Cargando…
A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series
Background: Senescence, a characteristic of cellular aging and inflammation, has been linked to the acceleration of osteoarthritis. The purpose of this study is to prospectively identify, measure, and compare senescent profiles in synovial fluid and peripheral blood in patients with an acute knee in...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381817/ https://www.ncbi.nlm.nih.gov/pubmed/37511942 http://dx.doi.org/10.3390/life13071567 |
_version_ | 1785080537731301376 |
---|---|
author | Waltz, Robert A. Whitney, Kaitlyn E. Duke, Victoria R. Kloser, Heidi Huard, Charles Provencher, Matthew T. Philippon, Marc J. Bahney, Chelsea Godin, Jonathan A. Huard, Johnny |
author_facet | Waltz, Robert A. Whitney, Kaitlyn E. Duke, Victoria R. Kloser, Heidi Huard, Charles Provencher, Matthew T. Philippon, Marc J. Bahney, Chelsea Godin, Jonathan A. Huard, Johnny |
author_sort | Waltz, Robert A. |
collection | PubMed |
description | Background: Senescence, a characteristic of cellular aging and inflammation, has been linked to the acceleration of osteoarthritis. The purpose of this study is to prospectively identify, measure, and compare senescent profiles in synovial fluid and peripheral blood in patients with an acute knee injury within 48 h. Methods: Seven subjects, aged 18–60 years, with an acute ACL tear with effusion were prospectively enrolled. Synovial fluid and peripheral blood samples were collected and analyzed by flow cytometry, using senescent markers C12FDG and CD87. The senescent versus pro-regenerative phenotype was probed at a gene and protein level using qRT-PCR and multiplex immunoassays. Results: C(12)FDG and CD87 positive senescent cells were detected in the synovial fluid and peripheral blood of all patients. Pro-inflammatory IL-1β gene expression measured in synovial fluid was significantly higher (p = 0.0156) than systemic/blood expression. Senescent-associated factor MMP-3 and regenerative factor TIMP-2 were significantly higher in synovial fluid compared to blood serum. Senescent-associated factor MMP-9 and regenerative factor TGFβ-2 were significantly elevated in serum compared to synovial fluid. Correlation analysis revealed that C12FDG(++)/CD87(++) senescent cells in synovial fluid positively correlated with age-related growth-regulated-oncogene (ρ = 1.00, p < 0.001), IFNγ (ρ = 1.00, p < 0.001), IL-8 (ρ = 0.90, p = 0.0374), and gene marker p16 (ρ = 0.83, p = 0.0416). Conclusions: There is an abundance of senescent cells locally and systemically after an acute ACL tear without a significant difference between those present in peripheral blood compared to synovial fluid. This preliminary data may have a role in identifying strategies to modify the acute environment within the synovial fluid, either at the time of acute ligament injury or reconstruction surgery. |
format | Online Article Text |
id | pubmed-10381817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103818172023-07-29 A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series Waltz, Robert A. Whitney, Kaitlyn E. Duke, Victoria R. Kloser, Heidi Huard, Charles Provencher, Matthew T. Philippon, Marc J. Bahney, Chelsea Godin, Jonathan A. Huard, Johnny Life (Basel) Article Background: Senescence, a characteristic of cellular aging and inflammation, has been linked to the acceleration of osteoarthritis. The purpose of this study is to prospectively identify, measure, and compare senescent profiles in synovial fluid and peripheral blood in patients with an acute knee injury within 48 h. Methods: Seven subjects, aged 18–60 years, with an acute ACL tear with effusion were prospectively enrolled. Synovial fluid and peripheral blood samples were collected and analyzed by flow cytometry, using senescent markers C12FDG and CD87. The senescent versus pro-regenerative phenotype was probed at a gene and protein level using qRT-PCR and multiplex immunoassays. Results: C(12)FDG and CD87 positive senescent cells were detected in the synovial fluid and peripheral blood of all patients. Pro-inflammatory IL-1β gene expression measured in synovial fluid was significantly higher (p = 0.0156) than systemic/blood expression. Senescent-associated factor MMP-3 and regenerative factor TIMP-2 were significantly higher in synovial fluid compared to blood serum. Senescent-associated factor MMP-9 and regenerative factor TGFβ-2 were significantly elevated in serum compared to synovial fluid. Correlation analysis revealed that C12FDG(++)/CD87(++) senescent cells in synovial fluid positively correlated with age-related growth-regulated-oncogene (ρ = 1.00, p < 0.001), IFNγ (ρ = 1.00, p < 0.001), IL-8 (ρ = 0.90, p = 0.0374), and gene marker p16 (ρ = 0.83, p = 0.0416). Conclusions: There is an abundance of senescent cells locally and systemically after an acute ACL tear without a significant difference between those present in peripheral blood compared to synovial fluid. This preliminary data may have a role in identifying strategies to modify the acute environment within the synovial fluid, either at the time of acute ligament injury or reconstruction surgery. MDPI 2023-07-15 /pmc/articles/PMC10381817/ /pubmed/37511942 http://dx.doi.org/10.3390/life13071567 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Waltz, Robert A. Whitney, Kaitlyn E. Duke, Victoria R. Kloser, Heidi Huard, Charles Provencher, Matthew T. Philippon, Marc J. Bahney, Chelsea Godin, Jonathan A. Huard, Johnny A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title | A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title_full | A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title_fullStr | A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title_full_unstemmed | A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title_short | A Systemic and Local Comparison of Senescence in an Acute Anterior Cruciate Ligament Injury—A Pilot Case Series |
title_sort | systemic and local comparison of senescence in an acute anterior cruciate ligament injury—a pilot case series |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381817/ https://www.ncbi.nlm.nih.gov/pubmed/37511942 http://dx.doi.org/10.3390/life13071567 |
work_keys_str_mv | AT waltzroberta asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT whitneykaitlyne asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT dukevictoriar asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT kloserheidi asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT huardcharles asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT provenchermatthewt asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT philipponmarcj asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT bahneychelsea asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT godinjonathana asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT huardjohnny asystemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT waltzroberta systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT whitneykaitlyne systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT dukevictoriar systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT kloserheidi systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT huardcharles systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT provenchermatthewt systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT philipponmarcj systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT bahneychelsea systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT godinjonathana systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries AT huardjohnny systemicandlocalcomparisonofsenescenceinanacuteanteriorcruciateligamentinjuryapilotcaseseries |