ASSURED-optimized CRISPR protocol for knockout/SNP knockin in hiPSCs

CRISPR-Cas9 technology coupled with human induced pluripotent stem cells allows precise disease modeling in pluripotent cells and subsequently derived specialized cell types. Here, we present an optimized CRISPR-Cas9 pipeline, ASSURED (affordable, successful, specific, user-friendly, rapid, efficien...

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Detalles Bibliográficos
Autores principales: Ludwik, Katarzyna A., Telugu, Narasimha, Schommer, Sandra, Stachelscheid, Harald, Diecke, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382939/
https://www.ncbi.nlm.nih.gov/pubmed/37481731
http://dx.doi.org/10.1016/j.xpro.2023.102406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382939/
https://www.ncbi.nlm.nih.gov/pubmed/37481731
http://dx.doi.org/10.1016/j.xpro.2023.102406

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