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n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure

Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in ch...

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Autores principales: Gortan Cappellari, Gianluca, Aleksova, Aneta, Dal Ferro, Matteo, Cannatà, Antonio, Semolic, Annamaria, Guarnaccia, Alberto, Zanetti, Michela, Giacca, Mauro, Sinagra, Gianfranco, Barazzoni, Rocco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383889/
https://www.ncbi.nlm.nih.gov/pubmed/37513526
http://dx.doi.org/10.3390/nu15143108
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author Gortan Cappellari, Gianluca
Aleksova, Aneta
Dal Ferro, Matteo
Cannatà, Antonio
Semolic, Annamaria
Guarnaccia, Alberto
Zanetti, Michela
Giacca, Mauro
Sinagra, Gianfranco
Barazzoni, Rocco
author_facet Gortan Cappellari, Gianluca
Aleksova, Aneta
Dal Ferro, Matteo
Cannatà, Antonio
Semolic, Annamaria
Guarnaccia, Alberto
Zanetti, Michela
Giacca, Mauro
Sinagra, Gianfranco
Barazzoni, Rocco
author_sort Gortan Cappellari, Gianluca
collection PubMed
description Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in chronic heart failure (CHF). We hypothesized that 8-week n-3-PUFA isocaloric partial dietary replacement (Fat = 5.5% total cal; EPA + DHA = 27% total fat) normalizes gastrocnemius muscle (GM) mitochondrial dynamics regulators, mitochondrial and tissue pro-oxidative changes, and catabolic derangements, resulting in preserved GM mass in rodent CHF [Myocardial infarction (MI)-induced CHF by coronary artery ligation, left-ventricular ejection fraction <50%]. Results: Compared to control animals (Sham), CHF had a higher GM mitochondrial fission-fusion protein ratio, with low ATP and high ROS production, pro-inflammatory changes, and low insulin signalling. n-3-PUFA normalized all mitochondrial derangements and the pro-oxidative state (oxidized to total glutathione ratio), associated with normalized GM cytokine profile, and enhanced muscle-anabolic insulin signalling and prevention of CHF-induced GM weight loss (all p < 0.05 vs. CHF and p = NS vs. S). Conclusions: n-3-PUFA isocaloric partial dietary replacement for 8 weeks normalizes CHF-induced derangements of muscle mitochondrial dynamics regulators, ROS production and function. n-3-PUFA mitochondrial effects result in preserved skeletal muscle mass, with potential to improve major patient outcomes in clinical settings.
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spelling pubmed-103838892023-07-30 n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure Gortan Cappellari, Gianluca Aleksova, Aneta Dal Ferro, Matteo Cannatà, Antonio Semolic, Annamaria Guarnaccia, Alberto Zanetti, Michela Giacca, Mauro Sinagra, Gianfranco Barazzoni, Rocco Nutrients Article Rationale and Methods: Skeletal muscle derangements, potentially including mitochondrial dysfunction with altered mitochondrial dynamics and high reactive oxygen species (ROS) generation, may lead to protein catabolism and muscle wasting, resulting in low exercise capacity and reduced survival in chronic heart failure (CHF). We hypothesized that 8-week n-3-PUFA isocaloric partial dietary replacement (Fat = 5.5% total cal; EPA + DHA = 27% total fat) normalizes gastrocnemius muscle (GM) mitochondrial dynamics regulators, mitochondrial and tissue pro-oxidative changes, and catabolic derangements, resulting in preserved GM mass in rodent CHF [Myocardial infarction (MI)-induced CHF by coronary artery ligation, left-ventricular ejection fraction <50%]. Results: Compared to control animals (Sham), CHF had a higher GM mitochondrial fission-fusion protein ratio, with low ATP and high ROS production, pro-inflammatory changes, and low insulin signalling. n-3-PUFA normalized all mitochondrial derangements and the pro-oxidative state (oxidized to total glutathione ratio), associated with normalized GM cytokine profile, and enhanced muscle-anabolic insulin signalling and prevention of CHF-induced GM weight loss (all p < 0.05 vs. CHF and p = NS vs. S). Conclusions: n-3-PUFA isocaloric partial dietary replacement for 8 weeks normalizes CHF-induced derangements of muscle mitochondrial dynamics regulators, ROS production and function. n-3-PUFA mitochondrial effects result in preserved skeletal muscle mass, with potential to improve major patient outcomes in clinical settings. MDPI 2023-07-11 /pmc/articles/PMC10383889/ /pubmed/37513526 http://dx.doi.org/10.3390/nu15143108 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gortan Cappellari, Gianluca
Aleksova, Aneta
Dal Ferro, Matteo
Cannatà, Antonio
Semolic, Annamaria
Guarnaccia, Alberto
Zanetti, Michela
Giacca, Mauro
Sinagra, Gianfranco
Barazzoni, Rocco
n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title_full n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title_fullStr n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title_full_unstemmed n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title_short n-3 PUFA-Enriched Diet Preserves Skeletal Muscle Mitochondrial Function and Redox State and Prevents Muscle Mass Loss in Mice with Chronic Heart Failure
title_sort n-3 pufa-enriched diet preserves skeletal muscle mitochondrial function and redox state and prevents muscle mass loss in mice with chronic heart failure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383889/
https://www.ncbi.nlm.nih.gov/pubmed/37513526
http://dx.doi.org/10.3390/nu15143108
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