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Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma
Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycopla...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383847/ https://www.ncbi.nlm.nih.gov/pubmed/37512855 http://dx.doi.org/10.3390/microorganisms11071683 |
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author | Gorina, Luisa G. Krylova, Natalya A. Rakovskaya, Irina V. Geppe, Natalia A. Gamova, Natalia A. Barkhatova, Olga I. |
author_facet | Gorina, Luisa G. Krylova, Natalya A. Rakovskaya, Irina V. Geppe, Natalia A. Gamova, Natalia A. Barkhatova, Olga I. |
author_sort | Gorina, Luisa G. |
collection | PubMed |
description | Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycoplasma hominis (Mh) in children with asthma. In total, 205 children with asthma from 1 to 14 years were observed. The reaction of aggregate-hemagglutination (AHAA), the direct immunofluorescence reaction (DIF), the reaction of the polymerase chain reaction (PCR), and the culture method were used. In addition, 47 children were re-examined 1.5 months after the treatment of mycoplasma infection with azithromycin. The number of samples positive for antigens and DNA in the free state and in the structures of CICs significantly decreased. Then, 50 blood serum samples containing Mh antigens, and 50 samples containing Mpn antigens were analyzed by culture method. Mh was isolated in 21 (65.5%) of 32 samples containing DNA. Mpn was isolated from antigen-positive samples in nine cases. The presented data indicate the long-term persistence of antigens, and DNA of mycoplasma cells in the free state, in the structure of CICs, as well as in the form of “microcolonies”. A high level of CICs can be used to predict the course of the disease and the response to therapy. |
format | Online Article Text |
id | pubmed-10383847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103838472023-07-30 Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma Gorina, Luisa G. Krylova, Natalya A. Rakovskaya, Irina V. Geppe, Natalia A. Gamova, Natalia A. Barkhatova, Olga I. Microorganisms Communication Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of Mycoplasma pneumoniae (Mpn) and Mycoplasma hominis (Mh) in children with asthma. In total, 205 children with asthma from 1 to 14 years were observed. The reaction of aggregate-hemagglutination (AHAA), the direct immunofluorescence reaction (DIF), the reaction of the polymerase chain reaction (PCR), and the culture method were used. In addition, 47 children were re-examined 1.5 months after the treatment of mycoplasma infection with azithromycin. The number of samples positive for antigens and DNA in the free state and in the structures of CICs significantly decreased. Then, 50 blood serum samples containing Mh antigens, and 50 samples containing Mpn antigens were analyzed by culture method. Mh was isolated in 21 (65.5%) of 32 samples containing DNA. Mpn was isolated from antigen-positive samples in nine cases. The presented data indicate the long-term persistence of antigens, and DNA of mycoplasma cells in the free state, in the structure of CICs, as well as in the form of “microcolonies”. A high level of CICs can be used to predict the course of the disease and the response to therapy. MDPI 2023-06-28 /pmc/articles/PMC10383847/ /pubmed/37512855 http://dx.doi.org/10.3390/microorganisms11071683 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Gorina, Luisa G. Krylova, Natalya A. Rakovskaya, Irina V. Geppe, Natalia A. Gamova, Natalia A. Barkhatova, Olga I. Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title | Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title_full | Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title_fullStr | Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title_full_unstemmed | Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title_short | Mechanisms of Long-Term Persistence of Mycoplasmas in Children with Asthma |
title_sort | mechanisms of long-term persistence of mycoplasmas in children with asthma |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383847/ https://www.ncbi.nlm.nih.gov/pubmed/37512855 http://dx.doi.org/10.3390/microorganisms11071683 |
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