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LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS

Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of c...

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Autores principales: Mendoza-Arroyo, Belen, Rosales-Hernández, Martha Cecilia, Pacheco-Yépez, Judith, Rivera-Antonio, Astrid Mayleth, Márquez-Flores, Yazmín Karina, Cárdenas-Jaramillo, Luz María, Reséndiz-Albor, Aldo Arturo, Arciniega-Martínez, Ivonne Maciel, Cruz-Hernández, Teresita Rocío, Abarca-Rojano, Edgar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384056/
https://www.ncbi.nlm.nih.gov/pubmed/37512550
http://dx.doi.org/10.3390/metabo13070843
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author Mendoza-Arroyo, Belen
Rosales-Hernández, Martha Cecilia
Pacheco-Yépez, Judith
Rivera-Antonio, Astrid Mayleth
Márquez-Flores, Yazmín Karina
Cárdenas-Jaramillo, Luz María
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Cruz-Hernández, Teresita Rocío
Abarca-Rojano, Edgar
author_facet Mendoza-Arroyo, Belen
Rosales-Hernández, Martha Cecilia
Pacheco-Yépez, Judith
Rivera-Antonio, Astrid Mayleth
Márquez-Flores, Yazmín Karina
Cárdenas-Jaramillo, Luz María
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Cruz-Hernández, Teresita Rocío
Abarca-Rojano, Edgar
author_sort Mendoza-Arroyo, Belen
collection PubMed
description Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of colitis was used because it contains a complex inflammatory milieu of polymorphonuclear cells (PMN) and lymphocytes infiltrating the lamina propria. The aim of the present study was to investigate six dehydrogenases and their respective adaptations in the tissue microenvironment by quantifying enzymatic activities measured under substrate saturation conditions in epithelial cells and leukocytes from the lamina propria of rats exposed to TNBS. Our results show that in the TNBS group, an increased DAI score was observed due to the presence of haemorrhagic and necrotic areas in the colon. In addition, the activities of G6PDH and GADH enzymes were significantly decreased in the epithelium in contrast to the increased activity of these enzymes and increased lactate mediated by the LDH-A enzyme in leukocytes in the lamina propria of the colon. Over the past years, evidence has emerged illustrating how metabolism supports aspect of cellular function and how a metabolic reprogramming can drive cell differentiation and fate. Our findings show a metabolic reprogramming in colonic lamina propria leukocytes that could be supported by increased superoxide anion.
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spelling pubmed-103840562023-07-30 LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS Mendoza-Arroyo, Belen Rosales-Hernández, Martha Cecilia Pacheco-Yépez, Judith Rivera-Antonio, Astrid Mayleth Márquez-Flores, Yazmín Karina Cárdenas-Jaramillo, Luz María Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Cruz-Hernández, Teresita Rocío Abarca-Rojano, Edgar Metabolites Article Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of colitis was used because it contains a complex inflammatory milieu of polymorphonuclear cells (PMN) and lymphocytes infiltrating the lamina propria. The aim of the present study was to investigate six dehydrogenases and their respective adaptations in the tissue microenvironment by quantifying enzymatic activities measured under substrate saturation conditions in epithelial cells and leukocytes from the lamina propria of rats exposed to TNBS. Our results show that in the TNBS group, an increased DAI score was observed due to the presence of haemorrhagic and necrotic areas in the colon. In addition, the activities of G6PDH and GADH enzymes were significantly decreased in the epithelium in contrast to the increased activity of these enzymes and increased lactate mediated by the LDH-A enzyme in leukocytes in the lamina propria of the colon. Over the past years, evidence has emerged illustrating how metabolism supports aspect of cellular function and how a metabolic reprogramming can drive cell differentiation and fate. Our findings show a metabolic reprogramming in colonic lamina propria leukocytes that could be supported by increased superoxide anion. MDPI 2023-07-12 /pmc/articles/PMC10384056/ /pubmed/37512550 http://dx.doi.org/10.3390/metabo13070843 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mendoza-Arroyo, Belen
Rosales-Hernández, Martha Cecilia
Pacheco-Yépez, Judith
Rivera-Antonio, Astrid Mayleth
Márquez-Flores, Yazmín Karina
Cárdenas-Jaramillo, Luz María
Reséndiz-Albor, Aldo Arturo
Arciniega-Martínez, Ivonne Maciel
Cruz-Hernández, Teresita Rocío
Abarca-Rojano, Edgar
LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title_full LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title_fullStr LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title_full_unstemmed LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title_short LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
title_sort ldh-a promotes metabolic rewiring in leucocytes from the intestine of rats treated with tnbs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384056/
https://www.ncbi.nlm.nih.gov/pubmed/37512550
http://dx.doi.org/10.3390/metabo13070843
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