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LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS
Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of c...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384056/ https://www.ncbi.nlm.nih.gov/pubmed/37512550 http://dx.doi.org/10.3390/metabo13070843 |
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author | Mendoza-Arroyo, Belen Rosales-Hernández, Martha Cecilia Pacheco-Yépez, Judith Rivera-Antonio, Astrid Mayleth Márquez-Flores, Yazmín Karina Cárdenas-Jaramillo, Luz María Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Cruz-Hernández, Teresita Rocío Abarca-Rojano, Edgar |
author_facet | Mendoza-Arroyo, Belen Rosales-Hernández, Martha Cecilia Pacheco-Yépez, Judith Rivera-Antonio, Astrid Mayleth Márquez-Flores, Yazmín Karina Cárdenas-Jaramillo, Luz María Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Cruz-Hernández, Teresita Rocío Abarca-Rojano, Edgar |
author_sort | Mendoza-Arroyo, Belen |
collection | PubMed |
description | Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of colitis was used because it contains a complex inflammatory milieu of polymorphonuclear cells (PMN) and lymphocytes infiltrating the lamina propria. The aim of the present study was to investigate six dehydrogenases and their respective adaptations in the tissue microenvironment by quantifying enzymatic activities measured under substrate saturation conditions in epithelial cells and leukocytes from the lamina propria of rats exposed to TNBS. Our results show that in the TNBS group, an increased DAI score was observed due to the presence of haemorrhagic and necrotic areas in the colon. In addition, the activities of G6PDH and GADH enzymes were significantly decreased in the epithelium in contrast to the increased activity of these enzymes and increased lactate mediated by the LDH-A enzyme in leukocytes in the lamina propria of the colon. Over the past years, evidence has emerged illustrating how metabolism supports aspect of cellular function and how a metabolic reprogramming can drive cell differentiation and fate. Our findings show a metabolic reprogramming in colonic lamina propria leukocytes that could be supported by increased superoxide anion. |
format | Online Article Text |
id | pubmed-10384056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103840562023-07-30 LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS Mendoza-Arroyo, Belen Rosales-Hernández, Martha Cecilia Pacheco-Yépez, Judith Rivera-Antonio, Astrid Mayleth Márquez-Flores, Yazmín Karina Cárdenas-Jaramillo, Luz María Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Cruz-Hernández, Teresita Rocío Abarca-Rojano, Edgar Metabolites Article Although the aetiology of inflammatory bowel diseases (IBDs) is still unknown, one of their main characteristics is that the immune system chronically affects the permeability of the intestinal lamina propria, in turn altering the composition of the microbiota. In this study, the TNBS rat model of colitis was used because it contains a complex inflammatory milieu of polymorphonuclear cells (PMN) and lymphocytes infiltrating the lamina propria. The aim of the present study was to investigate six dehydrogenases and their respective adaptations in the tissue microenvironment by quantifying enzymatic activities measured under substrate saturation conditions in epithelial cells and leukocytes from the lamina propria of rats exposed to TNBS. Our results show that in the TNBS group, an increased DAI score was observed due to the presence of haemorrhagic and necrotic areas in the colon. In addition, the activities of G6PDH and GADH enzymes were significantly decreased in the epithelium in contrast to the increased activity of these enzymes and increased lactate mediated by the LDH-A enzyme in leukocytes in the lamina propria of the colon. Over the past years, evidence has emerged illustrating how metabolism supports aspect of cellular function and how a metabolic reprogramming can drive cell differentiation and fate. Our findings show a metabolic reprogramming in colonic lamina propria leukocytes that could be supported by increased superoxide anion. MDPI 2023-07-12 /pmc/articles/PMC10384056/ /pubmed/37512550 http://dx.doi.org/10.3390/metabo13070843 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mendoza-Arroyo, Belen Rosales-Hernández, Martha Cecilia Pacheco-Yépez, Judith Rivera-Antonio, Astrid Mayleth Márquez-Flores, Yazmín Karina Cárdenas-Jaramillo, Luz María Reséndiz-Albor, Aldo Arturo Arciniega-Martínez, Ivonne Maciel Cruz-Hernández, Teresita Rocío Abarca-Rojano, Edgar LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title | LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title_full | LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title_fullStr | LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title_full_unstemmed | LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title_short | LDH-A Promotes Metabolic Rewiring in Leucocytes from the Intestine of Rats Treated with TNBS |
title_sort | ldh-a promotes metabolic rewiring in leucocytes from the intestine of rats treated with tnbs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384056/ https://www.ncbi.nlm.nih.gov/pubmed/37512550 http://dx.doi.org/10.3390/metabo13070843 |
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