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Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective

Neck dissection for cervical lymph node metastasis is an established procedure for head and neck cancer (HNC). However, with the advent of immunotherapy, head and neck surgical oncologists need to rethink removing all lymph nodes, including those with immune function. We investigated the anti-cancer...

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Autores principales: Nakamura, Hiromu, Ogawa, Tetsuya, Yamanaka, Shunpei, Inukai, Daisuke, Maruo, Takashi, Takahara, Taishi, Satou, Akira, Tsuzuki, Toyonori, Suzuki, Susumu, Ueda, Ryuzo, Fujimoto, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381643/
https://www.ncbi.nlm.nih.gov/pubmed/37511787
http://dx.doi.org/10.3390/jpm13071174
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author Nakamura, Hiromu
Ogawa, Tetsuya
Yamanaka, Shunpei
Inukai, Daisuke
Maruo, Takashi
Takahara, Taishi
Satou, Akira
Tsuzuki, Toyonori
Suzuki, Susumu
Ueda, Ryuzo
Fujimoto, Yasushi
author_facet Nakamura, Hiromu
Ogawa, Tetsuya
Yamanaka, Shunpei
Inukai, Daisuke
Maruo, Takashi
Takahara, Taishi
Satou, Akira
Tsuzuki, Toyonori
Suzuki, Susumu
Ueda, Ryuzo
Fujimoto, Yasushi
author_sort Nakamura, Hiromu
collection PubMed
description Neck dissection for cervical lymph node metastasis is an established procedure for head and neck cancer (HNC). However, with the advent of immunotherapy, head and neck surgical oncologists need to rethink removing all lymph nodes, including those with immune function. We investigated the anti-cancer immune response of the cervical lymph nodes in four patients with human papillomavirus type 16 (HPV16)-positive head and neck squamous cell carcinoma. Using lymphocytes extracted from local, metastatic, and non-metastatic lymph nodes and peripheral blood from these patients, we performed an intracellular flow cytometric cytokine assay using anti-IFNγ and anti-TNF-α monoclonal antibodies to detect HPV16 E6- and E7-specific T cells. HPV status and p16 immunostaining were determined by in situ detection using the HPV RNAscope method and immunohistochemistry. In one case, E6-specific and E7-specific CD8+ T cells were detected in proximal metastatic nodes and distal non-metastatic nodes. This finding suggests that non-metastatic nodes should be preserved for their immune function during neck dissection and that the immune function of non-metastatic lymph nodes is important when administering immunotherapy. In this context, head and neck surgical oncologists treating HNC should consider the place of immunotherapy and neck dissection in the treatment of HNC.
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spelling pubmed-103816432023-07-29 Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective Nakamura, Hiromu Ogawa, Tetsuya Yamanaka, Shunpei Inukai, Daisuke Maruo, Takashi Takahara, Taishi Satou, Akira Tsuzuki, Toyonori Suzuki, Susumu Ueda, Ryuzo Fujimoto, Yasushi J Pers Med Article Neck dissection for cervical lymph node metastasis is an established procedure for head and neck cancer (HNC). However, with the advent of immunotherapy, head and neck surgical oncologists need to rethink removing all lymph nodes, including those with immune function. We investigated the anti-cancer immune response of the cervical lymph nodes in four patients with human papillomavirus type 16 (HPV16)-positive head and neck squamous cell carcinoma. Using lymphocytes extracted from local, metastatic, and non-metastatic lymph nodes and peripheral blood from these patients, we performed an intracellular flow cytometric cytokine assay using anti-IFNγ and anti-TNF-α monoclonal antibodies to detect HPV16 E6- and E7-specific T cells. HPV status and p16 immunostaining were determined by in situ detection using the HPV RNAscope method and immunohistochemistry. In one case, E6-specific and E7-specific CD8+ T cells were detected in proximal metastatic nodes and distal non-metastatic nodes. This finding suggests that non-metastatic nodes should be preserved for their immune function during neck dissection and that the immune function of non-metastatic lymph nodes is important when administering immunotherapy. In this context, head and neck surgical oncologists treating HNC should consider the place of immunotherapy and neck dissection in the treatment of HNC. MDPI 2023-07-22 /pmc/articles/PMC10381643/ /pubmed/37511787 http://dx.doi.org/10.3390/jpm13071174 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nakamura, Hiromu
Ogawa, Tetsuya
Yamanaka, Shunpei
Inukai, Daisuke
Maruo, Takashi
Takahara, Taishi
Satou, Akira
Tsuzuki, Toyonori
Suzuki, Susumu
Ueda, Ryuzo
Fujimoto, Yasushi
Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title_full Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title_fullStr Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title_full_unstemmed Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title_short Immune Status of Cervical Lymph Nodes in Head and Neck Cancer—A Surgical Oncology Perspective
title_sort immune status of cervical lymph nodes in head and neck cancer—a surgical oncology perspective
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381643/
https://www.ncbi.nlm.nih.gov/pubmed/37511787
http://dx.doi.org/10.3390/jpm13071174
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