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Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses
The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100–10,000 cells with at least 80%–90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe steps fo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382986/ https://www.ncbi.nlm.nih.gov/pubmed/37480562 http://dx.doi.org/10.1016/j.xpro.2023.102464 |
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author | Chen, Hao Peng, Junya Liu, Lulu Huang, Dan Zhao, Yupei Wu, Wenming |
author_facet | Chen, Hao Peng, Junya Liu, Lulu Huang, Dan Zhao, Yupei Wu, Wenming |
author_sort | Chen, Hao |
collection | PubMed |
description | The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100–10,000 cells with at least 80%–90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe steps for sample harvesting, procurement, tissue digestion, and cell purification. Subsequently, isolated cells can be further applied to single-cell profiling, for example, single-cell RNA sequencing and single-cell ATAC-seq using 10× Genomics platform. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019)(1) and Li et al. (2021).(2) |
format | Online Article Text |
id | pubmed-10382986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103829862023-07-30 Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses Chen, Hao Peng, Junya Liu, Lulu Huang, Dan Zhao, Yupei Wu, Wenming STAR Protoc Protocol The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100–10,000 cells with at least 80%–90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe steps for sample harvesting, procurement, tissue digestion, and cell purification. Subsequently, isolated cells can be further applied to single-cell profiling, for example, single-cell RNA sequencing and single-cell ATAC-seq using 10× Genomics platform. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019)(1) and Li et al. (2021).(2) Elsevier 2023-07-24 /pmc/articles/PMC10382986/ /pubmed/37480562 http://dx.doi.org/10.1016/j.xpro.2023.102464 Text en © 2023 The Authors. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Chen, Hao Peng, Junya Liu, Lulu Huang, Dan Zhao, Yupei Wu, Wenming Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title | Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title_full | Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title_fullStr | Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title_full_unstemmed | Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title_short | Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
title_sort | protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382986/ https://www.ncbi.nlm.nih.gov/pubmed/37480562 http://dx.doi.org/10.1016/j.xpro.2023.102464 |
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