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Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years
The situation of limited data concerning the response to COVID-19 mRNA vaccinations in immunocom-promised children hinders evidence-based recommendations. This prospective observational study investigated humoral and T cell responses after primary BNT162b2 vaccination in secondary immunocompromised...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384144/ https://www.ncbi.nlm.nih.gov/pubmed/37515239 http://dx.doi.org/10.3390/v15071553 |
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author | Lalia, Jasmin K. Schild, Raphael Lütgehetmann, Marc Dunay, Gabor A. Kallinich, Tilmann Kobbe, Robin Massoud, Mona Oh, Jun Pietzsch, Leonora Schulze-Sturm, Ulf Schuetz, Catharina Sibbertsen, Freya Speth, Fabian Thieme, Sebastian Witkowski, Mario Berner, Reinhard Muntau, Ania C. Gersting, Søren W. Toepfner, Nicole Pagel, Julia Paul, Kevin |
author_facet | Lalia, Jasmin K. Schild, Raphael Lütgehetmann, Marc Dunay, Gabor A. Kallinich, Tilmann Kobbe, Robin Massoud, Mona Oh, Jun Pietzsch, Leonora Schulze-Sturm, Ulf Schuetz, Catharina Sibbertsen, Freya Speth, Fabian Thieme, Sebastian Witkowski, Mario Berner, Reinhard Muntau, Ania C. Gersting, Søren W. Toepfner, Nicole Pagel, Julia Paul, Kevin |
author_sort | Lalia, Jasmin K. |
collection | PubMed |
description | The situation of limited data concerning the response to COVID-19 mRNA vaccinations in immunocom-promised children hinders evidence-based recommendations. This prospective observational study investigated humoral and T cell responses after primary BNT162b2 vaccination in secondary immunocompromised and healthy children aged 5–11 years. Participants were categorized as: children after kidney transplantation (KTx, n = 9), proteinuric glomerulonephritis (GN, n = 4) and healthy children (controls, n = 8). Expression of activation-induced markers and cytokine secretion were determined to quantify the T cell response from PBMCs stimulated with peptide pools covering the spike glycoprotein of SARS-CoV-2 Wuhan Hu-1 and Omicron BA.5. Antibodies against SARS-CoV-2 spike receptor-binding domain were quantified in serum. Seroconversion was detected in 56% of KTx patients and in 100% of the GN patients and controls. Titer levels were significantly higher in GN patients and controls than in KTx patients. In Ktx patients, the humoral response increased after a third immunization. No differences in the frequency of antigen-specific CD4+ and CD8+ T cells between all groups were observed. T cells showed a predominant anti-viral capacity in their secreted cytokines; however, this capacity was reduced in KTx patients. This study provides missing evidence concerning the humoral and T cell response in immunocompromised children after COVID-19 vaccination. |
format | Online Article Text |
id | pubmed-10384144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103841442023-07-30 Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years Lalia, Jasmin K. Schild, Raphael Lütgehetmann, Marc Dunay, Gabor A. Kallinich, Tilmann Kobbe, Robin Massoud, Mona Oh, Jun Pietzsch, Leonora Schulze-Sturm, Ulf Schuetz, Catharina Sibbertsen, Freya Speth, Fabian Thieme, Sebastian Witkowski, Mario Berner, Reinhard Muntau, Ania C. Gersting, Søren W. Toepfner, Nicole Pagel, Julia Paul, Kevin Viruses Article The situation of limited data concerning the response to COVID-19 mRNA vaccinations in immunocom-promised children hinders evidence-based recommendations. This prospective observational study investigated humoral and T cell responses after primary BNT162b2 vaccination in secondary immunocompromised and healthy children aged 5–11 years. Participants were categorized as: children after kidney transplantation (KTx, n = 9), proteinuric glomerulonephritis (GN, n = 4) and healthy children (controls, n = 8). Expression of activation-induced markers and cytokine secretion were determined to quantify the T cell response from PBMCs stimulated with peptide pools covering the spike glycoprotein of SARS-CoV-2 Wuhan Hu-1 and Omicron BA.5. Antibodies against SARS-CoV-2 spike receptor-binding domain were quantified in serum. Seroconversion was detected in 56% of KTx patients and in 100% of the GN patients and controls. Titer levels were significantly higher in GN patients and controls than in KTx patients. In Ktx patients, the humoral response increased after a third immunization. No differences in the frequency of antigen-specific CD4+ and CD8+ T cells between all groups were observed. T cells showed a predominant anti-viral capacity in their secreted cytokines; however, this capacity was reduced in KTx patients. This study provides missing evidence concerning the humoral and T cell response in immunocompromised children after COVID-19 vaccination. MDPI 2023-07-14 /pmc/articles/PMC10384144/ /pubmed/37515239 http://dx.doi.org/10.3390/v15071553 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lalia, Jasmin K. Schild, Raphael Lütgehetmann, Marc Dunay, Gabor A. Kallinich, Tilmann Kobbe, Robin Massoud, Mona Oh, Jun Pietzsch, Leonora Schulze-Sturm, Ulf Schuetz, Catharina Sibbertsen, Freya Speth, Fabian Thieme, Sebastian Witkowski, Mario Berner, Reinhard Muntau, Ania C. Gersting, Søren W. Toepfner, Nicole Pagel, Julia Paul, Kevin Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title | Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title_full | Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title_fullStr | Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title_full_unstemmed | Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title_short | Reduced Humoral and Cellular Immune Response to Primary COVID-19 mRNA Vaccination in Kidney Transplanted Children Aged 5–11 Years |
title_sort | reduced humoral and cellular immune response to primary covid-19 mrna vaccination in kidney transplanted children aged 5–11 years |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384144/ https://www.ncbi.nlm.nih.gov/pubmed/37515239 http://dx.doi.org/10.3390/v15071553 |
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