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Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma

Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycopla...

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Autores principales: Gorina, Luisa G., Krylova, Natalya A., Rakovskaya, Irina V., Geppe, Natalia A., Gamova, Natalia A., Barkhatova, Olga I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383847/
https://www.ncbi.nlm.nih.gov/pubmed/37512855
http://dx.doi.org/10.3390/microorganisms11071683
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author Gorina, Luisa G.
Krylova, Natalya A.
Rakovskaya, Irina V.
Geppe, Natalia A.
Gamova, Natalia A.
Barkhatova, Olga I.
author_facet Gorina, Luisa G.
Krylova, Natalya A.
Rakovskaya, Irina V.
Geppe, Natalia A.
Gamova, Natalia A.
Barkhatova, Olga I.
author_sort Gorina, Luisa G.
collection PubMed
description Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycoplasma hominis (Mh) in children with asthma. In total, 205 children with asthma from 1 to 14 years were observed. The reaction of aggregate-hemagglutination (AHAA), the direct immunofluorescence reaction (DIF), the reaction of the polymerase chain reaction (PCR), and the culture method were used. In addition, 47 children were re-examined 1.5 months after the treatment of mycoplasma infection with azithromycin. The number of samples positive for antigens and DNA in the free state and in the structures of CICs significantly decreased. Then, 50 blood serum samples containing Mh antigens, and 50 samples containing Mpn antigens were analyzed by culture method. Mh was isolated in 21 (65.5%) of 32 samples containing DNA. Mpn was isolated from antigen-positive samples in nine cases. The presented data indicate the long-term persistence of antigens, and DNA of mycoplasma cells in the free state, in the structure of CICs, as well as in the form of “microcolonies”. A high level of CICs can be used to predict the course of the disease and the response to therapy.
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spelling pubmed-103838472023-07-30 Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma Gorina, Luisa G. Krylova, Natalya A. Rakovskaya, Irina V. Geppe, Natalia A. Gamova, Natalia A. Barkhatova, Olga I. Microorganisms Communication Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycoplasma hominis (Mh) in children with asthma. In total, 205 children with asthma from 1 to 14 years were observed. The reaction of aggregate-hemagglutination (AHAA), the direct immunofluorescence reaction (DIF), the reaction of the polymerase chain reaction (PCR), and the culture method were used. In addition, 47 children were re-examined 1.5 months after the treatment of mycoplasma infection with azithromycin. The number of samples positive for antigens and DNA in the free state and in the structures of CICs significantly decreased. Then, 50 blood serum samples containing Mh antigens, and 50 samples containing Mpn antigens were analyzed by culture method. Mh was isolated in 21 (65.5%) of 32 samples containing DNA. Mpn was isolated from antigen-positive samples in nine cases. The presented data indicate the long-term persistence of antigens, and DNA of mycoplasma cells in the free state, in the structure of CICs, as well as in the form of “microcolonies”. A high level of CICs can be used to predict the course of the disease and the response to therapy. MDPI 2023-06-28 /pmc/articles/PMC10383847/ /pubmed/37512855 http://dx.doi.org/10.3390/microorganisms11071683 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gorina, Luisa G.
Krylova, Natalya A.
Rakovskaya, Irina V.
Geppe, Natalia A.
Gamova, Natalia A.
Barkhatova, Olga I.
Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title_full Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title_fullStr Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title_full_unstemmed Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title_short Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
title_sort mechanisms of long-term persistence of mycoplasmas in children with asthma
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383847/
https://www.ncbi.nlm.nih.gov/pubmed/37512855
http://dx.doi.org/10.3390/microorganisms11071683
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