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Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I

The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene cytochrome oxidase subunit I (COI). The sequences of tick samples from camels in three regions of Ha’il we...

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Autores principales: Bardakci, Fevzi, Al-Subaie, Sarah Hilan Mohammed, Badraoui, Riadh, Adnan, Mohd, Siddiqui, Arif Jamal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381736/
https://www.ncbi.nlm.nih.gov/pubmed/37511909
http://dx.doi.org/10.3390/life13071535
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author Bardakci, Fevzi
Al-Subaie, Sarah Hilan Mohammed
Badraoui, Riadh
Adnan, Mohd
Siddiqui, Arif Jamal
author_facet Bardakci, Fevzi
Al-Subaie, Sarah Hilan Mohammed
Badraoui, Riadh
Adnan, Mohd
Siddiqui, Arif Jamal
author_sort Bardakci, Fevzi
collection PubMed
description The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene cytochrome oxidase subunit I (COI). The sequences of tick samples from camels in three regions of Ha’il were aligned with those previously reported from different geographic regions, revealing nine haplotypes, of which six were newly described in this study for the first time. These haplotypes were used to determine their phylogenetic relationships using the maximum likelihood method, displaying two distinct clades corresponding to Hyalomma dromedarii and H. impeltatum. Moreover, the haplotypes showing the highest homology with those deposited in NCBI-GenBank from different geographic regions, including Saudi Arabia, were obtained and combined to determine their phylogenetic relationships among them. The results showed that the haplotypes belonging to two clades were grouped with those previously determined as H. dromedarii and H. impeltatum. Moreover, the presence of H. scupense (syn. H. detritum) together with H. impeltatum suggests possible asymmetrical hybridization and mitochondrial introgression between these species. H. scupense infesting different mammal species apart from camels were also clustered in a different clade, indicating the presence of different lineages of this species that show different host specificities.
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spelling pubmed-103817362023-07-29 Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I Bardakci, Fevzi Al-Subaie, Sarah Hilan Mohammed Badraoui, Riadh Adnan, Mohd Siddiqui, Arif Jamal Life (Basel) Article The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene cytochrome oxidase subunit I (COI). The sequences of tick samples from camels in three regions of Ha’il were aligned with those previously reported from different geographic regions, revealing nine haplotypes, of which six were newly described in this study for the first time. These haplotypes were used to determine their phylogenetic relationships using the maximum likelihood method, displaying two distinct clades corresponding to Hyalomma dromedarii and H. impeltatum. Moreover, the haplotypes showing the highest homology with those deposited in NCBI-GenBank from different geographic regions, including Saudi Arabia, were obtained and combined to determine their phylogenetic relationships among them. The results showed that the haplotypes belonging to two clades were grouped with those previously determined as H. dromedarii and H. impeltatum. Moreover, the presence of H. scupense (syn. H. detritum) together with H. impeltatum suggests possible asymmetrical hybridization and mitochondrial introgression between these species. H. scupense infesting different mammal species apart from camels were also clustered in a different clade, indicating the presence of different lineages of this species that show different host specificities. MDPI 2023-07-10 /pmc/articles/PMC10381736/ /pubmed/37511909 http://dx.doi.org/10.3390/life13071535 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bardakci, Fevzi
Al-Subaie, Sarah Hilan Mohammed
Badraoui, Riadh
Adnan, Mohd
Siddiqui, Arif Jamal
Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title_full Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title_fullStr Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title_full_unstemmed Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title_short Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial Cytochrome oxidase subunit I
title_sort molecular characterization of hard ticks infesting camels in the northern region of saudi arabia using the barcoding gene, mitochondrial cytochrome oxidase subunit i
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381736/
https://www.ncbi.nlm.nih.gov/pubmed/37511909
http://dx.doi.org/10.3390/life13071535
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