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Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc

Infections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is...

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Autores principales: Manso, Tamara, Lores, Marta, Rama, José Luis R., Villarino, Rosa-Antía, Calvo, Lorena G., Castillo, Aly, Celeiro, María, de Miguel, Trinidad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383933/
https://www.ncbi.nlm.nih.gov/pubmed/37513862
http://dx.doi.org/10.3390/ph16070950
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author Manso, Tamara
Lores, Marta
Rama, José Luis R.
Villarino, Rosa-Antía
Calvo, Lorena G.
Castillo, Aly
Celeiro, María
de Miguel, Trinidad
author_facet Manso, Tamara
Lores, Marta
Rama, José Luis R.
Villarino, Rosa-Antía
Calvo, Lorena G.
Castillo, Aly
Celeiro, María
de Miguel, Trinidad
author_sort Manso, Tamara
collection PubMed
description Infections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is, therefore, urgent to find alternatives to treat infections caused by multidrug-resistant bacteria. This study aimed at exploring a possible therapeutic alternative in the fight against antibiotic resistance. Based on the known antibacterial capacity of polyphenols, we tested the antimicrobial activity of a polyphenolic extract of Albariño white grape marc on clinical strains since research on such bacteria has been very scarce until now. First, the extract was obtained using a medium-scale ambient temperature (MSAT) system, which is an efficient and sustainable extractive method. The determinations of the polyphenolic content of the extract and its antioxidant capacity showed good results. Using chromatographic and mass spectrometric tools, 13 remarkable polyphenols were detected in the extract. The antibacterial activity of our grape marc extract against nineteen clinical strain isolates, some of which are multidrug-resistant, was evaluated by means of the calculation of half of the maximum inhibitory concentration (IC50) and the value of the minimum bactericidal concentrations (MBCs). In conclusion, the extract showed effectiveness against all clinical strains tested, regardless of their level of antibiotic resistance, and shows promise in the fight against antibiotic resistance.
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spelling pubmed-103839332023-07-30 Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc Manso, Tamara Lores, Marta Rama, José Luis R. Villarino, Rosa-Antía Calvo, Lorena G. Castillo, Aly Celeiro, María de Miguel, Trinidad Pharmaceuticals (Basel) Article Infections caused by multidrug-resistant bacteria are becoming increasingly frequent and sometimes difficult to treat due to the limited number of antibiotics active against them. In addition, they can spread between countries and/or continents, which is a problem of great relevance worldwide. It is, therefore, urgent to find alternatives to treat infections caused by multidrug-resistant bacteria. This study aimed at exploring a possible therapeutic alternative in the fight against antibiotic resistance. Based on the known antibacterial capacity of polyphenols, we tested the antimicrobial activity of a polyphenolic extract of Albariño white grape marc on clinical strains since research on such bacteria has been very scarce until now. First, the extract was obtained using a medium-scale ambient temperature (MSAT) system, which is an efficient and sustainable extractive method. The determinations of the polyphenolic content of the extract and its antioxidant capacity showed good results. Using chromatographic and mass spectrometric tools, 13 remarkable polyphenols were detected in the extract. The antibacterial activity of our grape marc extract against nineteen clinical strain isolates, some of which are multidrug-resistant, was evaluated by means of the calculation of half of the maximum inhibitory concentration (IC50) and the value of the minimum bactericidal concentrations (MBCs). In conclusion, the extract showed effectiveness against all clinical strains tested, regardless of their level of antibiotic resistance, and shows promise in the fight against antibiotic resistance. MDPI 2023-07-01 /pmc/articles/PMC10383933/ /pubmed/37513862 http://dx.doi.org/10.3390/ph16070950 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Manso, Tamara
Lores, Marta
Rama, José Luis R.
Villarino, Rosa-Antía
Calvo, Lorena G.
Castillo, Aly
Celeiro, María
de Miguel, Trinidad
Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title_full Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title_fullStr Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title_full_unstemmed Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title_short Antibacterial Activity against Clinical Strains of a Natural Polyphenolic Extract from Albariño White Grape Marc
title_sort antibacterial activity against clinical strains of a natural polyphenolic extract from albariño white grape marc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383933/
https://www.ncbi.nlm.nih.gov/pubmed/37513862
http://dx.doi.org/10.3390/ph16070950
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