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The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples

Background: Candida spp., as part of the microbiota, can colonise the gastrointestinal tract. We hypothesised that genotyping Candida spp. isolates from the gastrointestinal tract could help spot genotypes able to cause invasive infections. Materials/methods: A total of 816 isolates of C. albicans (...

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Autores principales: Mesquida, Aina, Machado, Marina, Dávila-Cherres, Lorena, Vicente, Teresa, Sánchez-Carrillo, Carlos, Alcalá, Luis, Reigadas, Elena, Muñoz, Patricia, Guinea, Jesús, Escribano, Pilar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381600/
https://www.ncbi.nlm.nih.gov/pubmed/37504721
http://dx.doi.org/10.3390/jof9070732
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author Mesquida, Aina
Machado, Marina
Dávila-Cherres, Lorena
Vicente, Teresa
Sánchez-Carrillo, Carlos
Alcalá, Luis
Reigadas, Elena
Muñoz, Patricia
Guinea, Jesús
Escribano, Pilar
author_facet Mesquida, Aina
Machado, Marina
Dávila-Cherres, Lorena
Vicente, Teresa
Sánchez-Carrillo, Carlos
Alcalá, Luis
Reigadas, Elena
Muñoz, Patricia
Guinea, Jesús
Escribano, Pilar
author_sort Mesquida, Aina
collection PubMed
description Background: Candida spp., as part of the microbiota, can colonise the gastrointestinal tract. We hypothesised that genotyping Candida spp. isolates from the gastrointestinal tract could help spot genotypes able to cause invasive infections. Materials/methods: A total of 816 isolates of C. albicans (n = 595), C. parapsilosis (n = 118), and C. tropicalis (n = 103) from rectal swabs (n = 754 patients) were studied. Genotyping was conducted using species-specific microsatellite markers. Rectal swab genotypes were compared with previously studied blood (n = 814) and intra-abdominal (n = 202) genotypes. Results: A total of 36/754 patients had the same Candida spp. isolated from blood cultures, intra-abdominal samples, or both; these patients had candidemia (n = 18), intra-abdominal candidiasis (n = 11), both clinical forms (n = 1), and non-significant isolation (n = 6). Genotypes matching the rectal swab and their blood cultures (84.2%) or their intra-abdominal samples (92.3%) were found in most of the significant patients. We detected 656 genotypes from rectal swabs, 88.4% of which were singletons and 11.6% were clusters. Of these 656 rectal swab genotypes, 94 (14.3%) were also detected in blood cultures and 34 (5.2%) in intra-abdominal samples. Of the rectal swab clusters, 62.7% were previously defined as a widespread genotype. Conclusions: Our study pinpoints the gastrointestinal tract as a potential reservoir of potentially invasive Candida spp. genotypes.
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spelling pubmed-103816002023-07-29 The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples Mesquida, Aina Machado, Marina Dávila-Cherres, Lorena Vicente, Teresa Sánchez-Carrillo, Carlos Alcalá, Luis Reigadas, Elena Muñoz, Patricia Guinea, Jesús Escribano, Pilar J Fungi (Basel) Communication Background: Candida spp., as part of the microbiota, can colonise the gastrointestinal tract. We hypothesised that genotyping Candida spp. isolates from the gastrointestinal tract could help spot genotypes able to cause invasive infections. Materials/methods: A total of 816 isolates of C. albicans (n = 595), C. parapsilosis (n = 118), and C. tropicalis (n = 103) from rectal swabs (n = 754 patients) were studied. Genotyping was conducted using species-specific microsatellite markers. Rectal swab genotypes were compared with previously studied blood (n = 814) and intra-abdominal (n = 202) genotypes. Results: A total of 36/754 patients had the same Candida spp. isolated from blood cultures, intra-abdominal samples, or both; these patients had candidemia (n = 18), intra-abdominal candidiasis (n = 11), both clinical forms (n = 1), and non-significant isolation (n = 6). Genotypes matching the rectal swab and their blood cultures (84.2%) or their intra-abdominal samples (92.3%) were found in most of the significant patients. We detected 656 genotypes from rectal swabs, 88.4% of which were singletons and 11.6% were clusters. Of these 656 rectal swab genotypes, 94 (14.3%) were also detected in blood cultures and 34 (5.2%) in intra-abdominal samples. Of the rectal swab clusters, 62.7% were previously defined as a widespread genotype. Conclusions: Our study pinpoints the gastrointestinal tract as a potential reservoir of potentially invasive Candida spp. genotypes. MDPI 2023-07-06 /pmc/articles/PMC10381600/ /pubmed/37504721 http://dx.doi.org/10.3390/jof9070732 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mesquida, Aina
Machado, Marina
Dávila-Cherres, Lorena
Vicente, Teresa
Sánchez-Carrillo, Carlos
Alcalá, Luis
Reigadas, Elena
Muñoz, Patricia
Guinea, Jesús
Escribano, Pilar
The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title_full The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title_fullStr The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title_full_unstemmed The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title_short The Gastrointestinal Tract Is Pinpointed as a Reservoir of Candida albicans, Candida parapsilosis, and Candida tropicalis Genotypes Found in Blood and Intra-Abdominal Samples
title_sort gastrointestinal tract is pinpointed as a reservoir of candida albicans, candida parapsilosis, and candida tropicalis genotypes found in blood and intra-abdominal samples
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381600/
https://www.ncbi.nlm.nih.gov/pubmed/37504721
http://dx.doi.org/10.3390/jof9070732
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