Birte Life (Basel) Article Intraductal papillary mucinous neoplasm (IPMN) is the most common pancreatic cyst and a precursor of pancreatic cancer (PDAC). Since PDAC has a devastatingly high mortality rate, the early diagnosis and treatment of any precursor lesion are rational. The safety of the existing guidelines on the clinical management of IPMN has been criticized due to unsatisfactory sensitivity and specificity, showing the need for further markers. Blood obtained from patients with IPMN was therefore subjected to size-based isolation of circulating epithelial cells (CECs). We isolated CECs and evaluated their cytological characteristics. Additionally, we compared Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations in CECs and the primary IPMN tissue, since KRAS mutations are very typical for PDAC. Samples from 27 IPMN patients were analyzed. In 10 (37%) patients, CECs were isolated and showed a hybrid pattern of surface markers involving both epithelial and mesenchymal markers, suggesting a possible EMT process of the cells. Especially, patients with high-grade dysplasia in the main specimen were all CEC-positive. KRAS mutations were also present in CECs but less common than in IPMN tissue. The existence of CEC in IPMN patients offers additional blood-based research possibilities for IMPN biology. MDPI 2023-07-15 /pmc/articles/PMC10381561/ /pubmed/37511945 http://dx.doi.org/10.3390/life13071570 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kuvendjiska, Jasmina Müller, Felix Bronsert, Peter Timme-Bronsert, Sylvia Fichtner-Feigl, Stefan Kulemann, Birte Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title | Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title_full | Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title_fullStr | Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title_full_unstemmed | Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title_short | Circulating Epithelial Cells in Patients with Intraductal Papillary Mucinous Neoplasm of the Pancreas |
title_sort | circulating epithelial cells in patients with intraductal papillary mucinous neoplasm of the pancreas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381561/ https://www.ncbi.nlm.nih.gov/pubmed/37511945 http://dx.doi.org/10.3390/life13071570 |
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