Cargando…

Case Studies in Molecular Network-Guided Marine Biodiscovery

In reviewing a selection of recent case studies from our laboratory, we revealed some lessons learned and benefits accrued from the application of mass spectrometry (MS/MS) molecular networking in the field of marine sponge natural products. Molecular networking proved pivotal to our discovery of ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Khushi, Shamsunnahar, Salim, Angela A., Capon, Robert J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381900/
https://www.ncbi.nlm.nih.gov/pubmed/37504944
http://dx.doi.org/10.3390/md21070413
_version_ 1785080558734278656
author Khushi, Shamsunnahar
Salim, Angela A.
Capon, Robert J.
author_facet Khushi, Shamsunnahar
Salim, Angela A.
Capon, Robert J.
author_sort Khushi, Shamsunnahar
collection PubMed
description In reviewing a selection of recent case studies from our laboratory, we revealed some lessons learned and benefits accrued from the application of mass spectrometry (MS/MS) molecular networking in the field of marine sponge natural products. Molecular networking proved pivotal to our discovery of many new natural products and even new classes of natural product, some of which were opaque to alternate dereplication and prioritization strategies. Case studies included the discovery of: (i) trachycladindoles, an exceptionally rare class of bioactive indole alkaloid previously only known from a single southern Australia sample of Trachycladus laevispirulifer; (ii) dysidealactams, an unprecedented class of sesquiterpene glycinyl-lactam and glycinyl-imide from a Dysidea sp., a sponge genera often discounted as having been exhaustively studied; (iii) cacolides, an unprecedented family of sesterterpene α-methyl-γ-hydroxybutenolides from a Cacospongia sp., all too easily mischaracterized and deprioritized during dereplication as a well-known class of sponge sesterterpene tetronic acids; and (iv) thorectandrins, a new class of indole alkaloid which revealed unexpected insights into the chemical and biological properties of the aplysinopsins, one of the earliest and more extensively reported class of sponge natural products.
format Online
Article
Text
id pubmed-10381900
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103819002023-07-29 Case Studies in Molecular Network-Guided Marine Biodiscovery Khushi, Shamsunnahar Salim, Angela A. Capon, Robert J. Mar Drugs Perspective In reviewing a selection of recent case studies from our laboratory, we revealed some lessons learned and benefits accrued from the application of mass spectrometry (MS/MS) molecular networking in the field of marine sponge natural products. Molecular networking proved pivotal to our discovery of many new natural products and even new classes of natural product, some of which were opaque to alternate dereplication and prioritization strategies. Case studies included the discovery of: (i) trachycladindoles, an exceptionally rare class of bioactive indole alkaloid previously only known from a single southern Australia sample of Trachycladus laevispirulifer; (ii) dysidealactams, an unprecedented class of sesquiterpene glycinyl-lactam and glycinyl-imide from a Dysidea sp., a sponge genera often discounted as having been exhaustively studied; (iii) cacolides, an unprecedented family of sesterterpene α-methyl-γ-hydroxybutenolides from a Cacospongia sp., all too easily mischaracterized and deprioritized during dereplication as a well-known class of sponge sesterterpene tetronic acids; and (iv) thorectandrins, a new class of indole alkaloid which revealed unexpected insights into the chemical and biological properties of the aplysinopsins, one of the earliest and more extensively reported class of sponge natural products. MDPI 2023-07-20 /pmc/articles/PMC10381900/ /pubmed/37504944 http://dx.doi.org/10.3390/md21070413 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Khushi, Shamsunnahar
Salim, Angela A.
Capon, Robert J.
Case Studies in Molecular Network-Guided Marine Biodiscovery
title Case Studies in Molecular Network-Guided Marine Biodiscovery
title_full Case Studies in Molecular Network-Guided Marine Biodiscovery
title_fullStr Case Studies in Molecular Network-Guided Marine Biodiscovery
title_full_unstemmed Case Studies in Molecular Network-Guided Marine Biodiscovery
title_short Case Studies in Molecular Network-Guided Marine Biodiscovery
title_sort case studies in molecular network-guided marine biodiscovery
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381900/
https://www.ncbi.nlm.nih.gov/pubmed/37504944
http://dx.doi.org/10.3390/md21070413
work_keys_str_mv AT khushishamsunnahar casestudiesinmolecularnetworkguidedmarinebiodiscovery
AT salimangelaa casestudiesinmolecularnetworkguidedmarinebiodiscovery
AT caponrobertj casestudiesinmolecularnetworkguidedmarinebiodiscovery