Cargando…
n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in ch...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383889/ https://www.ncbi.nlm.nih.gov/pubmed/37513526 http://dx.doi.org/10.3390/nu15143108 |
_version_ | 1785081021924900864 |
---|---|
author | Gortan Cappellari, Gianluca Aleksova, Aneta Dal Ferro, Matteo Cannatà, Antonio Semolic, Annamaria Guarnaccia, Alberto Zanetti, Michela Giacca, Mauro Sinagra, Gianfranco Barazzoni, Rocco |
author_facet | Gortan Cappellari, Gianluca Aleksova, Aneta Dal Ferro, Matteo Cannatà, Antonio Semolic, Annamaria Guarnaccia, Alberto Zanetti, Michela Giacca, Mauro Sinagra, Gianfranco Barazzoni, Rocco |
author_sort | Gortan Cappellari, Gianluca |
collection | PubMed |
description | Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in chronic heart failure (CHF). We hypothesized that 8-week n-3-PUFA isocaloric partial dietary replacement (Fat = 5.5% total cal; EPA + DHA = 27% total fat) normalizes gastrocnemius muscle (GM) mitochondrial dynamics regulators, mitochondrial and tissue pro-oxidative changes, and catabolic derangements, resulting in preserved GM mass in rodent CHF [Myocardial infarction (MI)-induced CHF by coronary artery ligation, left-ventricular ejection fraction <50%]. Results: Compared to control animals (Sham), CHF had a higher GM mitochondrial fission-fusion protein ratio, with low ATP and high ROS production, pro-inflammatory changes, and low insulin signalling. n-3-PUFA normalized all mitochondrial derangements and the pro-oxidative state (oxidized to total glutathione ratio), associated with normalized GM cytokine profile, and enhanced muscle-anabolic insulin signalling and prevention of CHF-induced GM weight loss (all p < 0.05 vs. CHF and p = NS vs. S). Conclusions: n-3-PUFA isocaloric partial dietary replacement for 8 weeks normalizes CHF-induced derangements of muscle mitochondrial dynamics regulators, ROS production and function. n-3-PUFA mitochondrial effects result in preserved skeletal muscle mass, with potential to improve major patient outcomes in clinical settings. |
format | Online Article Text |
id | pubmed-10383889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103838892023-07-30 n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure Gortan Cappellari, Gianluca Aleksova, Aneta Dal Ferro, Matteo Cannatà, Antonio Semolic, Annamaria Guarnaccia, Alberto Zanetti, Michela Giacca, Mauro Sinagra, Gianfranco Barazzoni, Rocco Nutrients Article Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in chronic heart failure (CHF). We hypothesized that 8-week n-3-PUFA isocaloric partial dietary replacement (Fat = 5.5% total cal; EPA + DHA = 27% total fat) normalizes gastrocnemius muscle (GM) mitochondrial dynamics regulators, mitochondrial and tissue pro-oxidative changes, and catabolic derangements, resulting in preserved GM mass in rodent CHF [Myocardial infarction (MI)-induced CHF by coronary artery ligation, left-ventricular ejection fraction <50%]. Results: Compared to control animals (Sham), CHF had a higher GM mitochondrial fission-fusion protein ratio, with low ATP and high ROS production, pro-inflammatory changes, and low insulin signalling. n-3-PUFA normalized all mitochondrial derangements and the pro-oxidative state (oxidized to total glutathione ratio), associated with normalized GM cytokine profile, and enhanced muscle-anabolic insulin signalling and prevention of CHF-induced GM weight loss (all p < 0.05 vs. CHF and p = NS vs. S). Conclusions: n-3-PUFA isocaloric partial dietary replacement for 8 weeks normalizes CHF-induced derangements of muscle mitochondrial dynamics regulators, ROS production and function. n-3-PUFA mitochondrial effects result in preserved skeletal muscle mass, with potential to improve major patient outcomes in clinical settings. MDPI 2023-07-11 /pmc/articles/PMC10383889/ /pubmed/37513526 http://dx.doi.org/10.3390/nu15143108 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gortan Cappellari, Gianluca Aleksova, Aneta Dal Ferro, Matteo Cannatà, Antonio Semolic, Annamaria Guarnaccia, Alberto Zanetti, Michela Giacca, Mauro Sinagra, Gianfranco Barazzoni, Rocco n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title | n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title_full | n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title_fullStr | n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title_full_unstemmed | n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title_short | n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure |
title_sort | n-3 pufa-enriched diet preserves skeletal muscle mitochondrial function and redox state and prevents muscle mass loss in mice with chronic heart failure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383889/ https://www.ncbi.nlm.nih.gov/pubmed/37513526 http://dx.doi.org/10.3390/nu15143108 |
work_keys_str_mv | AT gortancappellarigianluca n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT aleksovaaneta n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT dalferromatteo n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT cannataantonio n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT semolicannamaria n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT guarnacciaalberto n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT zanettimichela n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT giaccamauro n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT sinagragianfranco n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure AT barazzonirocco n3pufaenricheddietpreservesskeletalmusclemitochondrialfunctionandredoxstateandpreventsmusclemasslossinmicewithchronicheartfailure |