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5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer
Novel fibrous materials with diverse biological properties containing a model drug of the 8-hydroxyquinoline group—5-amino-8-hydroxyquinoline (5A8Q)—were fabricated using a one-pot method by electrospinning poly(vinyl alcohol) (PVA)/carboxymethyl cellulose (CMC)/5A8Q solutions. Experiments were perf...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383330/ https://www.ncbi.nlm.nih.gov/pubmed/37514529 http://dx.doi.org/10.3390/polym15143140 |
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author | Ignatova, Milena Manolova, Nevena Rashkov, Iliya Georgieva, Ani Toshkova, Reneta Markova, Nadya |
author_facet | Ignatova, Milena Manolova, Nevena Rashkov, Iliya Georgieva, Ani Toshkova, Reneta Markova, Nadya |
author_sort | Ignatova, Milena |
collection | PubMed |
description | Novel fibrous materials with diverse biological properties containing a model drug of the 8-hydroxyquinoline group—5-amino-8-hydroxyquinoline (5A8Q)—were fabricated using a one-pot method by electrospinning poly(vinyl alcohol) (PVA)/carboxymethyl cellulose (CMC)/5A8Q solutions. Experiments were performed to prepare Cu(2+) (Fe(3+)) complexes of the crosslinked PVA/CMC/5A8Q materials. The formation of complexes was proven by using scanning electron microscopy (SEM), attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy, energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). The release of 5A8Q and 5A8Q.Cu(2+) (Fe(3+)) was studied and their in vitro release profiles were mostly impacted by the hydrophilic/hydrophobic properties of the materials. The performed microbiological assays revealed that fibrous materials containing 5A8Q and their complexes exhibited good antibacterial and antifungal efficacy. Their activity was stronger against bacteria S. aureus than against bacteria E. coli and fungi C. albicans. Cell viability tests using MTT showed that the presence of 5A8Q and its complexes in the fibrous materials resulted in a significant decrease in the HeLa and MCF-7 cancer cell viability for the various times of cell incubation. Moreover, the observed cytotoxicity of the mats against cancer cells was greater than that against non-cancer HaCaT keratinocytes. All these properties make the novel materials potential candidates for the design of wound healing materials and as drug delivery systems for local therapy of cervical and breast cancer. |
format | Online Article Text |
id | pubmed-10383330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103833302023-07-30 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer Ignatova, Milena Manolova, Nevena Rashkov, Iliya Georgieva, Ani Toshkova, Reneta Markova, Nadya Polymers (Basel) Article Novel fibrous materials with diverse biological properties containing a model drug of the 8-hydroxyquinoline group—5-amino-8-hydroxyquinoline (5A8Q)—were fabricated using a one-pot method by electrospinning poly(vinyl alcohol) (PVA)/carboxymethyl cellulose (CMC)/5A8Q solutions. Experiments were performed to prepare Cu(2+) (Fe(3+)) complexes of the crosslinked PVA/CMC/5A8Q materials. The formation of complexes was proven by using scanning electron microscopy (SEM), attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy, energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). The release of 5A8Q and 5A8Q.Cu(2+) (Fe(3+)) was studied and their in vitro release profiles were mostly impacted by the hydrophilic/hydrophobic properties of the materials. The performed microbiological assays revealed that fibrous materials containing 5A8Q and their complexes exhibited good antibacterial and antifungal efficacy. Their activity was stronger against bacteria S. aureus than against bacteria E. coli and fungi C. albicans. Cell viability tests using MTT showed that the presence of 5A8Q and its complexes in the fibrous materials resulted in a significant decrease in the HeLa and MCF-7 cancer cell viability for the various times of cell incubation. Moreover, the observed cytotoxicity of the mats against cancer cells was greater than that against non-cancer HaCaT keratinocytes. All these properties make the novel materials potential candidates for the design of wound healing materials and as drug delivery systems for local therapy of cervical and breast cancer. MDPI 2023-07-24 /pmc/articles/PMC10383330/ /pubmed/37514529 http://dx.doi.org/10.3390/polym15143140 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ignatova, Milena Manolova, Nevena Rashkov, Iliya Georgieva, Ani Toshkova, Reneta Markova, Nadya 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title | 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title_full | 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title_fullStr | 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title_full_unstemmed | 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title_short | 5-Amino-8-hydroxyquinoline-containing Electrospun Materials Based on Poly(vinyl alcohol) and Carboxymethyl Cellulose and Their Cu(2+) and Fe(3+) Complexes with Diverse Biological Properties: Antibacterial, Antifungal and Anticancer |
title_sort | 5-amino-8-hydroxyquinoline-containing electrospun materials based on poly(vinyl alcohol) and carboxymethyl cellulose and their cu(2+) and fe(3+) complexes with diverse biological properties: antibacterial, antifungal and anticancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383330/ https://www.ncbi.nlm.nih.gov/pubmed/37514529 http://dx.doi.org/10.3390/polym15143140 |
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