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Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

RNA viruses tend to mutate during transmission and host infection, which is critical to viral adaptation and evolution. Tomato mosaic virus (ToMV) is a member of the genus Tobamovirus (family Virgaviridae) and an economically important virus with detrimental effects on tomatoes worldwide. Although t...

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Autores principales: Lyu, Jinfu, Yang, Yuanyuan, Sun, Xiaohui, Jiang, Shanshan, Hong, Hao, Zhu, Xiaoping, Liu, Yongguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383530/
https://www.ncbi.nlm.nih.gov/pubmed/37515303
http://dx.doi.org/10.3390/v15071617
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author Lyu, Jinfu
Yang, Yuanyuan
Sun, Xiaohui
Jiang, Shanshan
Hong, Hao
Zhu, Xiaoping
Liu, Yongguang
author_facet Lyu, Jinfu
Yang, Yuanyuan
Sun, Xiaohui
Jiang, Shanshan
Hong, Hao
Zhu, Xiaoping
Liu, Yongguang
author_sort Lyu, Jinfu
collection PubMed
description RNA viruses tend to mutate during transmission and host infection, which is critical to viral adaptation and evolution. Tomato mosaic virus (ToMV) is a member of the genus Tobamovirus (family Virgaviridae) and an economically important virus with detrimental effects on tomatoes worldwide. Although the ToMV gene sequences have been completed in China, their genetic diversity and population structure remain unclear. We collected 425 tomato samples from tomato-growing areas in three northern Chinese provinces 2016. Reverse transcription PCR results showed that the average incidence of the virus in the field samples was 67.15%, and ToMV was detected in all test areas. The analysis of ToMV single nucleotide polymorphisms in China showed that ToMV was evolutionarily conserved, and the variation in the whole genome was uneven. Pairwise identity analysis showed significant variability in genome sequences among ToMV strains with genomic nucleotide identities of 73.2–99.6%. The ToMV population in the northern Chinese provinces had purification and selection functions, which were beneficial in the evolution of the ToMV population. Although there has been some distribution of ToMV strains in China, the virus was generally stabilized as a uniform strain under the pressure of purification selection. Our findings show how to monitor the prevalent strains of ToMV and their virulence in China and provide useful information for its prevention and control.
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spelling pubmed-103835302023-07-30 Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces Lyu, Jinfu Yang, Yuanyuan Sun, Xiaohui Jiang, Shanshan Hong, Hao Zhu, Xiaoping Liu, Yongguang Viruses Article RNA viruses tend to mutate during transmission and host infection, which is critical to viral adaptation and evolution. Tomato mosaic virus (ToMV) is a member of the genus Tobamovirus (family Virgaviridae) and an economically important virus with detrimental effects on tomatoes worldwide. Although the ToMV gene sequences have been completed in China, their genetic diversity and population structure remain unclear. We collected 425 tomato samples from tomato-growing areas in three northern Chinese provinces 2016. Reverse transcription PCR results showed that the average incidence of the virus in the field samples was 67.15%, and ToMV was detected in all test areas. The analysis of ToMV single nucleotide polymorphisms in China showed that ToMV was evolutionarily conserved, and the variation in the whole genome was uneven. Pairwise identity analysis showed significant variability in genome sequences among ToMV strains with genomic nucleotide identities of 73.2–99.6%. The ToMV population in the northern Chinese provinces had purification and selection functions, which were beneficial in the evolution of the ToMV population. Although there has been some distribution of ToMV strains in China, the virus was generally stabilized as a uniform strain under the pressure of purification selection. Our findings show how to monitor the prevalent strains of ToMV and their virulence in China and provide useful information for its prevention and control. MDPI 2023-07-24 /pmc/articles/PMC10383530/ /pubmed/37515303 http://dx.doi.org/10.3390/v15071617 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lyu, Jinfu
Yang, Yuanyuan
Sun, Xiaohui
Jiang, Shanshan
Hong, Hao
Zhu, Xiaoping
Liu, Yongguang
Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title_full Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title_fullStr Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title_full_unstemmed Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title_short Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces
title_sort genetic variability and molecular evolution of tomato mosaic virus populations in three northern china provinces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383530/
https://www.ncbi.nlm.nih.gov/pubmed/37515303
http://dx.doi.org/10.3390/v15071617
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