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Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation
Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381811/ https://www.ncbi.nlm.nih.gov/pubmed/37511984 http://dx.doi.org/10.3390/life13071609 |
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author | Papakonstantinou, Georgios I. Psalla, Dimitra Pourlis, Aris Stylianaki, Ioanna Athanasiou, Labrini V. Tzika, Eleni Meletis, Eleftherios Kostoulas, Polychronis Maragkakis, George Christodoulopoulos, Georgios Papaioannou, Nikolaos Papatsiros, Vasileios G. |
author_facet | Papakonstantinou, Georgios I. Psalla, Dimitra Pourlis, Aris Stylianaki, Ioanna Athanasiou, Labrini V. Tzika, Eleni Meletis, Eleftherios Kostoulas, Polychronis Maragkakis, George Christodoulopoulos, Georgios Papaioannou, Nikolaos Papatsiros, Vasileios G. |
author_sort | Papakonstantinou, Georgios I. |
collection | PubMed |
description | Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated with PRRSV-1 MLV. In total, 25 gilts were vaccinated at late gestation (100th day) and were divided into five groups according to the different vaccines (Vac) used: no vaccine—control group, Vac-1—strain DV, Vac-2—strain VP-046 BIS, Vac-3—strain 94881, Vac-4—strain 96V198. Within the first 0–9 h of the farrowing, blood samples were collected from all newborn piglets and lung samples were exanimated grossly, histopathologically and with scanning electron microscopy. PRRSV (RT-PCR-positive) and antibodies were detected in the serum of piglets from gilts vaccinated with Vac-2. In these piglets, moderate to severe interstitial pneumonia with thickened alveolar septa was noticed. Type II pneumocyte hyperplasia was also observed. The rest of the trial piglets showed unremarkable lung lesions. Phylogenetic analysis revealed the 98.7% similarity of the PRRSV field strain (GR 2019-1) to the PRRS MLV vaccine strain VP-046 BIS. In conclusion, the Vac-2 PRRSV vaccine strain can act as an infectious strain when vaccination is administrated at late gestation, causing lung lesions. |
format | Online Article Text |
id | pubmed-10381811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103818112023-07-29 Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation Papakonstantinou, Georgios I. Psalla, Dimitra Pourlis, Aris Stylianaki, Ioanna Athanasiou, Labrini V. Tzika, Eleni Meletis, Eleftherios Kostoulas, Polychronis Maragkakis, George Christodoulopoulos, Georgios Papaioannou, Nikolaos Papatsiros, Vasileios G. Life (Basel) Article Modified live virus (MLV) vaccines for the control of porcine respiratory and reproductive syndrome virus (PRRSV) have been associated with the vertical and horizontal transmission of vaccine viruses. The present study aimed to describe pathological lung lesions in piglets born by gilts vaccinated with PRRSV-1 MLV. In total, 25 gilts were vaccinated at late gestation (100th day) and were divided into five groups according to the different vaccines (Vac) used: no vaccine—control group, Vac-1—strain DV, Vac-2—strain VP-046 BIS, Vac-3—strain 94881, Vac-4—strain 96V198. Within the first 0–9 h of the farrowing, blood samples were collected from all newborn piglets and lung samples were exanimated grossly, histopathologically and with scanning electron microscopy. PRRSV (RT-PCR-positive) and antibodies were detected in the serum of piglets from gilts vaccinated with Vac-2. In these piglets, moderate to severe interstitial pneumonia with thickened alveolar septa was noticed. Type II pneumocyte hyperplasia was also observed. The rest of the trial piglets showed unremarkable lung lesions. Phylogenetic analysis revealed the 98.7% similarity of the PRRSV field strain (GR 2019-1) to the PRRS MLV vaccine strain VP-046 BIS. In conclusion, the Vac-2 PRRSV vaccine strain can act as an infectious strain when vaccination is administrated at late gestation, causing lung lesions. MDPI 2023-07-23 /pmc/articles/PMC10381811/ /pubmed/37511984 http://dx.doi.org/10.3390/life13071609 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Papakonstantinou, Georgios I. Psalla, Dimitra Pourlis, Aris Stylianaki, Ioanna Athanasiou, Labrini V. Tzika, Eleni Meletis, Eleftherios Kostoulas, Polychronis Maragkakis, George Christodoulopoulos, Georgios Papaioannou, Nikolaos Papatsiros, Vasileios G. Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title | Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title_full | Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title_fullStr | Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title_full_unstemmed | Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title_short | Histopathological Pulmonary Lesions in 1st-Day Newborn Piglets Derived from PRRSV-1 MLV Vaccinated Sows at the Last Stage of Gestation |
title_sort | histopathological pulmonary lesions in 1st-day newborn piglets derived from prrsv-1 mlv vaccinated sows at the last stage of gestation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381811/ https://www.ncbi.nlm.nih.gov/pubmed/37511984 http://dx.doi.org/10.3390/life13071609 |
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