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Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization

The generation of a specific long-term immune response to SARS-CoV-2 is considered important for protection against COVID-19 infection and disease. Memory B cells, responsible for the generation of antibody-producing plasmablasts upon a new antigen encounter, play an important role in this process....

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Autores principales: Verheul, Marije K., Nijhof, Kim H., de Zeeuw-Brouwer, Mary-lène, Duijm, Geraly, ten Hulscher, Hinke, de Rond, Lia, Beckers, Lisa, Eggink, Dirk, van Tol, Sophie, Reimerink, Johan, Boer, Mardi, van Beek, Josine, Rots, Nynke, van Binnendijk, Rob, Buisman, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384172/
https://www.ncbi.nlm.nih.gov/pubmed/37515012
http://dx.doi.org/10.3390/vaccines11071196
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author Verheul, Marije K.
Nijhof, Kim H.
de Zeeuw-Brouwer, Mary-lène
Duijm, Geraly
ten Hulscher, Hinke
de Rond, Lia
Beckers, Lisa
Eggink, Dirk
van Tol, Sophie
Reimerink, Johan
Boer, Mardi
van Beek, Josine
Rots, Nynke
van Binnendijk, Rob
Buisman, Anne-Marie
author_facet Verheul, Marije K.
Nijhof, Kim H.
de Zeeuw-Brouwer, Mary-lène
Duijm, Geraly
ten Hulscher, Hinke
de Rond, Lia
Beckers, Lisa
Eggink, Dirk
van Tol, Sophie
Reimerink, Johan
Boer, Mardi
van Beek, Josine
Rots, Nynke
van Binnendijk, Rob
Buisman, Anne-Marie
author_sort Verheul, Marije K.
collection PubMed
description The generation of a specific long-term immune response to SARS-CoV-2 is considered important for protection against COVID-19 infection and disease. Memory B cells, responsible for the generation of antibody-producing plasmablasts upon a new antigen encounter, play an important role in this process. Therefore, the induction of memory B cell responses after primary and booster SARS-CoV-2 immunizations was investigated in the general population with an emphasis on older adults. Participants, 20–99 years of age, due to receive the mRNA-1273 or BNT162b2 SARS-CoV-2 vaccine were included in the current study. Specific memory B cells were determined by ex vivo ELISpot assays. In a subset of participants, antibody levels, avidity, and virus neutralization capacity were compared to memory B cell responses. Memory B cells specific for both Spike S1 and receptor-binding domain (RBD) were detected in the majority of participants following the primary immunization series. However, a proportion of predominantly older adults showed low frequencies of specific memory B cells. Booster vaccination resulted in a large increase in the frequencies of S1- and RBD-specific memory B cells also for those in which low memory B cell frequencies were detected after the primary series. These data show that booster immunization is important for the generation of a memory B cell response, as a subset of older adults shows a suboptimal response to the primary SARS-CoV-2 immunization series. It is anticipated that these memory B cells will play a significant role in the immune response following viral re-exposure.
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spelling pubmed-103841722023-07-30 Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization Verheul, Marije K. Nijhof, Kim H. de Zeeuw-Brouwer, Mary-lène Duijm, Geraly ten Hulscher, Hinke de Rond, Lia Beckers, Lisa Eggink, Dirk van Tol, Sophie Reimerink, Johan Boer, Mardi van Beek, Josine Rots, Nynke van Binnendijk, Rob Buisman, Anne-Marie Vaccines (Basel) Article The generation of a specific long-term immune response to SARS-CoV-2 is considered important for protection against COVID-19 infection and disease. Memory B cells, responsible for the generation of antibody-producing plasmablasts upon a new antigen encounter, play an important role in this process. Therefore, the induction of memory B cell responses after primary and booster SARS-CoV-2 immunizations was investigated in the general population with an emphasis on older adults. Participants, 20–99 years of age, due to receive the mRNA-1273 or BNT162b2 SARS-CoV-2 vaccine were included in the current study. Specific memory B cells were determined by ex vivo ELISpot assays. In a subset of participants, antibody levels, avidity, and virus neutralization capacity were compared to memory B cell responses. Memory B cells specific for both Spike S1 and receptor-binding domain (RBD) were detected in the majority of participants following the primary immunization series. However, a proportion of predominantly older adults showed low frequencies of specific memory B cells. Booster vaccination resulted in a large increase in the frequencies of S1- and RBD-specific memory B cells also for those in which low memory B cell frequencies were detected after the primary series. These data show that booster immunization is important for the generation of a memory B cell response, as a subset of older adults shows a suboptimal response to the primary SARS-CoV-2 immunization series. It is anticipated that these memory B cells will play a significant role in the immune response following viral re-exposure. MDPI 2023-07-04 /pmc/articles/PMC10384172/ /pubmed/37515012 http://dx.doi.org/10.3390/vaccines11071196 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Verheul, Marije K.
Nijhof, Kim H.
de Zeeuw-Brouwer, Mary-lène
Duijm, Geraly
ten Hulscher, Hinke
de Rond, Lia
Beckers, Lisa
Eggink, Dirk
van Tol, Sophie
Reimerink, Johan
Boer, Mardi
van Beek, Josine
Rots, Nynke
van Binnendijk, Rob
Buisman, Anne-Marie
Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title_full Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title_fullStr Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title_full_unstemmed Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title_short Booster Immunization Improves Memory B Cell Responses in Older Adults Unresponsive to Primary SARS-CoV-2 Immunization
title_sort booster immunization improves memory b cell responses in older adults unresponsive to primary sars-cov-2 immunization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384172/
https://www.ncbi.nlm.nih.gov/pubmed/37515012
http://dx.doi.org/10.3390/vaccines11071196
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