Cargando…

Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation

Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated w...

Descripción completa

Detalles Bibliográficos
Autores principales: Papakonstantinou, Georgios I., Psalla, Dimitra, Pourlis, Aris, Stylianaki, Ioanna, Athanasiou, Labrini V., Tzika, Eleni, Meletis, Eleftherios, Kostoulas, Polychronis, Maragkakis, George, Christodoulopoulos, Georgios, Papaioannou, Nikolaos, Papatsiros, Vasileios G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381811/
https://www.ncbi.nlm.nih.gov/pubmed/37511984
http://dx.doi.org/10.3390/life13071609
_version_ 1785080536221351936
author Papakonstantinou, Georgios I.
Psalla, Dimitra
Pourlis, Aris
Stylianaki, Ioanna
Athanasiou, Labrini V.
Tzika, Eleni
Meletis, Eleftherios
Kostoulas, Polychronis
Maragkakis, George
Christodoulopoulos, Georgios
Papaioannou, Nikolaos
Papatsiros, Vasileios G.
author_facet Papakonstantinou, Georgios I.
Psalla, Dimitra
Pourlis, Aris
Stylianaki, Ioanna
Athanasiou, Labrini V.
Tzika, Eleni
Meletis, Eleftherios
Kostoulas, Polychronis
Maragkakis, George
Christodoulopoulos, Georgios
Papaioannou, Nikolaos
Papatsiros, Vasileios G.
author_sort Papakonstantinou, Georgios I.
collection PubMed
description Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated with PRRSV-1 MLV. In total, 25 gilts were vaccinated at late gestation (100th day) and were divided into five groups according to the different vaccines (Vac) used: no vaccine—control group, Vac-1—strain DV, Vac-2—strain VP-046 BIS, Vac-3—strain 94881, Vac-4—strain 96V198. Within the first 0–9 h of the farrowing, blood samples were collected from all newborn piglets and lung samples were exanimated grossly, histopathologically and with scanning electron microscopy. PRRSV (RT-PCR-positive) and antibodies were detected in the serum of piglets from gilts vaccinated with Vac-2. In these piglets, moderate to severe interstitial pneumonia with thickened alveolar septa was noticed. Type II pneumocyte hyperplasia was also observed. The rest of the trial piglets showed unremarkable lung lesions. Phylogenetic analysis revealed the 98.7% similarity of the PRRSV field strain (GR 2019-1) to the PRRS MLV vaccine strain VP-046 BIS. In conclusion, the Vac-2 PRRSV vaccine strain can act as an infectious strain when vaccination is administrated at late gestation, causing lung lesions.
format Online
Article
Text
id pubmed-10381811
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103818112023-07-29 Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation Papakonstantinou, Georgios I. Psalla, Dimitra Pourlis, Aris Stylianaki, Ioanna Athanasiou, Labrini V. Tzika, Eleni Meletis, Eleftherios Kostoulas, Polychronis Maragkakis, George Christodoulopoulos, Georgios Papaioannou, Nikolaos Papatsiros, Vasileios G. Life (Basel) Article Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated with PRRSV-1 MLV. In total, 25 gilts were vaccinated at late gestation (100th day) and were divided into five groups according to the different vaccines (Vac) used: no vaccine—control group, Vac-1—strain DV, Vac-2—strain VP-046 BIS, Vac-3—strain 94881, Vac-4—strain 96V198. Within the first 0–9 h of the farrowing, blood samples were collected from all newborn piglets and lung samples were exanimated grossly, histopathologically and with scanning electron microscopy. PRRSV (RT-PCR-positive) and antibodies were detected in the serum of piglets from gilts vaccinated with Vac-2. In these piglets, moderate to severe interstitial pneumonia with thickened alveolar septa was noticed. Type II pneumocyte hyperplasia was also observed. The rest of the trial piglets showed unremarkable lung lesions. Phylogenetic analysis revealed the 98.7% similarity of the PRRSV field strain (GR 2019-1) to the PRRS MLV vaccine strain VP-046 BIS. In conclusion, the Vac-2 PRRSV vaccine strain can act as an infectious strain when vaccination is administrated at late gestation, causing lung lesions. MDPI 2023-07-23 /pmc/articles/PMC10381811/ /pubmed/37511984 http://dx.doi.org/10.3390/life13071609 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papakonstantinou, Georgios I.
Psalla, Dimitra
Pourlis, Aris
Stylianaki, Ioanna
Athanasiou, Labrini V.
Tzika, Eleni
Meletis, Eleftherios
Kostoulas, Polychronis
Maragkakis, George
Christodoulopoulos, Georgios
Papaioannou, Nikolaos
Papatsiros, Vasileios G.
Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title_full Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title_fullStr Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title_full_unstemmed Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title_short Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
title_sort histopathological pulmonary lesions in 1st-day newborn piglets derived from prrsv-1 mlv vaccinated sows at the last stage of gestation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381811/
https://www.ncbi.nlm.nih.gov/pubmed/37511984
http://dx.doi.org/10.3390/life13071609
work_keys_str_mv AT papakonstantinougeorgiosi histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT psalladimitra histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT pourlisaris histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT stylianakiioanna histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT athanasioulabriniv histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT tzikaeleni histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT meletiseleftherios histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT kostoulaspolychronis histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT maragkakisgeorge histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT christodoulopoulosgeorgios histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT papaioannounikolaos histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation
AT papatsirosvasileiosg histopathologicalpulmonarylesionsin1stdaynewbornpigletsderivedfromprrsv1mlvvaccinatedsowsatthelaststageofgestation