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Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum

Bacterial wilt disease of tomato (Solanum lycopersicum L.), incited by Ralstonia solanacearum (Smith), is a serious agricultural problem in India. In this investigation, chemical mutagenic agents (NTG and HNO(2) treatment) and ultraviolet (UV) irradiation have been used to enhance the antagonistic p...

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Autores principales: Yadav, Dhananjay Kumar, Devappa, Venkatappa, Kashyap, Abhijeet Shankar, Kumar, Narendra, Rana, V. S., Sunita, Kumari, Singh, Dinesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383371/
https://www.ncbi.nlm.nih.gov/pubmed/37512962
http://dx.doi.org/10.3390/microorganisms11071790
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author Yadav, Dhananjay Kumar
Devappa, Venkatappa
Kashyap, Abhijeet Shankar
Kumar, Narendra
Rana, V. S.
Sunita, Kumari
Singh, Dinesh
author_facet Yadav, Dhananjay Kumar
Devappa, Venkatappa
Kashyap, Abhijeet Shankar
Kumar, Narendra
Rana, V. S.
Sunita, Kumari
Singh, Dinesh
author_sort Yadav, Dhananjay Kumar
collection PubMed
description Bacterial wilt disease of tomato (Solanum lycopersicum L.), incited by Ralstonia solanacearum (Smith), is a serious agricultural problem in India. In this investigation, chemical mutagenic agents (NTG and HNO(2) treatment) and ultraviolet (UV) irradiation have been used to enhance the antagonistic property of Bacillus amyloliquefaciens DSBA-11 against R. solanacearum UTT-25 towards an effective management of tomato wilt disease. The investigation established the fact that maximum inhibition to R. solanacearum UTT-25 was exerted by the derivative strain MHNO(2)-20 treated with nitrous acid (HNO(2)) and then by the derivative strain MNTG-21 treated with NTG. The exertion was significantly higher than that of the parent B. amyloliquefaciens DSBA-11. These two potential derivatives viz. MNTG-21, MHNO(2)-20 along with MUV-19, and a wild derivative strain of B. amyloliquefaciens i.e.,DSBA-11 were selected for GC/MS analysis. Through this analysis 18 major compounds were detected. Among the compounds thus detected, the compound 3-isobutyl hexahydropyrrolo (1,2), pyrazine-1,4-dione (4.67%) was at maximum proportion in the variant MHNO(2)-20 at higher retention time (RT) of 43.19 s. Bio-efficacy assessment observed a record of minimum intensity (9.28%) in wilt disease and the highest bio-control (88.75%) in derivative strain MHNO(2)-20-treated plants after 30 days of inoculation. The derivative strain MHNO(2)-20, developed by treating B. amyloliquefaciens with nitrous acid (HNO(2)), was therefore found to have a higher bio-efficacy to control bacterial wilt disease of tomato under glasshouse conditions than a wild-type strain.
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spelling pubmed-103833712023-07-30 Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum Yadav, Dhananjay Kumar Devappa, Venkatappa Kashyap, Abhijeet Shankar Kumar, Narendra Rana, V. S. Sunita, Kumari Singh, Dinesh Microorganisms Article Bacterial wilt disease of tomato (Solanum lycopersicum L.), incited by Ralstonia solanacearum (Smith), is a serious agricultural problem in India. In this investigation, chemical mutagenic agents (NTG and HNO(2) treatment) and ultraviolet (UV) irradiation have been used to enhance the antagonistic property of Bacillus amyloliquefaciens DSBA-11 against R. solanacearum UTT-25 towards an effective management of tomato wilt disease. The investigation established the fact that maximum inhibition to R. solanacearum UTT-25 was exerted by the derivative strain MHNO(2)-20 treated with nitrous acid (HNO(2)) and then by the derivative strain MNTG-21 treated with NTG. The exertion was significantly higher than that of the parent B. amyloliquefaciens DSBA-11. These two potential derivatives viz. MNTG-21, MHNO(2)-20 along with MUV-19, and a wild derivative strain of B. amyloliquefaciens i.e.,DSBA-11 were selected for GC/MS analysis. Through this analysis 18 major compounds were detected. Among the compounds thus detected, the compound 3-isobutyl hexahydropyrrolo (1,2), pyrazine-1,4-dione (4.67%) was at maximum proportion in the variant MHNO(2)-20 at higher retention time (RT) of 43.19 s. Bio-efficacy assessment observed a record of minimum intensity (9.28%) in wilt disease and the highest bio-control (88.75%) in derivative strain MHNO(2)-20-treated plants after 30 days of inoculation. The derivative strain MHNO(2)-20, developed by treating B. amyloliquefaciens with nitrous acid (HNO(2)), was therefore found to have a higher bio-efficacy to control bacterial wilt disease of tomato under glasshouse conditions than a wild-type strain. MDPI 2023-07-12 /pmc/articles/PMC10383371/ /pubmed/37512962 http://dx.doi.org/10.3390/microorganisms11071790 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yadav, Dhananjay Kumar
Devappa, Venkatappa
Kashyap, Abhijeet Shankar
Kumar, Narendra
Rana, V. S.
Sunita, Kumari
Singh, Dinesh
Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title_full Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title_fullStr Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title_full_unstemmed Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title_short Boosting the Biocontrol Efficacy of Bacillus amyloliquefaciens DSBA-11 through Physical and Chemical Mutagens to Control Bacterial Wilt Disease of Tomato Caused by Ralstonia solanacearum
title_sort boosting the biocontrol efficacy of bacillus amyloliquefaciens dsba-11 through physical and chemical mutagens to control bacterial wilt disease of tomato caused by ralstonia solanacearum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383371/
https://www.ncbi.nlm.nih.gov/pubmed/37512962
http://dx.doi.org/10.3390/microorganisms11071790
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