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Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA
A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384192/ https://www.ncbi.nlm.nih.gov/pubmed/37515283 http://dx.doi.org/10.3390/v15071597 |
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author | Ryabov, Eugene V. Nearman, Anthony J. Nessa, Ashrafun Grubbs, Kyle Sallmann, Benjamin Fahey, Rachel Wilson, Mikayla E. Rennich, Karen D. Steinhauer, Nathalie Fauvel, Anne Marie Chen, Yanping Evans, Jay D. vanEngelsdorp, Dennis |
author_facet | Ryabov, Eugene V. Nearman, Anthony J. Nessa, Ashrafun Grubbs, Kyle Sallmann, Benjamin Fahey, Rachel Wilson, Mikayla E. Rennich, Karen D. Steinhauer, Nathalie Fauvel, Anne Marie Chen, Yanping Evans, Jay D. vanEngelsdorp, Dennis |
author_sort | Ryabov, Eugene V. |
collection | PubMed |
description | A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for a polyprotein with the protease, helicase, and RNA-dependent RNA polymerase domains, as well as a single jelly-roll structural protein domain, showing highest similarity with viruses in the family Solinviviridae. The injection of honey bee pupae with AmSV1 preparation showed an increase in virus titer and the accumulation of the negative-strand of AmSV1 RNA 3 days after injection, indicating the replication of AmSV1. In the infected worker bees, AmSV1 was present in heads, thoraxes, and abdomens, indicating that this virus causes systemic infection. An analysis of the geographic and historic distribution of AmSV1, using over 900 apiary samples collected across the United States, showed AmSV1 presence since at least 2010. In the year 2021, AmSV1 was detected in 10.45% of apiaries (95%CI: 8.41–12.79%), mostly sampled in June and July in Northwestern and Northeastern United States. The diagnostic methods and information on the AmSV1 distribution will be used to investigate the connection of AmSV1 to honey bee colony losses. |
format | Online Article Text |
id | pubmed-10384192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103841922023-07-30 Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA Ryabov, Eugene V. Nearman, Anthony J. Nessa, Ashrafun Grubbs, Kyle Sallmann, Benjamin Fahey, Rachel Wilson, Mikayla E. Rennich, Karen D. Steinhauer, Nathalie Fauvel, Anne Marie Chen, Yanping Evans, Jay D. vanEngelsdorp, Dennis Viruses Article A metagenomic analysis of the virome of honey bees (Apis mellifera) from an apiary with high rates of unexplained colony losses identified a novel RNA virus. The virus, which was named Apis mellifera solinvivirus 1 (AmSV1), contains a 10.6 kb positive-strand genomic RNA with a single ORF coding for a polyprotein with the protease, helicase, and RNA-dependent RNA polymerase domains, as well as a single jelly-roll structural protein domain, showing highest similarity with viruses in the family Solinviviridae. The injection of honey bee pupae with AmSV1 preparation showed an increase in virus titer and the accumulation of the negative-strand of AmSV1 RNA 3 days after injection, indicating the replication of AmSV1. In the infected worker bees, AmSV1 was present in heads, thoraxes, and abdomens, indicating that this virus causes systemic infection. An analysis of the geographic and historic distribution of AmSV1, using over 900 apiary samples collected across the United States, showed AmSV1 presence since at least 2010. In the year 2021, AmSV1 was detected in 10.45% of apiaries (95%CI: 8.41–12.79%), mostly sampled in June and July in Northwestern and Northeastern United States. The diagnostic methods and information on the AmSV1 distribution will be used to investigate the connection of AmSV1 to honey bee colony losses. MDPI 2023-07-21 /pmc/articles/PMC10384192/ /pubmed/37515283 http://dx.doi.org/10.3390/v15071597 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ryabov, Eugene V. Nearman, Anthony J. Nessa, Ashrafun Grubbs, Kyle Sallmann, Benjamin Fahey, Rachel Wilson, Mikayla E. Rennich, Karen D. Steinhauer, Nathalie Fauvel, Anne Marie Chen, Yanping Evans, Jay D. vanEngelsdorp, Dennis Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title | Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title_full | Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title_fullStr | Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title_full_unstemmed | Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title_short | Apis mellifera Solinvivirus-1, a Novel Honey Bee Virus That Remained Undetected for over a Decade, Is Widespread in the USA |
title_sort | apis mellifera solinvivirus-1, a novel honey bee virus that remained undetected for over a decade, is widespread in the usa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384192/ https://www.ncbi.nlm.nih.gov/pubmed/37515283 http://dx.doi.org/10.3390/v15071597 |
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