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Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA

A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for...

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Autores principales: Ryabov, Eugene V., Nearman, Anthony J., Nessa, Ashrafun, Grubbs, Kyle, Sallmann, Benjamin, Fahey, Rachel, Wilson, Mikayla E., Rennich, Karen D., Steinhauer, Nathalie, Fauvel, Anne Marie, Chen, Yanping, Evans, Jay D., vanEngelsdorp, Dennis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384192/
https://www.ncbi.nlm.nih.gov/pubmed/37515283
http://dx.doi.org/10.3390/v15071597
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author Ryabov, Eugene V.
Nearman, Anthony J.
Nessa, Ashrafun
Grubbs, Kyle
Sallmann, Benjamin
Fahey, Rachel
Wilson, Mikayla E.
Rennich, Karen D.
Steinhauer, Nathalie
Fauvel, Anne Marie
Chen, Yanping
Evans, Jay D.
vanEngelsdorp, Dennis
author_facet Ryabov, Eugene V.
Nearman, Anthony J.
Nessa, Ashrafun
Grubbs, Kyle
Sallmann, Benjamin
Fahey, Rachel
Wilson, Mikayla E.
Rennich, Karen D.
Steinhauer, Nathalie
Fauvel, Anne Marie
Chen, Yanping
Evans, Jay D.
vanEngelsdorp, Dennis
author_sort Ryabov, Eugene V.
collection PubMed
description A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for a polyprotein with the protease, helicase, and RNA-dependent RNA polymerase domains, as well as a single jelly-roll structural protein domain, showing highest similarity with viruses in the family Solinviviridae. The injection of honey bee pupae with AmSV1 preparation showed an increase in virus titer and the accumulation of the negative-strand of AmSV1 RNA 3 days after injection, indicating the replication of AmSV1. In the infected worker bees, AmSV1 was present in heads, thoraxes, and abdomens, indicating that this virus causes systemic infection. An analysis of the geographic and historic distribution of AmSV1, using over 900 apiary samples collected across the United States, showed AmSV1 presence since at least 2010. In the year 2021, AmSV1 was detected in 10.45% of apiaries (95%CI: 8.41–12.79%), mostly sampled in June and July in Northwestern and Northeastern United States. The diagnostic methods and information on the AmSV1 distribution will be used to investigate the connection of AmSV1 to honey bee colony losses.
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spelling pubmed-103841922023-07-30 Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA Ryabov, Eugene V. Nearman, Anthony J. Nessa, Ashrafun Grubbs, Kyle Sallmann, Benjamin Fahey, Rachel Wilson, Mikayla E. Rennich, Karen D. Steinhauer, Nathalie Fauvel, Anne Marie Chen, Yanping Evans, Jay D. vanEngelsdorp, Dennis Viruses Article A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for a polyprotein with the protease, helicase, and RNA-dependent RNA polymerase domains, as well as a single jelly-roll structural protein domain, showing highest similarity with viruses in the family Solinviviridae. The injection of honey bee pupae with AmSV1 preparation showed an increase in virus titer and the accumulation of the negative-strand of AmSV1 RNA 3 days after injection, indicating the replication of AmSV1. In the infected worker bees, AmSV1 was present in heads, thoraxes, and abdomens, indicating that this virus causes systemic infection. An analysis of the geographic and historic distribution of AmSV1, using over 900 apiary samples collected across the United States, showed AmSV1 presence since at least 2010. In the year 2021, AmSV1 was detected in 10.45% of apiaries (95%CI: 8.41–12.79%), mostly sampled in June and July in Northwestern and Northeastern United States. The diagnostic methods and information on the AmSV1 distribution will be used to investigate the connection of AmSV1 to honey bee colony losses. MDPI 2023-07-21 /pmc/articles/PMC10384192/ /pubmed/37515283 http://dx.doi.org/10.3390/v15071597 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ryabov, Eugene V.
Nearman, Anthony J.
Nessa, Ashrafun
Grubbs, Kyle
Sallmann, Benjamin
Fahey, Rachel
Wilson, Mikayla E.
Rennich, Karen D.
Steinhauer, Nathalie
Fauvel, Anne Marie
Chen, Yanping
Evans, Jay D.
vanEngelsdorp, Dennis
Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title_full Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title_fullStr Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title_full_unstemmed Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title_short Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
title_sort apis mellifera solinvivirus-1, a novel honey bee virus that remained undetected for over a decade, is widespread in the usa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384192/
https://www.ncbi.nlm.nih.gov/pubmed/37515283
http://dx.doi.org/10.3390/v15071597
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